Abstract
Effect of growing seedling, seeds and seedlings extracts from seven leguminous plants (Pisum sativum, Vigna radiata, Vigna mungo, Cajanus cajan, Lentil culinaris, Cicer arietinum and Trigonella foenum graecum) were screened for their ability to influence quorum sensing controlled pigment production in Chromobacterium violaceum indicator strains (CV12472 and CVO26). Germinating seedling and seedling extracts of only P. sativum (pea) showed inhibition of violacein production. Interestingly, the T. foenum graecum (fenugreek) seed extracts enhances the pigment production. Quorum sensing regulated swarming motility in Pseudomonas aerugionsa PAO1 was reduced by pea seedling extract while enhanced by the fenugreek seed extracts. These findings suggest that plant metabolites of some legumes interact actively with bacterial quorum sensing and could modulate its associated functions.
Keywords: Legume, Seedling and seed extracts, Quorum sensing modulation, Chromobacterium violaceum, Swarming motility
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References
- 1.Whiteland N.A., Barnard A.M., Slatrer H., Simpson N.J., Salmond G.P. Quorum sensing in Gram negative bacteria. FEMS (Fed Eur Microbiol Soc) Rev. 2001;25:365–404. doi: 10.1111/j.1574-6976.2001.tb00583.x. [DOI] [PubMed] [Google Scholar]
- 2.Fray R.G. Altering plant-microbe interaction through artificially manipulating bacterial quorum sensing. Ann Bot. 2002;89(3):245–253. doi: 10.1093/aob/mcf039. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Fuqua W.C., Winnans S.C. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J Bacteriol. 1994;176(10):2796–2806. doi: 10.1128/jb.176.10.2796-2806.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Pierson E.A., Wood D.W., Cannon J.A., Blachere F.M., Pierson L.S., IIIInterpopulation signaling via N-acyl-homoserine lactones among bacteria in the wheat rhizosphere Mol Plant Microbe Interact 1998111078–1084.10.1094/MPMI.1998.11.11.1078 [Google Scholar]
- 5.Teplitski M., Robinson J.B., Bauer W.D. Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. Mol Plant Microbe Interact. 2000;13(6):637–648. doi: 10.1094/MPMI.2000.13.6.637. [DOI] [PubMed] [Google Scholar]
- 6.Manefield M., Nys R., Kumar N., Read R., Givskov M., Steinberg P., Kjelleberg S. Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. Microbiology. 1999;145:283–291. doi: 10.1099/13500872-145-2-283. [DOI] [PubMed] [Google Scholar]
- 7.Vattem D.A., Mihali K.K., Crixell S.H., Mclean R.J. Dietary phytochemicals as quorum sensing inhibitors. Fitoterapia. 2007;78(4):302–310. doi: 10.1016/j.fitote.2007.03.009. [DOI] [PubMed] [Google Scholar]
- 8.Adonizio A.L., Downum K., Bennett B.C., Mathee K. Anti-quorum sensing activity of medicinal plants in Southern Florida. J Ethnopharmacol. 2006;105(3):427–435. doi: 10.1016/j.jep.2005.11.025. [DOI] [PubMed] [Google Scholar]
- 9.Steindler L., Venturi V. Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol Lett. 2007;266(1):1–9. doi: 10.1111/j.1574-6968.2006.00501.x. [DOI] [PubMed] [Google Scholar]
- 10.Shaw P.D., Ping G., Daly S., Cronan J.E., Rinehart K., Farrand S.K. Detecting and characterising acyl homoserine lactone signal molecules by thin layer chromatography. Proc Natl Acad Sci USA. 1997;94(12):6036–6041. doi: 10.1073/pnas.94.12.6036. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Ahmad I., Beg A.Z. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multidrug resistant human pathogens. J Ethnopharmacol. 2001;74:113–123. doi: 10.1016/s0378-8741(00)00335-4. [DOI] [PubMed] [Google Scholar]
- 12.Zhu H., Sun S.J. Inhibition of bacterial quorum sensing regulated behaviours bny Termella fuciformis extracts. Current Microbiology. 2008;57(5):418–422. doi: 10.1007/s00284-008-9215-8. [DOI] [PubMed] [Google Scholar]
- 13.Rasmussen T.B., Bjarnsholt T., Skindersoe M.E., Hentzer M., Kristoffersen P., Kote M., Nielson J., Eberl L., Givskov M. Screening for quorum sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bactetriol. 1995;187(5):1799–1814. doi: 10.1128/JB.187.5.1799-1814.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]