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. 2010 Mar 5;50(2):156–164. doi: 10.1007/s12088-010-0001-5

Biodegradation of Green HE4B: Co-substrate effect, biotransformation enzymes and metabolite toxicity analysis

S D Kalme 1,3, S U Jadhav 2, G K Parshetti 1, S P Govindwar 1,✉
PMCID: PMC3450328  PMID: 23100822

Abstract

A high exhaust reactive dye, Green HE4B (GHE4B) was 98% degraded in nutrient medium by Pseudomonas desmolyticum NCIM 2112 (pd2112) within 72 h at static condition. Decolorization time in synthetic 10 g/l molasses. Addition of 5 g/l peptone to NaCl medium had reduced decolorization time from 108 to 72 h. Beef extract do not contribute more to the inducing effect of peptone, however it is a good co-substrate in sucrose or urea containing NaCl medium. Intracellular lignin peroxidase (Lip), laccase and tyrosinase activities were induced by 150, 355 and 212%, respectively till maximum dye removal took place. Aminopyrine N-demethylase (AND) and dichlorophenol indophenol reductase (DCIP-reductase) activities in pd2112 were induced by 130 and 20%, respectively at 72 h of incubation during GHE4B decolorization. By high performance liquid chromatography (HPLC) analysis, 4-hydroxybenzene sulfonic acid and 4-amino, 6-hydroxynaphthalene 2-sulfonic acids were identified as metabolites formed during 24–72 h incubation. Fourier transform infrared spectroscopy (FTIR) analysis supports the formation of these aromatic amines. pd2112, aerobically degraded GHE4B metabolites (formed at static condition) showing stationary phase of 6 days. There was no germination inhibition of Sorghum bicolor and Triticum aestivum by GHE4B metabolites at 3,000 ppm concentration however untreated dye showed germination inhibition at the same concentration. GHE4B metabolites did not show any microbial toxicity at 10,000 ppm concentration.

Keywords: Biodegradation, Co-substrate, Green HE4B, Reactive dye, Toxicity

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References

  • 1.Pearce C.I., Lloyd J.R., Guthrie J.T. The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes Pigments. 2003;58:179–196. doi: 10.1016/S0143-7208(03)00064-0. [DOI] [Google Scholar]
  • 2.Grover I.S., Kaur A., Mahajan R.K. Mutagenicity of some dye effluents. Nat Aca Sci Lett India. 1996;19:149–158. [Google Scholar]
  • 3.Jadhav S.U., Kalme S.D., Govindwar S.P. Biodegradation of Methyl red by Galactomyces geotrichum MTCC 1360. Inter Biodete Biodeg. 2008;62:135–142. doi: 10.1016/j.ibiod.2007.12.010. [DOI] [Google Scholar]
  • 4.Moosvi S., Kcharia H., Madamwar D. Decolorization of textile dye Reactive Violet 5 by a newly isolated bacterial consortium RVM 11.1. W J Microiol Biotechnol. 2005;21:667–672. doi: 10.1007/s11274-004-3612-3. [DOI] [Google Scholar]
  • 5.Supaka N., Juntongjin K., Damronglerd S., Delia M., Strehaiano P. Microbial decolorization of reactive azo dyes in a sequential anaerobic-aerobic system. Chem Eng J. 2004;99:169–176. doi: 10.1016/j.cej.2003.09.010. [DOI] [Google Scholar]
  • 6.Kalme S.D., Parshetti G.K., Jadhav S.U., Govindwar S.P. Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Biores Technol. 2007;98:1405–1410. doi: 10.1016/j.biortech.2006.05.023. [DOI] [PubMed] [Google Scholar]
  • 7.Standard Methods for Examination of Water and Wastewater. 19th edition. Washington, DC, USA: APHA, AWWA and WPCF; 1995. [Google Scholar]
  • 8.Saratale G.D., Kalme S.D., Govindwar S.P. Decolorization of textile dyes by Aspergillus ochraceus. Ind J Biotechnol. 2006;5:407–410. [Google Scholar]
  • 9.Kalme S., Ghodake G., Govindwar S. Red HE7B degradation using desulfonation by Pseudomonas desmolyticum NCIM 2112. Int Biodet Biodeg. 2007;60:327–333. doi: 10.1016/j.ibiod.2007.05.006. [DOI] [Google Scholar]
  • 10.Kalme S. Role of microbes in biotransformation of chemicals. Kolhapur, India: Shivaji University; 2006. p. 47. [Google Scholar]
  • 11.Hatvani N., Mecs I. Production of laccase and manganese peroxidase by Lentinus edodes on malt containing by product of the brewing process. Process Biochem. 2001;37:491–496. doi: 10.1016/S0032-9592(01)00236-9. [DOI] [Google Scholar]
  • 12.Zhang X., Flurkey W. Phenoloxidases in Portabella mushrooms. J Food Sci. 1997;62:97–100. doi: 10.1111/j.1365-2621.1997.tb04376.x. [DOI] [Google Scholar]
  • 13.Kalme S.D., Parshetti G.K., Gomare S.S., Govindwar S.P. Diesel and Kerosene degradation by diesel and kerosene degradation by Pseudomonas desmolyticum NCIM 2112 and Nocardia hydrocarbonoxydans NCIM 2386. Curr Microbiol. 2008;56:581–586. doi: 10.1007/s00284-008-9128-6. [DOI] [PubMed] [Google Scholar]
  • 14.Parshetti G., Kalme S., Saratale G., Govindwar S. Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chim Slov. 2006;53:492–498. [Google Scholar]
  • 15.Nicholas D.J.D., Fisher D.J. Nitrogen fixation in extracts of Azotobacter vinelandii. Nature. 1960;186:735–736. doi: 10.1038/186735a0. [DOI] [PubMed] [Google Scholar]
  • 16.Dean S.M., Jin Y., Cha D.K., Wilson S.V., Radosevich M. Phenanthrene degradation in soils co-inoculated with phenanthrene-degrading and biosurfactant-producing bacteria. J Environ Qual. 2001;30:1126–1133. doi: 10.2134/jeq2001.3041126x. [DOI] [PubMed] [Google Scholar]
  • 17.Jadhav S.U., Jadhav U.U., Dawkar W., Govindwar S.P. Biodegradation of Disperse Dye Brown 3REL by 14. microbial consortium of Galactomyces geotrichum MTCC 1360 and Bacillus sp. VUS. Biotechnol Biopro Engg. 2008;13:232–239. doi: 10.1007/s12257-007-0204-8. [DOI] [Google Scholar]
  • 18.Lee T.H., Aoki H., Sugano Y., Shoda M. Effect of molasses on the production and activity of dye-decolorizing peroxidase from Geotrichum candidum Dec l. J Biosci Bioeng. 2000;89:545–549. doi: 10.1016/S1389-1723(00)80054-7. [DOI] [PubMed] [Google Scholar]
  • 19.Dong X., Zhou J., Liu Y. Peptone-induced biodecolorization of Reactive Brilliant Blue (KN-R) by Rhodocyclus gelatinosus XL-1. Process Biochem. 2003;39:89–94. doi: 10.1016/S0032-9592(02)00319-9. [DOI] [Google Scholar]
  • 20.Kalyani D.C., Patil P.S., Jadhav J.P., Govindwar S.P. Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Biores Technol. 2008;99:4635–4641. doi: 10.1016/j.biortech.2007.06.058. [DOI] [PubMed] [Google Scholar]
  • 21.Nachiyar C.V., Rajkumar G.S. Degradation of a tannery and textile dye, Navitan Fast Blue S5R by Pseudomonas aeruginosa. W J Microbiol Biotechnol. 2003;19:609–614. doi: 10.1023/A:1025159617260. [DOI] [Google Scholar]
  • 22.Yuri L., Park C., Lee B., Han E.-J., Kim T.-H., Lee J., Kim S. Effect of nutrients on the production of extracellular enzymes for decolorization of Reactive Blue 19 and Reactive Black 5. J Microbiol Biotechnol. 2006;16:226–231. [Google Scholar]
  • 23.McMahon A.M., Doyle E.M., Brooks S., O’Connor K.E. Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6. Enz Microb Technol. 2007;40:1435–1441. doi: 10.1016/j.enzmictec.2006.10.020. [DOI] [Google Scholar]
  • 24.Kandelbauer A., Erlacher A., Cavaco-Paulo A., Guebitz G. Laccase-catalyzed decolorization of the synthetic azo dye Diamond Black PV 200 and of some structurally related derivatives. Biocat Biotrans. 2004;22:331–339. doi: 10.1080/10242420400024573. [DOI] [Google Scholar]
  • 25.Maier J., Kandelbauer A., Erlacher A., Cavaco-Paulo A., Gübitz G. A new alkali-thermostable azoreductase from Bacillus sp. strain SF. Appl Env Microbiol. 2004;70:837–844. doi: 10.1128/AEM.70.2.837-844.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Kneifel H., Elmendorff K., Hegewald E., Soeder C.J. Biotransformation of 1-naphthalenesulfonic acid by the green alga Scenedesmus obliquus. Arch Microbiol. 1997;167:32–37. doi: 10.1007/s002030050413. [DOI] [PubMed] [Google Scholar]
  • 27.Ekiei P., Leupold G., Parlar H. Degradability of selected azo dye metabolite in activated sludge systems. Chemosphere. 2001;44:721–728. doi: 10.1016/S0045-6535(00)00345-3. [DOI] [PubMed] [Google Scholar]

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