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. 1984 Apr;81(8):2406–2410. doi: 10.1073/pnas.81.8.2406

Chemically defined media for cultivation of Naegleria gruberi

Chandler Fulton 1,*, Cecelia Webster 1, Jennifer S Wu 1
PMCID: PMC345069  PMID: 16593456

Abstract

Cultivation of amebae of the axenic strain of Naegleria gruberi, NEG-M, was achieved in media consisting entirely of chemically defined components. A complete medium that contains 31 components allows growth with yields up to 5 × 106 amebae per ml. A minimal medium gives lower yields but defines 22 components that are essential for continuous cultivation: 11 amino acids, 6 vitamins, hematin, guanosine, D-glucose, Mg2+, and inorganic phosphate. These media allow precise studies of the metabolism and differentiation of this unusual eukaryote.

Keywords: amebae, minimal medium, nutritional requirements

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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