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. 1984 Apr;81(8):2452–2455. doi: 10.1073/pnas.81.8.2452

Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis.

M R Wabl, P D Burrows
PMCID: PMC345079  PMID: 6326129

Abstract

The major intron between the J and C gene segments of the immunoglobulin heavy (H) chain locus contains an enhancer-like sequence, and it has been proposed that this enhancer is necessary to achieve high levels of H chain expression. We have isolated a subclone of the lymphoid pre-B cell line 18-81 that lacks this enhancer but nevertheless produces mu chain at the level characteristic of pre-B cells. Another subclone with a larger deletion does not produce mu chain, but upon fusion with a myeloma that does not produce any immunoglobulin chain, mu chain is expressed by the homolog from the pre-B subclone. The hybridoma lacks the proposed enhancer element in cis; nevertheless it produces as much mu chain as other plasma cell hybridomas. Therefore, this enhancer element is not obligatory for a high level of H chain production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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