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. 1984 Apr;81(8):2466–2469. doi: 10.1073/pnas.81.8.2466

Role of cell surface immunoglobulin in B-lymphocyte activation.

N J LoCascio, G Haughton, L W Arnold, R B Corley
PMCID: PMC345082  PMID: 6232610

Abstract

The role of cell surface immunoglobulin in helper T-cell-dependent B-cell activation was analyzed using a B-cell lymphoma, CH12, with known antigen specificity and activation properties similar to those of a resting B cell. Two sources of helper T cells were used, both selected such that they interact with H-2-encoded determinants on CH12 in the absence of the specific B-cell antigen, sheep erythrocytes. By this dissociation of the specificity of the T cells from that of the B cells, the requirement for antigen in the induction of CH12 to antibody secretion could be studied. The results show that both helper T-cell-B-cell interactions and surface immunoglobulin-antigen binding are involved in inducing B-cell differentiation, thus establishing a signalling function for the antigen receptor on B lymphocytes. Our data also show that the requirement for surface immunoglobulin-ligand interactions in B-cell activation can, under certain conditions, be circumvented, notably when high (nonphysiologic) multiplicities of T-cell help are used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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