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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Apr;81(8):2494–2498. doi: 10.1073/pnas.81.8.2494

Ly-1 B cells: functionally distinct lymphocytes that secrete IgM autoantibodies.

K Hayakawa, R R Hardy, M Honda, L A Herzenberg, A D Steinberg, L A Herzenberg
PMCID: PMC345088  PMID: 6609363

Abstract

Studies presented here introduce another perspective on the mechanisms responsible for IgM autoantibody production. A unique subpopulation of B lymphocytes (Ly-1 B) that concomitantly expresses IgM, IgD, Ia, and Ly-1 membrane glycoproteins is present at higher frequencies in NZB and NZB-related mice. The Ly-1 B subpopulation in these autoimmune animals is responsible for the "spontaneous" IgM secretion demonstrated with cultured NZB spleen cells and contains the cells that secrete typical NZB IgM autoantibodies to single-stranded DNA and to thymocytes. In addition, the Ly-1 B population in normal mouse strains (and in NZB) contains virtually all of the spleen cells that secrete IgM autoantibodies reactive with bromelain-treated mouse erythrocytes. Since a different B-cell subpopulation (IgM+, IgD-, Ly-1) secretes most of the IgM antibodies produced in responses to exogenous antigens, we conclude that Ly-1 B cells constitute a functionally distinct B-cell population important in certain kinds of autoimmunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cunningham A. J. Large numbers of cells in normal mice produce antibody components of isologous erythrocytes. Nature. 1974 Dec 20;252(5485):749–751. doi: 10.1038/252749a0. [DOI] [PubMed] [Google Scholar]
  2. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  3. DeHeer D. H., Linder E. J., Edgington T. S. Delineation of spontaneous erythrocyte autoantibody responses of NZB and other strains of mice. J Immunol. 1978 Mar;120(3):825–830. [PubMed] [Google Scholar]
  4. Dresser D. W. Most IgM-producing cells in the mouse secrete auto-antibodies (rheumatoid factor). Nature. 1978 Aug 3;274(5670):480–483. doi: 10.1038/274480a0. [DOI] [PubMed] [Google Scholar]
  5. Hammarström L., Smith E., Primi D., Möller G. Induction of autoantibodies to red blood cells by polyclonal B-cell activators. Nature. 1976 Sep 2;263(5572):60–61. doi: 10.1038/263060a0. [DOI] [PubMed] [Google Scholar]
  6. Hang L., Slack J. H., Amundson C., Izui S., Theofilopoulos A. N., Dixon F. J. Induction of murine autoimmune disease by chronic polyclonal B cell activation. J Exp Med. 1983 Mar 1;157(3):874–883. doi: 10.1084/jem.157.3.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hardy R. R., Hayakawa K., Haaijman J., Herzenberg L. A. B-cell subpopulations identified by two-colour fluorescence analysis. Nature. 1982 Jun 17;297(5867):589–591. doi: 10.1038/297589a0. [DOI] [PubMed] [Google Scholar]
  8. Hayakawa K., Hardy R. R., Parks D. R., Herzenberg L. A. The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med. 1983 Jan 1;157(1):202–218. doi: 10.1084/jem.157.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Izui S., McConahey P. J., Dixon F. J. Increased spontaneous polyclonal activation of B lymphocytes in mice with spontaneous autoimmune disease. J Immunol. 1978 Dec;121(6):2213–2219. [PubMed] [Google Scholar]
  10. Jacobs D. M., Morrison D. C. Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS). J Immunol. 1975 Jan;114(1 Pt 2):360–364. [PubMed] [Google Scholar]
  11. Manohar V., Brown E., Leiserson W. M., Chused T. M. Expression of Lyt-1 by a subset of B lymphocytes. J Immunol. 1982 Aug;129(2):532–538. [PubMed] [Google Scholar]
  12. Pages J., Bussard A. E. Precommitment of normal mouse peritoneal cells by erythrocyte antigens in relation to auto-antibody production. Nature. 1975 Sep 25;257(5524):316–317. doi: 10.1038/257316a0. [DOI] [PubMed] [Google Scholar]
  13. Shirai T., Mellors R. C. Natural thymocytotoxic autoantibody and reactive antigen in New Zealand black and other mice. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1412–1415. doi: 10.1073/pnas.68.7.1412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steele E. J., Cunningham A. J. High proportion of Ig-producing cells making autoantibody in normal mice. Nature. 1978 Aug 3;274(5670):483–484. doi: 10.1038/274483a0. [DOI] [PubMed] [Google Scholar]
  15. Steinberg E. B., Santoro T. J., Chused T. M., Smathers P. A., Steinberg A. D. Studies of congenic MRL-Ipr/Ipr.xid mice. J Immunol. 1983 Dec;131(6):2789–2795. [PubMed] [Google Scholar]

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