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. 1984 May;81(9):2757–2761. doi: 10.1073/pnas.81.9.2757

Mechanism of the concerted action of recA protein and helix-destabilizing proteins in homologous recombination.

K Muniyappa, S L Shaner, S S Tsang, C M Radding
PMCID: PMC345149  PMID: 6326142

Abstract

Secondary structure in single-stranded DNA impedes the presynaptic association of recA protein and consequently blocks the formation of joint molecules as evidenced by effects of temperature, nucleotide sequence, and ionic conditions. Escherichia coli single-strand-binding protein eliminates sequence-specific "cold spots" by removing folds even from sites of strong secondary structure. Thus, destabilization of secondary structure in single-stranded DNA is critical for the action of recA protein, whereas specific interactions directly between helix-destabilizing proteins and recA protein are unimportant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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