Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 May;81(9):2934–2937. doi: 10.1073/pnas.81.9.2934

Evidence for parallel actions of a molluscan neuropeptide and serotonin in mediating arousal in Aplysia.

P E Lloyd, I Kupfermann, K R Weiss
PMCID: PMC345188  PMID: 6326155

Abstract

The neuropeptide designated SCPB (small cardioactive peptide B), the sequence of which has recently been determined, was found in the accessory radula closer muscle, a muscle involved in biting movements. The ganglia and nerves that innervate the accessory radula closer muscle also contain SCPB. At nanomolar concentrations, it enhances the contractions of the muscle. The effect of SCPB on the muscle resembles the effect of an identified serotonergic neuron that previously was shown to mediate behavioral effects that reflect a food arousal state in Aplysia. Like serotonin, SCPB enhances contractions by a postsynaptic action, which appears to involve an increase in cAMP levels in the muscle. Our findings suggest that parallel peptidergic and serotonergic pathways may mediate similar aspects of arousal in Aplysia.

Full text

PDF
2934

Images in this article

2935Image
on p.2935

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams M. E., O'Shea M. Peptide cotransmitter at a neuromuscular junction. Science. 1983 Jul 15;221(4607):286–289. doi: 10.1126/science.6134339. [DOI] [PubMed] [Google Scholar]
  2. Chan-Palay V., Engel A. G., Wu J. Y., Palay S. L. Coexistence in human and primate neuromuscular junctions of enzymes synthesizing acetylcholine, catecholamine, taurine, and gamma-aminobutyric acid. Proc Natl Acad Sci U S A. 1982 Nov;79(22):7027–7030. doi: 10.1073/pnas.79.22.7027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen J. L., Weiss K. R., Kupfermann I. Motor control of buccal muscles in Aplysia. J Neurophysiol. 1978 Jan;41(1):157–180. doi: 10.1152/jn.1978.41.1.157. [DOI] [PubMed] [Google Scholar]
  4. Goldstein R., Kistler H. B., Jr, Steinbusch H. W., Schwartz J. H. Distribution of serotonin-immunoreactivity in juvenile Aplysia. Neuroscience. 1984 Feb;11(2):535–547. doi: 10.1016/0306-4522(84)90043-5. [DOI] [PubMed] [Google Scholar]
  5. Greenberg M. J., Painter S. D., Doble K. E., Nagle G. T., Price D. A., Lehman H. K. The molluscan neurosecretory peptide FMRFamide: comparative pharmacology and relationship to the enkephalins. Fed Proc. 1983 Jan;42(1):82–86. [PubMed] [Google Scholar]
  6. Hökfelt T., Johansson O., Ljungdahl A., Lundberg J. M., Schultzberg M. Peptidergic neurones. Nature. 1980 Apr 10;284(5756):515–521. doi: 10.1038/284515a0. [DOI] [PubMed] [Google Scholar]
  7. Kravitz E. A., Glusman S., Harris-Warrick R. M., Livingstone M. S., Schwarz T., Goy M. F. Amines and a peptide as neurohormones in lobsters: actions on neuromuscular preparations and preliminary behavioural studies. J Exp Biol. 1980 Dec;89:159–175. doi: 10.1242/jeb.89.1.159. [DOI] [PubMed] [Google Scholar]
  8. Kupfermann I. Dissociation of the appetitive and consummatory phases of feeding behavior in Aplysia: a lesion study. Behav Biol. 1974 Jan;10(1):89–97. doi: 10.1016/s0091-6773(74)91694-0. [DOI] [PubMed] [Google Scholar]
  9. Lloyd P. E. Cardioactive neuropeptides in gastropods. Fed Proc. 1982 Nov;41(13):2948–2952. [PubMed] [Google Scholar]
  10. Magistretti P. J., Morrison J. H., Shoemaker W. J., Sapin V., Bloom F. E. Vasoactive intestinal polypeptide induces glycogenolysis in mouse cortical slices: a possible regulatory mechanism for the local control of energy metabolism. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6535–6539. doi: 10.1073/pnas.78.10.6535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morris H. R., Panico M., Karplus A., Lloyd P. E., Riniker B. Elucidation by FAB-MS of the structure of a new cardioactive peptide from Aplysia. Nature. 1982 Dec 16;300(5893):643–645. doi: 10.1038/300643a0. [DOI] [PubMed] [Google Scholar]
  12. O'Shea M., Bishop C. A. Neuropeptide proctolin associated with an identified skeletal motoneuron. J Neurosci. 1982 Sep;2(9):1242–1251. doi: 10.1523/JNEUROSCI.02-09-01242.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Osborne N. N., Cuello A. C., Dockray G. J. Substance P and cholecystokinin-like peptides in Helix neurons and cholecystokinin and serotonin in a giant neuron. Science. 1982 Apr 23;216(4544):409–411. doi: 10.1126/science.6176023. [DOI] [PubMed] [Google Scholar]
  14. Sjöstrand N. O., Swedin G. Potentiation by smooth muscle stimulants of the hypogastric nerve--vas deferens preparation from normal and castrated guinea-pigs. Acta Physiol Scand. 1968 Nov;74(3):472–479. doi: 10.1111/j.1748-1716.1968.tb04255.x. [DOI] [PubMed] [Google Scholar]
  15. Weiss K. R., Cohen J. L., Kupfermann I. Modulatory control of buccal musculature by a serotonergic neuron (metacerebral cell) in Aplysia. J Neurophysiol. 1978 Jan;41(1):181–203. doi: 10.1152/jn.1978.41.1.181. [DOI] [PubMed] [Google Scholar]
  16. Weiss K. R., Kupfermann I. Homology of the giant serotonergic neurons (metacerebral cells) in Aplysia and pulmonate molluscs. Brain Res. 1976 Nov 19;117(1):33–49. doi: 10.1016/0006-8993(76)90554-0. [DOI] [PubMed] [Google Scholar]
  17. Weiss K. R., Mandelbaum D. E., Schonberg M., Kupfermann I. Modulation of buccal muscle contractility by serotonergic metacerebral cells in Aplysia: evidence for a role of cyclic adenosine monophosphate. J Neurophysiol. 1979 May;42(3):791–803. doi: 10.1152/jn.1979.42.3.791. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES