Abstract
Periodic patterns are observed when supported lipid monolayers doped with low concentrations of fluorescent lipid probes are observed with epi-fluorescence microscopy. Monolayers of dipalmitoyl phosphatidylcholine were examined on air-water interfaces and also on alkylated glass coverslips. The patterns are formed by periodic arrays of solid-phase lipid domains in equilibrium with fluid-phase lipid under specified conditions of temperature and two-dimensional lipid pressure. Electrostatic forces may stabilize the periodic ordering of the solid domains.
Keywords: two-dimensional systems, lateral diffusion, fluorescence microscopy, membranes
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- Balakrishnan K., Hsu F. J., Cooper A. D., McConnell H. M. Lipid hapten containing membrane targets can trigger specific immunoglobulin E-dependent degranulation of rat basophil leukemia cells. J Biol Chem. 1982 Jun 10;257(11):6427–6433. [PubMed] [Google Scholar]
- Cartwright G. S., Smith L. M., Heinzelmann E. W., Ruebush M. J., Parce J. W., McConnell H. M. H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1506–1510. doi: 10.1073/pnas.79.5.1506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grant C. W., Wu S. H., McConnell H. M. Lateral phase separations in binary lipid mixtures: correlation between spin label and freeze-fracture electron microscopic studies. Biochim Biophys Acta. 1974 Sep 6;363(2):151–158. doi: 10.1016/0005-2736(74)90055-8. [DOI] [PubMed] [Google Scholar]
- Griffin R. G. Solid state nuclear magnetic resonance of lipid bilayers. Methods Enzymol. 1981;72:108–174. doi: 10.1016/s0076-6879(81)72010-x. [DOI] [PubMed] [Google Scholar]
- Hafeman D. G., Lewis J. T., McConnell H. M. Triggering of the macrophage and neutrophil respiratory burst by antibody bound to a spin-label phospholipid hapten in model lipid bilayer membranes. Biochemistry. 1980 Nov 11;19(23):5387–5394. doi: 10.1021/bi00564a037. [DOI] [PubMed] [Google Scholar]
- Hafeman D. G., Smith L. M., Fearon D. T., McConnell H. M. Lipid monolayer-coated solid surfaces do not perturb the lateral motion and distribution of C3b receptors on neutrophils. J Cell Biol. 1982 Jul;94(1):224–227. doi: 10.1083/jcb.94.1.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hafeman D. G., von Tscharner V., McConnell H. M. Specific antibody-dependent interactions between macrophages and lipid haptens in planar lipid monolayers. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4552–4556. doi: 10.1073/pnas.78.7.4552. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakanishi M., Brian A. A., McConnell H. M. Binding of cytotoxic T-lymphocytes to supported lipid monolayers containing trypsinized H-2Kk. Mol Immunol. 1983 Nov;20(11):1227–1231. doi: 10.1016/0161-5890(83)90147-5. [DOI] [PubMed] [Google Scholar]
- Peters R., Beck K. Translational diffusion in phospholipid monolayers measured by fluorescence microphotolysis. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7183–7187. doi: 10.1073/pnas.80.23.7183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seul M., Eisenberger P., McConnell H. M. X-ray diffraction by phospholipid monolayers on single-crystal silicon substrates. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5795–5797. doi: 10.1073/pnas.80.18.5795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tardieu A., Luzzati V., Reman F. C. Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol. 1973 Apr 25;75(4):711–733. doi: 10.1016/0022-2836(73)90303-3. [DOI] [PubMed] [Google Scholar]
- Weis R. M., Balakrishnan K., Smith B. A., McConnell H. M. Stimulation of fluorescence in a small contact region between rat basophil leukemia cells and planar lipid membrane targets by coherent evanescent radiation. J Biol Chem. 1982 Jun 10;257(11):6440–6445. [PubMed] [Google Scholar]
- von Tscharner V., McConnell H. M. An alternative view of phospholipid phase behavior at the air-water interface. Microscope and film balance studies. Biophys J. 1981 Nov;36(2):409–419. doi: 10.1016/S0006-3495(81)84740-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Tscharner V., McConnell H. M. Physical properties of lipid monolayers on alkylated planar glass surfaces. Biophys J. 1981 Nov;36(2):421–427. doi: 10.1016/S0006-3495(81)84741-8. [DOI] [PMC free article] [PubMed] [Google Scholar]