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. 1984 Jun;81(12):3773–3777. doi: 10.1073/pnas.81.12.3773

Mutants suppressing in trans chorion gene amplification in Drosophila.

W Orr, K Komitopoulou, F C Kafatos
PMCID: PMC345302  PMID: 6427774

Abstract

Two recessive female-sterile mutants, K451 and K1214 , disrupt chorion formation by causing underproduction of all major chorion proteins. We present evidence that this effect is due to underaccumulation of the chorion mRNAs and that, in turn, this is caused by a substantial reduction in the level of chorion gene amplification. The mutants are X-linked but located at two sites far from the chorion gene cluster at 7F1 -2; their effect is even more pronounced on the third chromosome chorion gene cluster, and thus the wild type gene must act in trans. The time course of amplification in mutant and wild-type follicles is documented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beadle G. W., Ephrussi B. The Differentiation of Eye Pigments in Drosophila as Studied by Transplantation. Genetics. 1936 May;21(3):225–247. doi: 10.1093/genetics/21.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gans M., Audit C., Masson M. Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster. Genetics. 1975 Dec;81(4):683–704. doi: 10.1093/genetics/81.4.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Griffin-Shea R., Thireos G., Kafatos F. C. Organization of a cluster of four chorion genes in Drosophila and its relationship to developmental expression and amplification. Dev Biol. 1982 Jun;91(2):325–336. doi: 10.1016/0012-1606(82)90039-2. [DOI] [PubMed] [Google Scholar]
  5. Griffin-Shea R., Thireos G., Kafatos F. C., Petri W. H., Villa-Komaroff L. Chorion cDNA clones of D. melanogaster and their use in studies of sequence homology and chromosomal location of chorion genes. Cell. 1980 Apr;19(4):915–922. doi: 10.1016/0092-8674(80)90083-5. [DOI] [PubMed] [Google Scholar]
  6. Komitopoulou K., Gans M., Margaritis L. H., Kafatos F. C., Masson M. Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants. Genetics. 1983 Dec;105(4):897–920. doi: 10.1093/genetics/105.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Margaritis L. H., Kafatos F. C., Petri W. H. The eggshell of Drosophila melanogaster. I. Fine structure of the layers and regions of the wild-type eggshell. J Cell Sci. 1980 Jun;43:1–35. doi: 10.1242/jcs.43.1.1. [DOI] [PubMed] [Google Scholar]
  8. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Spradling A. C., Digan M. E., Mahowald A. P., Scott M., Craig E. A. Two clusters of genes for major chorion proteins of Drosophila melanogaster. Cell. 1980 Apr;19(4):905–914. doi: 10.1016/0092-8674(80)90082-3. [DOI] [PubMed] [Google Scholar]
  12. Spradling A. C., Mahowald A. P. A chromosome inversion alters the pattern of specific DNA replication in Drosophila follicle cells. Cell. 1981 Nov;27(1 Pt 2):203–209. doi: 10.1016/0092-8674(81)90374-3. [DOI] [PubMed] [Google Scholar]
  13. Spradling A. C., Mahowald A. P. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096–1100. doi: 10.1073/pnas.77.2.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spradling A. C., Mahowald A. P. Identification and genetic localization of mRNAs from ovarian follicle cells of Drosophila melanogaster. Cell. 1979 Mar;16(3):589–598. doi: 10.1016/0092-8674(79)90032-1. [DOI] [PubMed] [Google Scholar]
  15. Spradling A. C. The organization and amplification of two chromosomal domains containing Drosophila chorion genes. Cell. 1981 Nov;27(1 Pt 2):193–201. doi: 10.1016/0092-8674(81)90373-1. [DOI] [PubMed] [Google Scholar]
  16. Spradling A. C., Waring G. L., Mahowald A. P. Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene. Cell. 1979 Mar;16(3):609–616. doi: 10.1016/0092-8674(79)90034-5. [DOI] [PubMed] [Google Scholar]
  17. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waring G. L., Mahowald A. P. Identification and time of synthesis of chorion proteins in Drosophila melanogaster. Cell. 1979 Mar;16(3):599–607. doi: 10.1016/0092-8674(79)90033-3. [DOI] [PubMed] [Google Scholar]
  19. Wieslander L. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979 Oct 1;98(2):305–309. doi: 10.1016/0003-2697(79)90145-3. [DOI] [PubMed] [Google Scholar]

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