Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jun;81(12):3806–3810. doi: 10.1073/pnas.81.12.3806

A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.

J Yates, N Warren, D Reisman, B Sugden
PMCID: PMC345309  PMID: 6328526

Abstract

The Epstein-Barr viral (EBV) genome of approximately equal to 170 kilobase pairs (kbp) is maintained as a plasmid in human B lymphoblasts transformed by the virus. We have identified a cis-acting element within 1.8 kbp of the viral genome that allows recombinant plasmids carrying it to be selected at high frequency and maintained as plasmids in cells latently infected by EBV. This functional element(s) requires a segment of DNA at least 800 bp and at most 1800 bp long, which contains a family of 30-bp tandem repeats at one end. Since this region confers efficient stable replication only to plasmids transfected into cells containing EBV genomes, its function probably requires trans-acting products encoded elsewhere in the viral genome.

Full text

PDF
3806

Images in this article

3808Image
on p.3808
3809Image
on p.3809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Todaro G. J. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science. 1968 Nov 29;162(3857):1024–1026. doi: 10.1126/science.162.3857.1024. [DOI] [PubMed] [Google Scholar]
  2. Arrand J. R., Rymo L., Walsh J. E., Björck E., Lindahl T., Griffin B. E. Molecular cloning of the complete Epstein-Barr virus genome as a set of overlapping restriction endonuclease fragments. Nucleic Acids Res. 1981 Jul 10;9(13):2999–3014. doi: 10.1093/nar/9.13.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bingham A. H., Atkinson T., Sciaky D., Roberts R. J. A specific endonuclease from Bacillus caldolyticus. Nucleic Acids Res. 1978 Oct;5(10):3457–3467. doi: 10.1093/nar/5.10.3457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bozzoni I., Baldari C. T., Amaldi F., Buongiorno-Nardelli M. Replication of ribosomal DNA in Xenopus laevis. Eur J Biochem. 1981 Sep 1;118(3):585–590. doi: 10.1111/j.1432-1033.1981.tb05559.x. [DOI] [PubMed] [Google Scholar]
  5. Brent R., Ptashne M. The lexA gene product represses its own promoter. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932–1936. doi: 10.1073/pnas.77.4.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buell G. N., Reisman D., Kintner C., Crouse G., Sugden B. Cloning overlapping DNA fragments from the B95-8 strain of Epstein-Barr virus reveals a site of homology to the internal repetition. J Virol. 1981 Dec;40(3):977–982. doi: 10.1128/jvi.40.3.977-982.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Capecchi M. R. High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell. 1980 Nov;22(2 Pt 2):479–488. doi: 10.1016/0092-8674(80)90358-x. [DOI] [PubMed] [Google Scholar]
  8. Chu G., Sharp P. A. SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen. Gene. 1981 Mar;13(2):197–202. doi: 10.1016/0378-1119(81)90008-1. [DOI] [PubMed] [Google Scholar]
  9. Colbère-Garapin F., Horodniceanu F., Kourilsky P., Garapin A. C. A new dominant hybrid selective marker for higher eukaryotic cells. J Mol Biol. 1981 Jul 25;150(1):1–14. doi: 10.1016/0022-2836(81)90321-1. [DOI] [PubMed] [Google Scholar]
  10. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  11. Frost E., Williams J. Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology. 1978 Nov;91(1):39–50. doi: 10.1016/0042-6822(78)90353-7. [DOI] [PubMed] [Google Scholar]
  12. Glaser R., Nonoyama M. Epstein-Barr virus: detection of genome in somatic cell hybrids of Burkitt lymphoblastoid cells. Science. 1973 Feb 2;179(4072):492–493. doi: 10.1126/science.179.4072.492. [DOI] [PubMed] [Google Scholar]
  13. Glaser R., Nonoyama M. Host cell regulation of induction of Epstein-Barr virus. J Virol. 1974 Jul;14(1):174–176. doi: 10.1128/jvi.14.1.174-176.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Glaser R., Rapp F. Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells. J Virol. 1972 Aug;10(2):288–296. doi: 10.1128/jvi.10.2.288-296.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  16. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  17. Hattman S., Brooks J. E., Masurekar M. Sequence specificity of the P1 modification methylase (M.Eco P1) and the DNA methylase (M.Eco dam) controlled by the Escherichia coli dam gene. J Mol Biol. 1978 Dec 15;126(3):367–380. doi: 10.1016/0022-2836(78)90046-3. [DOI] [PubMed] [Google Scholar]
  18. Hay R. T., DePamphilis M. L. Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region. Cell. 1982 Apr;28(4):767–779. doi: 10.1016/0092-8674(82)90056-3. [DOI] [PubMed] [Google Scholar]
  19. Heintz N. H., Hamlin J. L. An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4083–4087. doi: 10.1073/pnas.79.13.4083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Henry B. E., 2nd, Raab-Traub N. J., Pagano J. S. Detection of autonomous replicating sequences (ars) in the genome of Epstein-Barr virus. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1096–1100. doi: 10.1073/pnas.80.4.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  22. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  23. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  24. Kaschka-Dierich C., Adams A., Lindahl T., Bornkamm G. W., Bjursell G., Klein G., Giovanella B. C., Singh S. Intracellular forms of Epstein-Barr virus DNA in human tumour cells in vivo. Nature. 1976 Mar 25;260(5549):302–306. doi: 10.1038/260302a0. [DOI] [PubMed] [Google Scholar]
  25. Kintner C., Sugden B. Conservation and progressive methylation of Epstein-Barr viral DNA sequences in transformed cells. J Virol. 1981 Apr;38(1):305–316. doi: 10.1128/jvi.38.1.305-316.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lindahl T., Adams A., Bjursell G., Bornkamm G. W., Kaschka-Dierich C., Jehn U. Covalently closed circular duplex DNA of Epstein-Barr virus in a human lymphoid cell line. J Mol Biol. 1976 Apr 15;102(3):511–530. doi: 10.1016/0022-2836(76)90331-4. [DOI] [PubMed] [Google Scholar]
  27. Luciw P. A., Bishop J. M., Varmus H. E., Capecchi M. R. Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. Cell. 1983 Jul;33(3):705–716. doi: 10.1016/0092-8674(83)90013-2. [DOI] [PubMed] [Google Scholar]
  28. Lusky M., Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981 Sep 3;293(5827):79–81. doi: 10.1038/293079a0. [DOI] [PubMed] [Google Scholar]
  29. Mark W., Sugden B. Transformation of lymphocytes by Epstein-Barr virus requires only one-fourth of the viral genome. Virology. 1982 Oct 30;122(2):431–443. doi: 10.1016/0042-6822(82)90242-2. [DOI] [PubMed] [Google Scholar]
  30. McKnight S. L., Bustin M., Miller O. L., Jr Electron microscopic analysis of chromosome metabolism in the Drosophila melanogaster embryo. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):741–754. doi: 10.1101/sqb.1978.042.01.075. [DOI] [PubMed] [Google Scholar]
  31. McKnight S. L. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res. 1980 Dec 20;8(24):5949–5964. doi: 10.1093/nar/8.24.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Mellon P., Parker V., Gluzman Y., Maniatis T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell. 1981 Dec;27(2 Pt 1):279–288. doi: 10.1016/0092-8674(81)90411-6. [DOI] [PubMed] [Google Scholar]
  33. Miyamura T., Jikuya H., Soeda E., Yoshiike K. Genomic structure of human polyoma virus JC: nucleotide sequence of the region containing replication origin and small-T-antigen gene. J Virol. 1983 Jan;45(1):73–79. doi: 10.1128/jvi.45.1.73-79.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Mocarski E. S., Roizman B. Herpesvirus-dependent amplification and inversion of cell-associated viral thymidine kinase gene flanked by viral a sequences and linked to an origin of viral DNA replication. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5626–5630. doi: 10.1073/pnas.79.18.5626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Muller W. J., Mueller C. R., Mes A. M., Hassell J. A. Polyomavirus origin for DNA replication comprises multiple genetic elements. J Virol. 1983 Sep;47(3):586–599. doi: 10.1128/jvi.47.3.586-599.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Nonoyama M., Pagano J. S. Separation of Epstein-Barr virus DNA from large chromosomal DNA in non-virus-producing cells. Nat New Biol. 1972 Aug 9;238(84):169–171. doi: 10.1038/newbio238169a0. [DOI] [PubMed] [Google Scholar]
  37. Shen Y. M., Hirschhorn R. R., Mercer W. E., Surmacz E., Tsutsui Y., Soprano K. J., Baserga R. Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency. Mol Cell Biol. 1982 Sep;2(9):1145–1154. doi: 10.1128/mcb.2.9.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Shortle D., Nathans D. Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication. J Mol Biol. 1979 Jul 15;131(4):801–817. doi: 10.1016/0022-2836(79)90202-x. [DOI] [PubMed] [Google Scholar]
  39. Soeda E., Kimura G., Miura K. Similarity of nucleotide sequences around the origin of DNA replication in mouse polyoma virus and simian virus 40. Proc Natl Acad Sci U S A. 1978 Jan;75(1):162–166. doi: 10.1073/pnas.75.1.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  41. Stow N. D., McMonagle E. C. Characterization of the TRS/IRS origin of DNA replication of herpes simplex virus type 1. Virology. 1983 Oct 30;130(2):427–438. doi: 10.1016/0042-6822(83)90097-1. [DOI] [PubMed] [Google Scholar]
  42. Sugden B. Epstein-Barr virus: a human pathogen inducing lymphoproliferation in vivo and in vitro. Rev Infect Dis. 1982 Sep-Oct;4(5):1048–1061. doi: 10.1093/clinids/4.5.1048. [DOI] [PubMed] [Google Scholar]
  43. Sugden B., Phelps M., Domoradzki J. Epstein-Barr virus DNA is amplified in transformed lymphocytes. J Virol. 1979 Sep;31(3):590–595. doi: 10.1128/jvi.31.3.590-595.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Winnacker E. L. Adenovirus DNA: structure and function of a novel replicon. Cell. 1978 Aug;14(4):761–773. doi: 10.1016/0092-8674(78)90332-x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES