Skip to main content
PMC home page
Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2010 May 27;25(2):127–132. doi: 10.1007/s12291-010-0024-z

Urinary 8-OHdG: A marker of oxidative stress to DNA and total antioxidant status in essential hypertension with South Indian population

P Subash 3, Prema Gurumurthy 3,4,✉, A Sarasabharathi 1, K M Cherian 2
PMCID: PMC3453105  PMID: 23105898

Abstract

Establishment of non-invasive urinary biomarker for the early prediction of essential hypertension (EH) is important. We evaluated whether estimation of urinary DNA, serves as a marker to predict the extent of cellular oxidative stress in essential hypertension. A total of 180 South Indian subjects aged 30–65 were recruited for the study. Of these hypertensive subjects investigated, 30 were newly diagnosed and were not on any antihypertensive drugs, but had systolic blood pressure 140–160 mmHg and diastolic blood pressure 95–100 mmHg and 75 hypertensive patients who already on drug therapy for one year and 75 were South Indian normotensive healthy controls with blood pressure ≤ 120/80 mmHg. The 8-OHdG level in urine was significantly increased in hypertensive patients (both newly diagnosed and who already on drug therapy) compared with control group. The significant increase in 8-OHdG was observed in newly diagnosed hypertensive patients compared with hypertensive patients who already on drug therapy. There was a significant decrease in serum TAS value in essential hypertensive group when compared to control group. The urinary 8-OHdG was independently correlated with serum TAS. Decreased TAS levels, which reflect to increased oxidative stress, may be the reason of increased urinary 8-OHdG in South Indian hypertensive patients. Our preliminary data suggest that the competitive ELISA for 8-OHdG appears to be a simple method for quantifying the extent of oxidative stress.

Key Words: Total Antioxidant Status, Urinary 8-OHdG, Essential Hypertension, Oxidative Stress

Full Text

The Full Text of this article is available as a PDF (230.6 KB).

References

  • 1.Chobanian A.V., Bakris G.L., Black H.R., Cushman W.C., Green L.A., Izzo J.L., Jr, et al. National Heart, Lung, and Blood Institute Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure; National High Blood Pressure Education Program Coordinating Committee. The Seventh report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. The JNC 7 report. JAMA. 2003;289:2560–2572. doi: 10.1001/jama.289.19.2560. [DOI] [PubMed] [Google Scholar]
  • 2.Cooke M.S., Evans M.D., Burd R.M., Patel K., Barnard A., Lunec J. Induction and excretion of ultraviolet-induced 8-oxo-2′-deoxyguanosine and thymine dimers in vivo: implications for PUVA. J Invest Dermatol. 2001;116:281–285. doi: 10.1046/j.1523-1747.2001.01251.x. [DOI] [PubMed] [Google Scholar]
  • 3.Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford Univ. Press 1999; 3rd ed: OX2 6DP.
  • 4.Trzeciak A.R., Nyaga S.G., Jaruga P., Lohani A., Dizdaroglu M., Evans M.K. Carcinogenesis. 2004;25:1359–1370. doi: 10.1093/carcin/bgh144. [DOI] [PubMed] [Google Scholar]
  • 5.Evans M.D., Dizdaroglu M., Cooke M.S. Oxidative DNA damage and disease: induction, repair, and significance. Mutat Res. 2004;567:1–61. doi: 10.1016/j.mrrev.2003.11.001. [DOI] [PubMed] [Google Scholar]
  • 6.Chiou C.C., Chang P.Y., Chan E.C., Wu T.L., Tsao K.C., Wu J.T. Urinary 8-hydroxy deoxy guanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder & prostate cancers. Clin Chim Acta. 2003;334:87–94. doi: 10.1016/S0009-8981(03)00191-8. [DOI] [PubMed] [Google Scholar]
  • 7.Brown R.K., McBurney A., Lunec J., Kelly F.J. Oxidative damage to DNA in patients with cystic fibrosis. Free Radic Biol Med. 1995;18:801–806. doi: 10.1016/0891-5849(94)00172-G. [DOI] [PubMed] [Google Scholar]
  • 8.Tsuboi H., Kouda K., Takeuchi H., Takigawa M., Masamoto Y., Takeuchi M., Ochi H. 8-hydoxy guanosine in urine as an index of oxidative damage to DNA in the evaluation of atopic dermatitis. Br J Dermatol. 1998;138:1033–1035. doi: 10.1046/j.1365-2133.1998.02273.x. [DOI] [PubMed] [Google Scholar]
  • 9.Rall L.C., Roubenoff R., Meydani S.N., Han S.N., Meydani M.J. Urinary 8-hydroxy -2′-deoxy guanosine (8-OHdG) as a marker of oxidative stress in rheumatoid arthritis and aging: effect of progressive resistance training. Nutr Biochem. 2000;11:581–584. doi: 10.1016/S0955-2863(00)00123-6. [DOI] [PubMed] [Google Scholar]
  • 10.Iris EF Benzie. Ferric reducing antioxidant power assay. Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods of Enzymology. 1996;299:15–27. doi: 10.1016/S0076-6879(99)99005-5. [DOI] [PubMed] [Google Scholar]
  • 11.Erdogan C. The evaluation of oxidative stress in patients with CRF. CCA. 2002;322:157–167. doi: 10.1016/s0009-8981(02)00173-0. [DOI] [PubMed] [Google Scholar]
  • 12.Iris Benzie E.F. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power. The FRAP assay. Analytical Biochem. 1996;239:70–76. doi: 10.1006/abio.1996.0292. [DOI] [PubMed] [Google Scholar]
  • 13.Azad N., Rojanasakul Y., Vallyathan V. Inflammation and lung cancer: roles of reactive oxygen/nitrogen species. J Toxicol Environ Health B Crit Rev. 2008;11:1–15. doi: 10.1080/10937400701436460. [DOI] [PubMed] [Google Scholar]
  • 14.Hwang E.S., Kim G.H. Biomarkers of Oxidative stress status of DNA, lipids and proteins in vitro and in vivo cancer research. Toxicol. 2007;229:1–10. doi: 10.1016/j.tox.2006.10.013. [DOI] [PubMed] [Google Scholar]
  • 15.Bonde P., Gao D., Chen L. Selective decrease in the DNA base excision repair pathway in squamous cell cancer of the esophagus. J Thorac Cardiovasc Surg. 2007;133:74–81. doi: 10.1016/j.jtcvs.2006.06.050. [DOI] [PubMed] [Google Scholar]
  • 16.Chen X., Ding Y.W., Yang G. Oxidative damage in an esophageal adenocarcinoma model with rats. Carcinogenesis. 2000;21:257–263. doi: 10.1093/carcin/21.2.257. [DOI] [PubMed] [Google Scholar]
  • 17.Ross R. Atherosclerosis: an inflammatory disease. N Eng J Med. 1999;340:115–126. doi: 10.1056/NEJM199901143400207. [DOI] [PubMed] [Google Scholar]
  • 18.Baynes J.W., Thorpe S.R. Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes. 1999;48:1–9. doi: 10.2337/diabetes.48.1.1. [DOI] [PubMed] [Google Scholar]
  • 19.Dandona P., Thusu K., Cook S., Snyder B., Makowski J., Armstrong D., Nicotera T. Oxidative damage to DNA in diabetes mellitus. Lancet. 1996;347:444–445. doi: 10.1016/S0140-6736(96)90013-6. [DOI] [PubMed] [Google Scholar]
  • 20.Chen L.Y., Nichols W.W., Hendricks J., Mehta J.L. Myocardial neutrophil infiltration, lipid peroxidation, and antioxidant activity after coronary artery thrombosis and thrombolysis. Am Heart J. 1995;129:211–218. doi: 10.1016/0002-8703(95)90000-4. [DOI] [PubMed] [Google Scholar]
  • 21.Honda M., Yamada Y., Tomonaga M., Ichinose H., Kamihira S. Correlation of urinary 8-hydroxy-2-de-oxyguanosine (8-OHdG), a biomarker of oxidative DNA damage and clinical features of hematological disorders: a pilot study. Leuk Res. 2000;24:461–468. doi: 10.1016/S0145-2126(00)00006-0. [DOI] [PubMed] [Google Scholar]
  • 22.Tagami M., Yamagata K., Fujino H., Nara Y., Nakagawa K., Kubota A., Numano F., Yamori Y. Genetic vulnerability of cortical neurons isolated from stroke-prone spontaneously hypertensive rats in hypoxia and oxygen reperfusion. Hypertens Res. 1999;22:23–29. doi: 10.1291/hypres.22.23. [DOI] [PubMed] [Google Scholar]
  • 23.Lacy F., Connor D., Schmid-Schenbein G. Plasma hydrogen peroxide production in hypertensives and normotensive subjects at genetic risk of hypertension. J Hypertens. 1998;16:291–303. doi: 10.1097/00004872-199816030-00006. [DOI] [PubMed] [Google Scholar]
  • 24.Russo C., Olivieri O., Girelli D., Faccini G., Zennari L.M., Lombardi S., Corrocher R. Antioxidant status and lipid peroxidation in patients with essential hypertension. J Hypertens. 1998;16:1267–1271. doi: 10.1097/00004872-199816090-00007. [DOI] [PubMed] [Google Scholar]
  • 25.Burney S., Niles J.C., Dedon P.C., Tannenbaum S.R. DNA damage in deoxynucleosides and oligonucleotides treated with peroxynitirite. Chem Res Toxicol. 1999;12:513–520. doi: 10.1021/tx980254m. [DOI] [PubMed] [Google Scholar]
  • 26.Kanuchi M., Nishioka H., Hashimoto T. Oxidative DNA damage and tubulointerstitial injury in diabetic nephropathy. Nephron. 2002;91:327–329. doi: 10.1159/000058412. [DOI] [PubMed] [Google Scholar]
  • 27.Harman S.M., Liang L., Tsitouras P., Gucciardo F., Heward C.B., Reaven P.D., Ping W., Ahmed A., Cutler R.G. Urinary excretion of three nucleic acid oxidation adducts and isoprostane F(2) alpha measured by liquid chromatography-mass spectrometry in smokers, ex-smokers and non smokers. Free Radic Biol Med. 2003;35:1301–1309. doi: 10.1016/j.freeradbiomed.2003.07.003. [DOI] [PubMed] [Google Scholar]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer

RESOURCES