Skip to main content
NCBI home page
Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2010 May 27;25(2):153–157. doi: 10.1007/s12291-010-0028-8

Evaluation of plasma hormone concentrations using Enzyme-Immunoassay/Enzyme-linked Immunosorbent assay in healthy Indian men: Effect of ethnicity

M Sachidhanandam 1, S N Singh 1, A K Salhan 1, Uday Sankar Ray 1,2,✉
PMCID: PMC3453109  PMID: 23105902

Abstract

The study involved three ethnic groups of India; Rajputs, Gorkhas and South-Indians. Each group consisted of ∼40 healthy, male soldiers between 20–50 years. The reference ranges for cortisol, testosterone, prolactin, arginine vasopressin and proAtrial natriuretic peptide1–98 were determined using Enzyme-Immunoassay (EIA) while plasma levels of thyroid-stimulating hormone, triiodothyronine, free-triiodothyronine, thyroxine and freethyroxine were measured using Enzyme-linked immunosorbent assay (ELISA). The results indicated that plasma hormone concentrations were within physiological range and inter-ethnic differences were most prominent between north- (Rajputs and Gorkhas) and south- Indians. In comparison to Radioimmunoassay, the EIA method for prolactin, thyroid-stimulating hormone, free-thyroxine gave higher values while the ELISA method for triiodothyronine, free-triiodothyronine, and thyroxine gave lower values. These differences are due to differences in assay standards and design.

Key Words: Indian population, Plasma hormones, Reference intervals, Ethnicity, EIA, ELISA

Full Text

The Full Text of this article is available as a PDF (69.8 KB).

References

  • 1.Wisdom G.B. Enzyme-Immunoassay. Clin Chem. 1976;22:1243–1255. [PubMed] [Google Scholar]
  • 2.Amballi A.A., Dada O.A., Adeleye A.O., Salu J. Evaluation of the determination of reference ranges for reproductive hormones (prolactin, FSH, LH, and testosterone) using enzyme immune assay method. Sci Res Essays. 2007;2:135–138. [Google Scholar]
  • 3.Hackney A.C., Viru A. Research Methodology: Endocrinologic measurements in Exercise Science and Sports Medicine. J Athl Train. 2008;43:631–639. doi: 10.4085/1062-6050-43.6.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.The Indian Genome Variation Consortium. The Indian Genome Variation database (IGVdb): a project overview. Hum Genet. 2005;118:1–11. doi: 10.1007/s00439-005-0009-9. [DOI] [PubMed] [Google Scholar]
  • 5.Basu M., Pal K., Prasad R., Malhotra A.S., Rao K.S., Sawhney R.C. Pituitary, gonadal and adrenal hormones after prolonged residence at extreme altitude in man. Int J Androl. 1997;20:153–158. doi: 10.1046/j.1365-2605.1997.00046.x. [DOI] [PubMed] [Google Scholar]
  • 6.Brahmachari H.D., Joseph S., Ramachandran K., Radhakrishnan U. Hormonal changes under altitude stress. Def Sci J. 1977;27:99–104. [Google Scholar]
  • 7.Anand I.S., Chandrashekhar Y., Rao K.S., Malhotra R.M., Ferrari R., Chandana J., et al. Body fluid compartments, renal blood flow, and hormones at 6,000 m in normal subjects. J Appl Physiol. 1993;74:1234–1239. doi: 10.1152/jappl.1993.74.3.1234. [DOI] [PubMed] [Google Scholar]
  • 8.Basu M., Pal K., Malhotra A.S., Prasad R., Sawhney R.C. Free and total thyroid hormones in humans at extreme altitude. Int J Biometeorol. 1995;39:17–21. doi: 10.1007/BF01320888. [DOI] [PubMed] [Google Scholar]
  • 9.Sawhney R.C., Malhotra A.S. Thyroid function in sojourners and acclimatized low landers at high altitude in man. Horm Metab Res. 1991;23:81–84. doi: 10.1055/s-2007-1003619. [DOI] [PubMed] [Google Scholar]
  • 10.Wilson J.D., Foster D.W., Kronenberg H.M., Reed Larson P. Williams Textbook of Endocinology. 10ed. USA: W.B. Saunders Company; 2003. [Google Scholar]
  • 11.Shetty P.S. Nutrition transition in India. Public Health Nutr. 2002;5:175–182. doi: 10.1079/PHN2001291. [DOI] [PubMed] [Google Scholar]
  • 12.Malati T. Whether Western normative laboratory values used for clinical diagnosis are applicable to Indian population? An Overview on reference interval. Ind J Clin Biochem. 2009;24:111–122. doi: 10.1007/s12291-009-0022-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Massi C.M., Rickett E.M., Hawkley L.C., Cacioppo J.T. Gender and ethnic differences in urinary stress hormones: the population-based Chicago health, aging, and social relations study. J Appl Physiol. 2004;97:941–947. doi: 10.1152/japplphysiol.00256.2004. [DOI] [PubMed] [Google Scholar]
  • 14.Williams D.R. Race, stress, and mental health: Findings from the Commonwealth Minority Health Survey. In: Hogue C., Hargraves M., Scott-Collins K., editors. Minority Health in America: Findings and Policy Implications from the Commonwealth Fund Minority Health Survey. Baltimore: Johns Hopkins University Press; 2000. pp. 209–243. [Google Scholar]
  • 15.Ellison P.T., Bribiescas R.G., Bentley G.R., Campbell B.C., Lipson S.F., Panter-Brick C., et al. Population variation in age-related decline in male salivary testosterone. Human Reprod. 2002;17:3251–3253. doi: 10.1093/humrep/17.12.3251. [DOI] [PubMed] [Google Scholar]
  • 16.Wang T.J., Larson M.G., Levy D., Benjamin E.J., Corey D., Leip E.P., et al. Heritability and genetic linkage of plasma natriuretic peptide levels. Circulation. 2003;108:13–16. doi: 10.1161/01.CIR.0000081657.83724.A7. [DOI] [PubMed] [Google Scholar]
  • 17.Chun T., Bankir L., Eckert G.J., Bichet D.G., Saha C., Zaidi S., et al. Ethnic differences in renal responses to Furosemide. Hypertension. 2008;52:241–248. doi: 10.1161/HYPERTENSIONAHA.108.109801. [DOI] [PubMed] [Google Scholar]
  • 18.Luft F.C., Grim C.E., Fineberg N., Weinberger M.C. Effects of volume expansion and contraction in normotensive whites, blacks, and subjects of different ages. Circulation. 1979;59:643–650. doi: 10.1161/01.cir.59.4.643. [DOI] [PubMed] [Google Scholar]
  • 19.Delang F. The disorders induced by iodine deficiency. Thyroid. 1994;4:107–128. doi: 10.1089/thy.1994.4.107. [DOI] [PubMed] [Google Scholar]
  • 20.Kochupillai N. Clinical Endocrinology in India. Curr Sci. 2000;79:1061–1067. [Google Scholar]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer

RESOURCES