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. 2009 Sep 16;24(3):294–300. doi: 10.1007/s12291-009-0055-5

Protective effect of ascorbic acid against oxidative damage induced by hydrogen peroxide in cultured human peripheral blood lymphocytes

Yasir Hasan Siddique 1,✉, Tanveer Beg 1, Mohammad Afzal 1
PMCID: PMC3453308  PMID: 23105852

Abstract

Hydrogen peroxide is one of the reactive oxygen species for cellular injury. It is overproduced during oxidative stress and is known to damage proteins, nucleic acids and cell membranes. The present study was aimed to study the protective effect of ascorbic acid against the toxic doses of hydrogen peroxide using lipid peroxidation and cytokinesis blocked micronucleus assay. Hydrogen peroxide was studied at 50, 100 and 200μM and was found to increase a dose dependent increase in lipid peroxidation and micronuclei frequency. The treatment of 100 and 200μM of hydrogen peroxide separately along with 20, 40 and 80μM of ascorbic acid showed a dose dependent decrease in the lipid peroxidation and micronuclei frequency. The results suggest a protective effect of ascorbic acid against the hydrogen peroxide induced oxidative damage in cultured human peripheral blood lymphocytes.

Key Words: Hydrogen peroxide, Ascorbic acid, Lipid peroxidation, Micronucleus, Reactive oxygen species

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