Abstract
Measurement of plasma hemoglobin is useful in variety of clinical conditions. In the present study we have developed a kinetic method to estimate plasma haemoglobin by using o-tolidine. This method is sensitive, rapid, economical, simple and less influenced by interfering substances. It measures plasma haemoglobin in the range of 6 to 400 mg/L (normal range < 50 mg/L) in less than two minutes and can be easily automated.
Key Words: Plasma hemoglobin, o-Tolidine, Kinetic method
Full Text
The Full Text of this article is available as a PDF (495.7 KB).
References
- 1.Spitz S, Maguigan WH, Dobriner K. The carcinogenic action of benzidine. Cancer 1950; 789–804. [DOI] [PubMed]
- 2.Varley H. Practical Clinical Biochemistry. 4th Ed. Delhi: CBS publishers & distributors; 1969. pp. 344–348. [Google Scholar]
- 3.Pillier-Loriette C., Wingerter P., Gerota J., Schenmetzler C. Determination of plasma hemoglobin in fresh plasma for therapeutic use by the TMB (3,3′,5,5′-tetramethyl benzidine) method. Rev Fr Transfus Immunohematol. 1983;26(3):299–312. doi: 10.1016/S0338-4535(83)80033-6. [DOI] [PubMed] [Google Scholar]
- 4.Takayanagl M., Yashiro T. Colorimetry of Hemoglobin in Plasma with 2,2′-Azino-di(3- ethylbenzthiazoline-6-sulfonic acid) (ABTS) Clin Chem. 1984;30(3):357–359. [PubMed] [Google Scholar]
- 5.Chalmers A.H., Snell L.E. Estimation of Plasma and Urinary Hemoglobin by a Rate Spectrophotometric Method. Clin Chem. 1993;39(8):1679–1682. [PubMed] [Google Scholar]
- 6.Lee K.T., Ling A.M. Determination of micro-quantities of haemoglobin in serum. Microchimica Acta. 1969;57(5):995–1002. doi: 10.1007/BF01219242. [DOI] [PubMed] [Google Scholar]
- 7.Lijana R.C., Williams M.C. Tetramethylbenzidine—a substitute for benzidine in hemoglobin analysis. J Lab Clin Med. 1979;94(2):266–276. [PubMed] [Google Scholar]
- 8.Levinson S.S., Goldman J. Measuring hemoglobin in plasma by reaction with tetramethylbenzidine. Clin Chem. 1982;28(3):471–474. [PubMed] [Google Scholar]
- 9.Fairbanks V.F., Ziesmer S.C., Brien P.C. Methods for measuring plasma hemoglobin in micromolar concentration compared. Clin Chem. 1992;38(1):132–140. [PubMed] [Google Scholar]
- 10.Kruszyna R., Smith R.P., Ou L.C. Method for measuring increased plasma hemoglobin in the presence of erythrocytes. Clin Chem. 1977;23(11):2156–2159. [PubMed] [Google Scholar]
- 11.Dacie J.V., Lewis S.M. Estimation of plasma Haemoglobin, Practical Haematology. 6th Ed. London: Churchill Livingstone; 1984. pp. 139–140. [Google Scholar]
- 12.Josephy P.D., Eling T., Mason R.P. The Horseradish Peroxidase-catalyzed Oxidation of 3,5,3′,5′-Tetramethylbenzidine. Free radical and charge-transfer complex intermediates. J Biol Chem. 1982;257(7):3669–3675. [PubMed] [Google Scholar]
- 13.Burtis C.A., Ashwood E.R., Bruns D.E., editors. Tietz textbook of clinical chemistry and molecular diagnosis. 4th ed. New Delhi: Elsevier; 2006. [Google Scholar]
- 14.Henery. Clinical chemistry-principals and techniques, Chapter 21, subsection “Urine”, 1964: P. 779–782.
- 15.Unger J., Fillipi G., Patsch W. Measurement of free haemoglobin and haemolysis index: EDTA or lithium-heparinate plasma? Clin Chem. 2007;53(9):1717–1718. doi: 10.1373/clinchem.2007.091421. [DOI] [PubMed] [Google Scholar]
- 16.Wood W.G., Kress M., Meissner D., Hanke R., Reinauer H. The Determination of free and protein-bound haemoglobin in plasma using a combination of HPLC and absorption spectrometry. Clin Lab. 2001;47:279–288. [PubMed] [Google Scholar]
- 17.Rother R.P., Bell L., Hillmen P., Gladwin M.T. The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin. A novel mechanism of human disease. JAMA. 2005;293(13):1653–1662. doi: 10.1001/jama.293.13.1653. [DOI] [PubMed] [Google Scholar]