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. 1984 Jul;81(13):3969–3972. doi: 10.1073/pnas.81.13.3969

Cross-linking of tRNA at two different sites of the elongation factor Tu.

J M Van Noort, B Kraal, L Bosch, T F La Cour, J Nyborg, B F Clark
PMCID: PMC345349  PMID: 6377304

Abstract

Recently, we reported on the induction by kirromycin of two tRNA binding sites on elongation factor Tu. To obtain independent information on the existence of these two sites and to characterize them further, 3' oxidized tRNA was cross-linked to elongation factor Tu by [3H]borohydride reduction. Specific cross-linking occurred exclusively in the presence of kirromycin. In the case of elongation factor Tu X GDP X kirromycin, cross-linking was found at lysine-208; in elongation factor Tu X GTP X kirromycin, cross-linking was at lysine-208 and lysine-237. In both elongation factor Tu complexes, kirromycin itself was found cross-linked to lysine-357. The tRNA cross-linking sites are in agreement with the idea of two different binding sites of tRNA on elongation factor Tu.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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