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. 1984 Jul;81(13):3988–3992. doi: 10.1073/pnas.81.13.3988

DNA sequences of the repressor gene and operator region of bacteriophage P2.

E Ljungquist, K Kockum, L E Bertani
PMCID: PMC345353  PMID: 6330728

Abstract

The nucleotide sequence of the repressor gene C of the temperate phage P2 has been determined. It codes for a nonbasic polypeptide, 99 amino acids long. Twelve repressor-defective mutants have been mapped. All but one are located within the presumed coding part of the gene. There is a strong promoter sequence and an 8-base-pair inverted repeat preceding the gene. The P2 repressor protein shows structural similarity to other DNA-binding proteins. The operator region for the early replication functions was located by sequencing the DNA of three virulent mutants. The sequence indicates that there are two repressor-binding sites. In addition, one of the sites shows sequence homology with part of the operator region of the biotin operon of Escherichia coli.

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Selected References

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