Abstract
In the present study the property of lectin agglutination in blood on normal and different cancer patients has been observed. The purifiedSynadenium grantii root lectin was non blood group specific and its utility as a diagnostic tool in malignancy was studied. Hemagglutination (units/ml) of red blood cells of different types of cancer was compared with the normal control's red blood cells. Out of 113 total cancer patients, only a group of 29 breast cancer patients showed significant increase in titre value (P<0.05) compared to normal control.
Key Words: Lectin, Red cell membrane, Cancer Hemagglutination
Full Text
The Full Text of this article is available as a PDF (216.5 KB).
References
- 1.Mitchell B.S., Brooks S.A., Leathem A.J., Schumacher U. Do HPA and PHA-L have the same the same binding pattern in metastasizing human breast and colon cancers? Cancer Lett. 1998;16(1):113–119. doi: 10.1016/s0304-3835(97)00414-x. [DOI] [PubMed] [Google Scholar]
- 2.Damme E.J.M., Peumans W.J., Pusztai A., Bardoez S., editors. Handbook of Plant lectins: Properties and Biomedicl Applications. Chichester, UK: John Wiley & Sons; 1998. pp. 59–80. [Google Scholar]
- 3.Mody R., Joshi S., Chaney W. Use of lectins as diagnostic and therapeutic tools for cancer. J. Pharmacol. Toxicol. Methods. 1995;33:1–10. doi: 10.1016/1056-8719(94)00052-6. [DOI] [PubMed] [Google Scholar]
- 4.Morde D.S., Nagda K.K. Erythrocyte surface changes in patients with cancer of GI tract. Pune: AFMC; 1998. pp. C–2. [Google Scholar]
- 5.Hasija K.Lectin in agglutination & its role in detection of sugars on the surface of Red Blood Cells of Normal & cancer patients Ind. J. Clin. Biochem. 1991689–96.10.1007/BF02863604 [Google Scholar]
- 6.Premratna A., Sadaksharaswamy M., Nanjappa S. Isolation & purification of lectin fromS. grantii latex. Indian J Path. Microbiol. 1981;27:91–98. [Google Scholar]
- 7.Pushpa Durgawale. Isolation & characterization of lactose & galactose specific lectin from roots ofSynadenium grantii (Hook F) Kolhapur: Shivaji University; 1995. pp. 80–80. [Google Scholar]
- 8.Marsh H. Serological and Immunological methods. 8th edn. Toronto, Canada: Canadian Red-cross Blood transfusion Service; 1981. pp. 51–51. [Google Scholar]
- 9.David C., Kilpatrick C, Yeoman M.M. Purification of the lectin from Datura stramonium. Biochem. J. 1978;175:1151–1153. doi: 10.1042/bj1751151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Jordinson M., Hariry I.E.I., Calnan D., Calam J., Pignatelli M. Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon calls. Gut. 1991;44:709–714. doi: 10.1136/gut.44.5.709. [DOI] [PMC free article] [PubMed] [Google Scholar]