Skip to main content
Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2008 Mar 6;23(1):81–84. doi: 10.1007/s12291-008-0019-1

Pleural fluid MDA and serum-effusion albumin gradient in pleural effusion

Manaswini Mangaraj 1,, S Kumari 1, R Nanda 1, M R Pattnaik 2, P C Mohapatra 1
PMCID: PMC3453668  PMID: 23105727

Abstract

Pleural fluid malondialdehyde (PMDA) and serum effusion albumin gradient(SEAG) were estimated in 60 patients of pleural effusion of diverse etiologies. The results were compared with Light’s criteria to distinguish between transudates and exudates. The mean PMDA level was 0.68±0.24nmol/ml and 1.17±0.25nmol/ml in transudates and exudates respectively showing a statistically significant (p<0.05) rise in exudates in comparison to transudates. SEAG registered a significant fall in exudates (P<0.001) when compared with transudates. PMDA revealed a positive correlation with pleural protein(r=+0.30) and a significant negative association with SEAG (r= −0.33).Sensitivity and specificity of PMDA were better than the parameters of Light’s criteria. Whereas SEAG documented approximately equal sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) with Light’s criteria. Therefore PMDA and SEAG can be taken together in addition to Light’s criteria to strengthen the discrimination between transudates and exudates in borderline cases of pleural effusion.

Key Words: Pleural Fluid, Exudates, Transudates

Full Text

The Full Text of this article is available as a PDF (104.1 KB).

References

  • 1.Light R.W., Mac Gregor I., Luelisinger P.C., Ball W.C. Pleural effusions: the diagnostic separations of transudates and exudates. Ann Intern Med. 1972;77:507–573. doi: 10.7326/0003-4819-77-4-507. [DOI] [PubMed] [Google Scholar]
  • 2.Storey D.D., Dines D.E., Coles D.T. Pleural effusions: a diagnostic dilemma. JAMA. 1976;236:2183–2186. doi: 10.1001/jama.236.19.2183. [DOI] [PubMed] [Google Scholar]
  • 3.Hinrich H., Brohan W., Bohmer R. Cholesterol in pleural effusions. Chest. 1987;92(2):296–302. doi: 10.1378/chest.92.2.296. [DOI] [PubMed] [Google Scholar]
  • 4.Hamm H., Brohan U., Bohmer R., Missmahl H.P. Cholesterol in pleural effusion; a diagnostic aid. Chest. 1987;92:296. doi: 10.1378/chest.92.2.296. [DOI] [PubMed] [Google Scholar]
  • 5.Meisel S., Shamiss A., Thaler M. Pleural fluid to serum billirubin concentration ratio for the separation of transudates from exudates. Chest. 1990;98:141. doi: 10.1378/chest.98.1.141. [DOI] [PubMed] [Google Scholar]
  • 6.Garcia-Pachon E, Navas IP, Sanchez JF, Jimenez B Custardoy J. Pleural fluid to serum choline esterase ratio for the separation of transudates and exudates. Chest 1996; 110(1): July. [DOI] [PubMed]
  • 7.Roth B.J., O’Meara T.F., Cragun W.H. The serum effusion albumin gradient in the evaluation of pleural effusion. Chest. 1990;98:546–549. doi: 10.1378/chest.98.3.546. [DOI] [PubMed] [Google Scholar]
  • 8.Dhar M.C., Chaudhary S., Basu K., et al. Serum effusion albumin gradient in the differential diagnosis of pleural effusion. Ind J Tub. 2000;18:241–245. [Google Scholar]
  • 9.Alfredo C., Luis H., Celia T. Evaluation of different criteria for the separation of pleural transudates from exudates. Chest. 1993;104(2):399. doi: 10.1378/chest.104.2.399. [DOI] [PubMed] [Google Scholar]
  • 10.Romero S., Fernadez C., Martin C. Influence of diuretics on the concentration of proteins and other components of pleural transudates in patients with heart failure. Am J Med. 2001;110:681–686. doi: 10.1016/S0002-9343(01)00726-4. [DOI] [PubMed] [Google Scholar]
  • 11.Hammouda R.M.A., Khalid M.M. Salem A Lipid peroxidation products in pleural fluid for separation of transudates and exudates. Clin Chem. 1995;41(9):1314. [PubMed] [Google Scholar]
  • 12.Gupta K.B. Evaluation of pleural fluid and MDA levels in differentiating transudative from exudative pleural effusion. Ind J Tub. 2002;49:97–100. [Google Scholar]
  • 13.Varley H.The plasma proteins: 4th edition Practical Clinical Biochemistry 1988;CBS Publishers & Distributers; 234–238.
  • 14.Doumas B.T., Watson W.A. Albumin reagent and assay. Clin Chem Acta. 1971;31:87. doi: 10.1016/0009-8981(71)90365-2. [DOI] [PubMed] [Google Scholar]
  • 15.Recommendations for the measurements of LDH in human serum at 30°C. Ann Biol Clin 1982; 40:87.
  • 16.Satoh K. Serum lipid peroxide in cerebrovascular disorder as determined by a new colorimetric method. Clin Chem Acta. 1978;90:37–43. doi: 10.1016/0009-8981(78)90081-5. [DOI] [PubMed] [Google Scholar]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer

RESOURCES