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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jul;81(13):4060–4064. doi: 10.1073/pnas.81.13.4060

Single gene for the large subunit of ribulosebisphosphate carboxylase in maize yields two differentially regulated mRNAs.

L D Crossland, S R Rodermel, L Bogorad
PMCID: PMC345368  PMID: 6330736

Abstract

A second mRNA coding for the large subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxylase (dimerizing), EC 4.1.1.39] has been found in the plastids of maize leaves. The 5' terminus of this mRNA was shown by S1 nuclease analysis to be 238 nucleotides upstream of the previously described large subunit mRNA [McIntosh, L., Poulsen, C. & Bogorad, L. (1980) Nature (London) 288, 556-560]. The same two mRNAs were produced in a homologous in vitro transcription system using cloned plastid DNA as a template. The ratio of the two mRNAs changes during light-induced plastid development and can be altered in the in vitro system by manipulation of transcription conditions.

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Selected References

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  1. Barbosa E., Moss B. mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus. Purification and physical properties. J Biol Chem. 1978 Nov 10;253(21):7692–7697. [PubMed] [Google Scholar]
  2. Bedbrook J. R., Coen D. M., Beaton A. R., Bogorad L., Rich A. Location of the single gene for the large subunit of ribulosebisphosphate carboxylase on the maize chloroplast chromosome. J Biol Chem. 1979 Feb 10;254(3):905–910. [PubMed] [Google Scholar]
  3. Coen D. M., Bedbrook J. R., Bogorad L., Rich A. Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487–5491. doi: 10.1073/pnas.74.12.5487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jolly S. O., Bogorad L. Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase. Proc Natl Acad Sci U S A. 1980 Feb;77(2):822–826. doi: 10.1073/pnas.77.2.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jolly S. O., McIntosh L., Link G., Bogorad L. Differential transcription in vivo and in vitro of two adjacent maize chloroplast genes: The large subunit of ribulosebisphosphate carboxylase and the 2.2-kilobase gene. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6821–6825. doi: 10.1073/pnas.78.11.6821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Krebbers E. T., Larrinua I. M., McIntosh L., Bogorad L. The maize chloroplast genes for the beta and epsilon subunits of the photosynthetic coupling factor CF1 are fused. Nucleic Acids Res. 1982 Aug 25;10(16):4985–5002. doi: 10.1093/nar/10.16.4985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Link G., Coen D. M., Bogorad L. Differential expression of the gene for the large subunit of ribulose bisphosphate carboxylase in maize leaf cell types. Cell. 1978 Nov;15(3):725–731. doi: 10.1016/0092-8674(78)90258-1. [DOI] [PubMed] [Google Scholar]
  8. Martin S. A., Moss B. Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions. J Biol Chem. 1975 Dec 25;250(24):9330–9335. [PubMed] [Google Scholar]
  9. Martin S. A., Moss B. mRNA guanylyltransferase and mRNA (guanine-7-)-methyltransferase from vaccinia virions. Donor and acceptor substrate specificites. J Biol Chem. 1976 Dec 10;251(23):7313–7321. [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  12. Monroy G., Spencer E., Hurwitz J. Characteristics of reactions catalyzed by purified guanylyltransferase from vaccinia virus. J Biol Chem. 1978 Jun 25;253(12):4490–4498. [PubMed] [Google Scholar]
  13. Monroy G., Spencer E., Hurwitz J. Purification of mRNA guanylyltransferase from vaccinia virions. J Biol Chem. 1978 Jun 25;253(12):4481–4489. [PubMed] [Google Scholar]
  14. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shinozaki K., Sugiura M. Sequence of the intercistronic region between the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit and coupling factor beta subunit gene. Nucleic Acids Res. 1982 Aug 25;10(16):4923–4934. doi: 10.1093/nar/10.16.4923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Steinmetz A. A., Krebbers E. T., Schwarz Z., Gubbins E. J., Bogorad L. Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions. J Biol Chem. 1983 May 10;258(9):5503–5511. [PubMed] [Google Scholar]
  17. Zurawski G., Perrot B., Bottomley W., Whitfeld P. R. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 1981 Jul 24;9(14):3251–3270. doi: 10.1093/nar/9.14.3251. [DOI] [PMC free article] [PubMed] [Google Scholar]

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