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. 2006 Mar;21(1):105. doi: 10.1007/BF02913075

Lipid peroxidation and antioxidant status in blood of rheumatoid arthritis patients

D Vijayakumar 1, K Suresh 1, S Manoharan 1,✉
PMCID: PMC3453750  PMID: 23105578

Abstract

The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects. Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis patients are more prone to free radical mediated oxidative damage.

Keywords: Rheumatoid arthritis, lipid peroxidation, antioxidants

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References

  • 1.Parke D.V., Sapota A. Chemical toxicity and reactive oxygen species. Int J Occup Med Environ Health. 1996;9:331–340. [PubMed] [Google Scholar]
  • 2.Bandyopadhyay U., Das D., Banerjee R.K. Reactive oxygen species: Oxidative damage and pathogenesis. Current Sci. 1999;77:658–666. [Google Scholar]
  • 3.Halliwell B. Free radicals, antioxidants and human disease: curiosity causes or consequences? Lancet. 1994;344:721–724. doi: 10.1016/S0140-6736(94)92211-X. [DOI] [PubMed] [Google Scholar]
  • 4.Weitzman S.A., Gorden L.I. Inflammation and cancer: role of phagocyte — generated oxidants in carcinogenesis. Blood. 1990;76:655–663. [PubMed] [Google Scholar]
  • 5.Yu B.P. Cellular defences against damage from reactive oxygen species. Biol Rev. 1994;74:139–162. doi: 10.1152/physrev.1994.74.1.139. [DOI] [PubMed] [Google Scholar]
  • 6.Ray G., Husain S.H. Oxidants, antioxidants and carcinogenesis. Indian J. Exp. Biol. 2002;40:1213–1232. [PubMed] [Google Scholar]
  • 7.Deborah P.M. Epidemiology of rheumatoid arthritis: determinants of onset, persistence and outcome. Clin. Rheum. 2002;16:707–722. doi: 10.1053/berh.2002.0257. [DOI] [PubMed] [Google Scholar]
  • 8.Aruoma O.I., Kaur H., Halliwell B. Oxygen free radicals and human diseases. J. R. Soc. Health. 1991;111:172–177. doi: 10.1177/146642409111100506. [DOI] [PubMed] [Google Scholar]
  • 9.Gambhir J.K., Lali P., Jain A.K. Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clin. Biochem. 1997;30:251–255. doi: 10.1016/S0009-9120(96)00007-0. [DOI] [PubMed] [Google Scholar]
  • 10.Taysi S., Polat F., Gul M., Sari R.a., Bakan E. Lipid peroxidation, some extracellular antioxidants, and antioxidants enzymes in serum of patients with rheumatoid arthritis. Rheumatol. Int. 2002;21:200–204. doi: 10.1007/s00296-001-0163-x. [DOI] [PubMed] [Google Scholar]
  • 11.Braven J., Ansari N., Figgitt D.P., Fisher A., Luders C., Hickling P., Whittaker M. A comparison of glutathione reductase and glutathione peroxidase activities in patients with rheumatoid arthritis and healthy adults. Br. J. Rheumatol. 1989;28:212–215. doi: 10.1093/rheumatology/28.3.212. [DOI] [PubMed] [Google Scholar]
  • 12.Dodge J.F., Mitchell G., Hanahan D.J. The preparation and chemical characterization of haemoglobin free ghosts of human red blood cells. Arch. Biochem. Bio. Phys. 1968;110:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  • 13.Quist E.H. Regulation of erythrocyte membrane shape by calcium ion. Biochem. Biophys. Res. Commun. 1980;92:631–637. doi: 10.1016/0006-291X(80)90380-0. [DOI] [PubMed] [Google Scholar]
  • 14.Yagi K. Lipid peroxides and human disease. Chem. Physiol. Lip. 1978;45:337–357. doi: 10.1016/0009-3084(87)90071-5. [DOI] [PubMed] [Google Scholar]
  • 15.Donnan S.K. The thiobarbituric acid test applied to tissues from rats treated in various ways. J. Biol. Chem. 1950;18:415–419. [Google Scholar]
  • 16.Desai F.D. Vitamin E analysis methods for animal tissues. In: Feischer S, Packer L, editors. Methods Enzymol. New York: Academic Press; 1984. pp. 138–145. [DOI] [PubMed] [Google Scholar]
  • 17.Omaye S.T., Turubull T.D., Sauberlich H.E. Selected method for the determination of ascorbic acid in animal cells, tissues and fluids. In: Cormic D, Band Wright DL, editors. Methods Enzymol. New York: Academic Press; 1979. pp. 3–11. [DOI] [PubMed] [Google Scholar]
  • 18.Beutler E., Kelley B.M. The effect of sodium nitrate on RBC glutathione. Experientia. 1963;19:96–97. doi: 10.1007/BF02148042. [DOI] [PubMed] [Google Scholar]
  • 19.Ravin H.A. An improved colorimetric enzymatic assay for ceruloplasmin. J. Lab. Clin. Med. 1961;58:161–168. [PubMed] [Google Scholar]
  • 20.Rotruck J.T., Pope A.L., Ganther H.E., Swanson A.B., Hafeman D.G., Hoekstra W.G. Selemium. Biochemical roles as a component of glutathione peroxidase. Science. 1973;179:588–590. doi: 10.1126/science.179.4073.588. [DOI] [PubMed] [Google Scholar]
  • 21.Kakkar P., Das B., Viswanathan P.N. A modified spectrophotometric assay of superoxide dimutase. Indian J. Biochem. Bio. Phys. 1984;21:130–132. [PubMed] [Google Scholar]
  • 22.Sinha K.A. Colorimetric assay of catalase. Annal. Biochem. 1972;47:389–394. doi: 10.1016/0003-2697(72)90132-7. [DOI] [PubMed] [Google Scholar]
  • 23.Ostrakhovitch E.A., Afanas’ev I.B. Oxidative stress in rheumatoid arthritis leukocytes: suppression by rutin and other antioxidants and chelators. Biochem. Pharmacol. 2001;62:743–746. doi: 10.1016/S0006-2952(01)00707-9. [DOI] [PubMed] [Google Scholar]
  • 24.Gotia S., Popovici I., Hermeziu B. Antioxidant enzymes levels in children with juvenile rheumatoid arthritis. Rev. Med. Chair. Soc. Med. Nat. Iasi. 2001;105:499–503. [PubMed] [Google Scholar]
  • 25.Gutteridge J.M.C. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem. 1995;41:1819–1828. [PubMed] [Google Scholar]
  • 26.Wagner G.M., Lubin B.H., Yechin D.T. Oxidative damage to red cells. In: Chow C.K., editor. cellular antioxidant defence mechanisms. Boca Raton: CRC Press Inc; 1998. pp. 185–195. [Google Scholar]
  • 27.Srinivas K., Bhaskar M., Aruna Kumari, R., Nagaraj K., Reddy K.K. Antioxidants, lipid peroxidation and lipoproteins in primary hypertension. Indian Heart J. 2000;52:285–285. [PubMed] [Google Scholar]
  • 28.Fairburn K., Grootveld M., Ward R.J. Alphatocopherol, lipids and lipoproteins in kneejoint synovial fluid and serum from patients with inflammatory joint disease. Clin. Sci. 1992;83:657–664. doi: 10.1042/cs0830657. [DOI] [PubMed] [Google Scholar]
  • 29.Kolanjiappan K., Manoharan S., Kayalvizhi M. Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin. Chin. Acta. 2002;326:143–149. doi: 10.1016/S0009-8981(02)00300-5. [DOI] [PubMed] [Google Scholar]
  • 30.Manoharan S., Shreeram S., Nagini S. Life style can induce lipid peroxidation and compromise of antioxidant defence mechanisms in the erythrocyte of oral cancer patients. Med. Sci. Res. 1996;24:397–400. [Google Scholar]
  • 31.Hassan M.Q., Hadi R.A., Al-Rawi Z.S., Padron V.A., Stohs S.J. The glutathione defense system in the pathogensis of rheumatoid arthritis. J. Appl. Toxicol. 2001;21:69–73. doi: 10.1002/jat.736. [DOI] [PubMed] [Google Scholar]
  • 32.Buettner G.R., Schafer F.Q. Free radicals, Oxidants and Antioxidants. Teratology. 2000;62:234–234. doi: 10.1002/1096-9926(200010)62:4<234::AID-TERA10>3.0.CO;2-9. [DOI] [PubMed] [Google Scholar]
  • 33.Kaneda H. A study on the lipid peroxide and its scavenging enzymes in rheumatoid arthritis. Nippon Seikeigeka Gakkai Zasshi. 1982;56:387–397. [PubMed] [Google Scholar]
  • 34.Cross C.E., Halliwell B., Borish E.T., Pryor W.A., Ames B.N., Saul R.L., Mccord J.M., Harman D. Oxygen radicals and human disease. Ann. Rheum. Dis. 1987;107:526–526. doi: 10.7326/0003-4819-107-4-526. [DOI] [PubMed] [Google Scholar]
  • 35.Karatas F., Ozates I., Canatan H., Halifeoglu I., Karatepe M., Colak R. Antioxidant status and lipid peroxidation in patients with rheumatoid arthritis. Indian J. Med. Res. 2003;118:178–181. [PubMed] [Google Scholar]
  • 36.Imadaya A., Teraswa K., Tosa H., Okamoto M., Toriizuka K. Erythrocyte antioxidant enzymes are reduced in patients with rheumatoid arthritis. J. Rheumatol. 1998;15:1628–1631. [PubMed] [Google Scholar]

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