Abstract
Salivary β-glucuronidase activity was studied in 20 healthy subjects without habits and 10 with habits, 38 untreated patients of advanced head and neck cancer and 21 patients after treatment either after surgery or radiation.
Salivary β-glucuronidase activity was elevated in untreated cases of head and neck cancer (p<0.001), with a fall in the activity after treatment. The post operative fall in the activity of salivary β-glucuronidase activity was highly significant (p<0.001) as compared to post radiation. This test can be utilized in the diagnosis and also for monitoring the course of the disease.
Full Text
The Full Text of this article is available as a PDF (50.7 KB).
References
- 1.Ho K.J. Urinary β glucuronidase in screening and follow up of primary urinary tract malignancy. J. Urol. 1995;154(4):1348–1350. doi: 10.1016/S0022-5347(01)66853-8. [DOI] [PubMed] [Google Scholar]
- 2.Ho K.J., Kuo S.H. Urinary β glucuronidase activity as an initial screening test for urinary tract malignancy in high risk patients: comparison with conventional urine cytological evaluation. Cancer. 1995;76(3):473–478. doi: 10.1002/1097-0142(19950801)76:3<473::AID-CNCR2820760318>3.0.CO;2-6. [DOI] [PubMed] [Google Scholar]
- 3.Karmowski A., Sobiech K.A., Majda J.M., Karmowski M., Blok K., Madej P., Malik B. Lysosomal hydrolases activity in serum and urine of patients undergoing surgery for uterine myomas III β glucuronidase (BGR) Pol. J. Gynaecol. Invest. 2003;5(3):333–338. [Google Scholar]
- 4.Glucuronidase, Beta: http://www.worthington-biochem.com/GL/default.html.
- 5.Harvey S.R., Ambaye R.Y., Panse T.B. Kinetics of β glucuronidase and its evaluation in oral cancer. Ind. J. Med. Science. 1966;20:381–387. [Google Scholar]
- 6.Szasz G. Comparison between p-Nitrophenyl glucuronide and Phenolphthalein glucuronide as substrates in the assay of β glucuronidase. Clin. Chem. 1967;13:752–759. [PubMed] [Google Scholar]
- 7.Hepler O.E. Manual of Clinical Laboratory Methods. 4th edition. Illinois, USA: Charles C Thomas publisher; 1966. pp. 275–276. [Google Scholar]
- 8.Fishman W.H., Springer B., Brunetti R. Application of an improved glucuronidase assay method to the study of human blood. J. Biol. Chem. 1948;173:449–456. [PubMed] [Google Scholar]
- 9.Khanolkar M.M., Sirsat A.V., Walvekar S.S., Bhansali M.S., Desouza L.J. Modified method for determination of serum betaglucuronidase: A comparative study using p-nitrophenyl glucuronide and phenolphthalein glucuronide as substrate in gastrointestinal tract carcinomas. Indian Journal of Clinical Biochemistry. 1997;12(1):67–70. doi: 10.1007/BF02867959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Harvey S.R., Panse T.B. Oral Cancer and β glucuronidase activity. Ind. J. Med. Res. 1960;48:692–692. [Google Scholar]