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. 2006 Mar;21(1):111–115. doi: 10.1007/BF02913077

Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR)

Ullas Kamath 1,✉, Guruprasad Rao 1, Shobha U Kamath 2, Lavanya Rai 3
PMCID: PMC3453785  PMID: 23105580

Abstract

The present study demonstrates the possibility of increased lipid peroxidation and protein oxidation in both maternal and fetal erythrocytes as markers of oxygen radical activity during intrauterine growth retardation. The erythrocyte MDA levels were significantly elevated in mothers of IUGR babies when compared to controls (p<0.01). The endogenous protein damage due to oxidative stress was significantly higher in IUGR mothers when compared to controls (p<0.05). Similarly the proteolytic activity in erythrocyte lysates against oxidatively damaged hemoglobin was significantly increased in mothers of IUGR babies compared to controls (p<0.001).

In fetuses born with IUGR, both lipid peroxidation and proteolytic activity were significantly increased when compared to normal newborns (p<0.01).

The result of this study indicates that oxidative stress was induced both in IUGR babies and their mothers which is manifested as increased lipid peroxidation and protein oxidant damage.

Key words: Intrauterine growth retardation, malodialdehyde, proteolytic activity

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References

  • 1.Frank L., Sosenko I. Failure of premature rabbits to increase antioxidant enzymes during hyperoxic exposure. Increased susceptibility to pulmonary oxygen toxicity compared with term rabbits. Pediatr. Res. 1991;29:292–296. doi: 10.1203/00006450-199103000-00014. [DOI] [PubMed] [Google Scholar]
  • 2.Frank L., Sosenko I. Development of lung antioxidant enzyme system in late gestation: Possible implications for the prematurely born infant. J. Pediatr. 1987;110:9–14. doi: 10.1016/S0022-3476(87)80279-2. [DOI] [PubMed] [Google Scholar]
  • 3.Wang W., Pang C., Rogers M., Chang A. Lipid peroxidation in cord blood at birth. Am. J. Obstet. Gynecol. 1996;174:62–65. doi: 10.1016/S0002-9378(96)70374-5. [DOI] [PubMed] [Google Scholar]
  • 4.Pitkanen O., Haliman M., Andersson S. Correlation of free oxygen radical induced lipid peroxidation with outcome in very low birth weight infants. J. Pediatr. 1990;116:760–764. doi: 10.1016/S0022-3476(05)82668-X. [DOI] [PubMed] [Google Scholar]
  • 5.Halliwell B., Gutteridge J.M.C. Free radicals in biology and medicine. 2nd ed. Oxford: Clarendon Press; 1989. [Google Scholar]
  • 6.Dizdaroglu M. Chemistry of free radical damage to DNA and nucleoproteins. In: Halliwell B, Aruoma O.I., editors. DNA and free radicals. Chichester: Ellis Harwood; 1993. pp. 19–39. [Google Scholar]
  • 7.Wolff S.P., Garner A., Dean R.T. Free radicals, lipids and protein degradation. Trends Biochem. Sci. 1986;11:27–31. doi: 10.1016/0968-0004(86)90228-8. [DOI] [Google Scholar]
  • 8.Jain S.K. Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. J. Biol. Chem. 1989;254:21340–21345. [PubMed] [Google Scholar]
  • 9.Fagan J., Waxman C., Goldberg A.C. Red blood cells contain a pathway for the degradation of oxidant damaged hemoglobin that does not require ATP or ubiquitin. J. Biol. Chem. 1986;261:5705–5713. [PubMed] [Google Scholar]
  • 10.Mudaliar and Menons clinical obstetrics, 9th Edition, 1995; 394–395.
  • 11.Jain S.K., Hochstein P. Generation of superoxide radicals by hydrazine: its role in phenylhydrazine induced hemolytic anemia. Biochim. Biophys. Acta. 1979;586:128–136. [Google Scholar]
  • 12.Riggs A. Preparations of blood hemoglobins of vertebrates. Methods Enzymol. 1981;76:5–29. doi: 10.1016/0076-6879(81)76111-1. [DOI] [PubMed] [Google Scholar]
  • 13.Raghothama C., Rao P. Increased proteolysis of oxidatively damaged hemoglobin in erythrocyte lysates in diabetes mellitus. Clin. Chim. Acta. 1994;225:65–70. doi: 10.1016/0009-8981(94)90028-0. [DOI] [PubMed] [Google Scholar]
  • 14.Peterson G.L. Determination of total protein. Methods Enzymol. 1983;91:95–119. doi: 10.1016/s0076-6879(83)91014-5. [DOI] [PubMed] [Google Scholar]
  • 15.Salvati A.M., Tentori L. Determination of aberrant hemoglobin derivatives in human blood. Methods Enzymol. 1981;76:715–731. doi: 10.1016/0076-6879(81)76153-6. [DOI] [PubMed] [Google Scholar]
  • 16.Kamath U., Rao G., Raghothama C., Rai L., Rao P. Erythrocyte indicators of oxidative stress in gestational diabetes. Acta. Paediatr. 1998;87:676–692. doi: 10.1080/080352598750014102. [DOI] [PubMed] [Google Scholar]
  • 17.Rao Guruprasad, Kamath Ullas, Raghotama C., Sujatha K., Pradeep, Rao Pragna. Maternal and fetal indicators of oxidative stress in various obstetric complications. Indian Journal of Clinical Biochemistry. 2003;18:80–86. doi: 10.1007/BF02867371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Karowicz-Bilinska A., Kowalska-Koprek U., Suzin J., Sieroszewski P. Analysis of 8-isoprostane concentration as a marker of oxidative stress in pregnant women diagnosed with IUGR. Ginekol pol. 2003;74:1137–1142. [PubMed] [Google Scholar]
  • 19.Bazowska G., Jendryczko A. Concentration of malondialdehyde (MDA) in amniotic fluid and maternal and cord serum in cases of intrauterine growth retardation. Zentralbl. Gynakol. 1994;116:329–330. [PubMed] [Google Scholar]
  • 20.Scholl T.O., Stein T.P. Oxidant damage to DNA and pregnancy outcome. J. Matern. Fetal Med. 2001;10:182–185. doi: 10.1080/714904323. [DOI] [PubMed] [Google Scholar]
  • 21.Takagi Y., Nikaido T., Toki T., Kita N., Kanai M., Ashida T., Ohira S., Konishi I. Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction. Virchows Arch. 2004;444:49–55. doi: 10.1007/s00428-003-0903-2. [DOI] [PubMed] [Google Scholar]
  • 22.Gupta P., Narang M., Banerjee B.D., Basu S. Oxidative stress in term small for gestational age neonates born to undernourished mothers: a case control study. BMC Pediatr. 2004;4:14–14. doi: 10.1186/1471-2431-4-14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Karowicz-Bilinska A., Suzin J., Sieroszewski P. Evaluation of oxidative stress indices during treatment in pregnant women with intrauterine growth retardation. Med. Sci. Monit. 2002;8:CR211–216. [PubMed] [Google Scholar]
  • 24.Wispe J.R., Bell E.F., Roberts R.J. Assessment of lipid peroxidation in newborn infants and rabbits by measurements of expired ethane and pentane: influence of parenteral infusion. Pediatr. Res. 1985;19:374–379. doi: 10.1203/00006450-198519040-00012. [DOI] [PubMed] [Google Scholar]
  • 25.Smith C.V., Hansen J.N., Martin N.E., McMicken H.W., Elliot S.J. Oxidant stress responses in premature infants during exposure to hyperoxia. Pediatr. Res. 1993;34:360–365. doi: 10.1203/00006450-199309000-00024. [DOI] [PubMed] [Google Scholar]
  • 26.Sullivan J.N., Newton A.B. Serum antioxidant activity in neonates. Arch. Dis. Child. 1988;63:748–757. doi: 10.1136/adc.63.7_Spec_No.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Sullivan J.L. Iron, plasma antioxidants and the “oxygen radical disease of prematurity”. AJDC. 1988;142:1341–1344. doi: 10.1001/archpedi.1988.02150120095048. [DOI] [PubMed] [Google Scholar]
  • 28.Karowicz-Bilinska A., Marszalek M., Kowaksla-Koprek U., Suzin J., Sieroszewski P. Plasma carbonyl group concentration in pregnant women with IUGR treated by L-arginine and acetylsalicylic acid. Ginekol. Pol. 2004;75:15–20. [PubMed] [Google Scholar]
  • 29.Karowicz-Bilinska A., Kowalska-Koprek U., Suzin J., Sieroszewski P. Total antioxidative activity measured by ABTS method in pregnant women treated with L-arginine for IUGR. Ginekol. Pol. 2003;74:1130–1136. [PubMed] [Google Scholar]

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