Abstract
The antimicrobial activity of crude and methanol extract ofTerminalia bellerica dry fruit was tested by disc diffusion method, against 9 human microbial pathogens. Crude aqueous extract of dry fruit at 4 mg concentration showed zone of inhibition ranging from 15.5–28.0 mm.S. aureus was found to be highly susceptible forming highest zone of inhibition, suggesting thatT. bellerica was strongly inhibitory towards this organism. These pathogens were highly sensitive to the methanol extract forming 14.0 to 30.0 mm zone of inhibition suggesting that the methanol extract ofT. bellerica was more effective than crude extract against most of the microbes tested exceptE. coli (enteropathogen) andP. aeruginosa. The minimal inhibitory concentrations (MICs) of crude and methanol extracts were determined by broth dilution technique which ranged from 300 to >2400 μg/ml and 250 μg to >2000 μg/ml respectively, indicating thatT. bellerica was highly effective againstS. aureus with lower MIC values. There were some biochemical alterations induced byT. bellerica. These results indicate thatT. bellerica dry fruit possesses potential broad spectrum antimicrobial activity.
Key Words: Antimicrobial activity, Biochemical alterations, Human pathogens, MIC, T. bellerica
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References
- 1.Ritch-Kro E.M., Turner N.J., Towers G.H. Carrier herbal medicine: an evaluation of the antimicrobial and anti cancer activity in some frequently used remedies. J. Ethnol. Pharmacol. 1996;5:151–156. doi: 10.1016/0378-8741(96)01407-9. [DOI] [PubMed] [Google Scholar]
- 2.Colombo M.L., Bosisio E. Pharmacological activities ofChelidonium majus L (Papaveraceae) Pharmacol, Res. 1996;33:127–134. doi: 10.1006/phrs.1996.0019. [DOI] [PubMed] [Google Scholar]
- 3.Martins A.P., Salgueiro L., Goncalves M.J., Proenca da Cunha A., Vila R., Canigueral S., Mazzoni V. Essential oil composition and antimicrobial activity of three Zingiberaceae fromS. Tome e Principe. Planta. Med. 2001;67:580–584. doi: 10.1055/s-2001-16494. [DOI] [PubMed] [Google Scholar]
- 4.Scazzocchio F., Cometa M.F., Tomassini L., Palmery M. Antibacterial activity ofHydrastis Canadensis extract and it’s major isolated alkaloids. Planta. Med. 2001;67:561–563. doi: 10.1055/s-2001-16493. [DOI] [PubMed] [Google Scholar]
- 5.Rastogi P., Mehrotra B.N. Compendium of Indian Medicinal Plants, Drug research perspective. New Delhi: CDRI Lucknow and NISCOM; 1999. pp. 1–859. [Google Scholar]
- 6.Elizabeth K.M. Antimicrobial activity ofAllium sativum on some pathogenic bacteria. Indian J. Microbiol. 2001;41:321–323. [Google Scholar]
- 7.Cappuccino, J.G. and Sherman, N. (1999) Microbology a laboratory manual. 4th Edition, Addison-Wiley Longman, Inc.
- 8.Elizabeth K.M. Antimicrobial activity ofBalanites roxburghii on certain human pathogenic microorganisms. Asian. J. Microbiol. Biotech. and env. Sci. 2002;4:515–519. [Google Scholar]
- 9.Chung K.T., Wong T.Y., Wei C.I., Huang Y.W., Lin Y. Tannins and human health: a review. Crit. Rev. Food Sci. Nutr. 1998;38:421–464. doi: 10.1080/10408699891274273. [DOI] [PubMed] [Google Scholar]
- 10.Murali Y.K., Chander Ramesh, Murthy P.S. Antyhyperglycemic effect of water extract of dry fruits ofTerminalia chebula in experimental diabetes mellitus. Ind. J. Clin. Biochem. 2004;19:202–204. doi: 10.1007/BF02894285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Chander Ramesh, Singh. Kavita, Khanna A.K., Kaul S. M., Puri. Anju, Saxena. Rashmi, Bhatia. Gitika, Rizvi Farhan, Rastogi A.K. Antidyslipidemic and antioxidant activities of different fractions ofTerminalia arjuna stem bark. Ind. J. Clin. Biochem. 2004;19:141–148. doi: 10.1007/BF02894274. [DOI] [PMC free article] [PubMed] [Google Scholar]