Abstract
The present study deals with the estimation of total carbohydrate, protein bound carbohydrate, bound fucose and sialic acid along with total protein in disease conditions like gingivitis, periodontitis and their comparison with the normals.
The neutral hexose values in gingivitis (8.08±2.20mg/100mg protein) and periodontitis (12.5±2.16mg/ 100mg protein) decreased significantly when expressed per 100 mg protein compared to normals (19.8±1.89mg/100mg protein). This might be because of higher protein concentration in these two clinical conditions. The ethanol insoluble hexose values were significantly reduced in both these conditions compared to controls (3.71±1.64,5.91±1.63,7.65±0.86mg/100mg protein respectively). The ethanol soluble hexose values were also found to be drastically reduced. This decrease in saliva appears to be characteristic feature of oral diseases. In gingivitis and periodontitis fucose level was found to be increased compared to normals when expressed as a function of salivary volume. However in terms of protein concentration the values in gingivitis (2.95±1.59), periodontitis (3.26±0.98) and normals (3.20±0.50mg/100mg) were not different. Sialic acid in ethanol insoluble fraction of salivary samples mg/100mg protein was found to be significantly reduced in both gingivitis (0.78±0.33) and periodontitis (0.95±0.31) compared to controls (1.92±0.33)
Key words: Salivary glycoproteins, fucose, sialic acid/analysis, periodontitis, gingivitis
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References
- 1.Lundquist, C. (1952) Oral Sugar clearance. Odont Revy. 3, Suppl. 1. [PubMed]
- 2.Christopher, L. B. L. (1975) Salivary glands and saliva, Chapter VII, In: Applied Physilogy of the Mouth (Ferguson, D B. Eds.). 145.
- 3.Vaes G. Cell-to-cell interactions in the secretion of enzymes of connective tissue breakdown, collagenase and proteoglycan degrading neutralproteases. Agents Action. 1980;10(6):474–474. doi: 10.1007/BF02024145. [DOI] [PubMed] [Google Scholar]
- 4.Lamster I. B., Hartley L. J., Vogel R. I. Development of a Biochemical profile for gingival crevicular fluid. Methodological considerations and evaluation of collagen degrading and ground substance degrading enzyme activity during experimental gingivitis. J. Periodontal. 1985;56:13–13. doi: 10.1902/jop.1985.56.11s.13. [DOI] [PubMed] [Google Scholar]
- 5.Arthur B.N.J., Leonard S., Louis C.F., Nelson W. Gingival fluid fucose to protein ratios as indicators of the severity of periodontal disease. J. Periodontal. 1980;51:88–88. doi: 10.1902/jop.1980.51.2.88. [DOI] [PubMed] [Google Scholar]
- 6.Smith K.P., Krohn I.R., Hermanson G.T., et al. Measurement of protein using Bicinchoninic acid. Anal Biochem. 1985;150:76–76. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
- 7.Rao P., Pattabriraman T.N. Revaluation of the Phenol-sulphuric acid reaction for the estimation of Hexoses and pentoses. Anal. Biochem. 1989;181(1):18–18. doi: 10.1016/0003-2697(89)90387-4. [DOI] [PubMed] [Google Scholar]
- 8.Pattabiraman T.N. Interaction Methylfeuraldehyde and fucose with cysteine and o-cresol in sulfuric acis medium. Biochem. Arch. 1997;13:201–201. [Google Scholar]
- 9.Yao K., Ubuka T., Masuoka N., Kinuta M., Ikeda T. Direct determination of bound sialic acid in sialoglycoproteins by acidic ninhydrin reaction. Anal. Biochem. 1989;179:332–332. doi: 10.1016/0003-2697(89)90138-3. [DOI] [PubMed] [Google Scholar]
- 10.Praveen K.S., Pattabiraman T.N. Salivary proteolytic activities in periodontitis, Gingivitis and Diabetes mellitus. I.J.C.B. 1998;13(I):46–46. doi: 10.1007/BF02873443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Dharapur M.S. Glycosylated proteins in body fluids and their clinical applications. Dharwad, India: Karnataka University; 1999. pp. 98–98. [Google Scholar]
- 12.Darwazeh A.M., Macfarlane T.W., McCuish A., Lamey P.J. Mixed salivary glucose levels and candidal carriage in patients with diabetes mellitus. J. Oral Pathol. Med. 1991;20(6):280–280. doi: 10.1111/j.1600-0714.1991.tb00928.x. [DOI] [PubMed] [Google Scholar]
- 13.Nieminen A., Norduland L., Uitto V.J. The effect of treatment on the periodontitis. J. Periodontal. 1993;64(4):297–297. doi: 10.1902/jop.1993.64.4.297. [DOI] [PubMed] [Google Scholar]
- 14.Beighton D., Smith K., Hayday H. The growth of bacteria and the production of exoglycoasidic enzymes in the dental plaque of macaque monkeys. Arch. Oral Biol. 1986;31:829–829. doi: 10.1016/0003-9969(86)90137-8. [DOI] [PubMed] [Google Scholar]
- 15.Smith K., Beighton D. The effects of the availability of diet on the levels of exoglycosidases in the supragingival plaque of macaque monkeys. J. Dent. Res. 1986;65:1349–1349. doi: 10.1177/00220345860650111401. [DOI] [PubMed] [Google Scholar]
- 16.Mandel I.D. Sialochemistry in diseases and clinical situations affecting salivary glands. Cri. Rev. Clin. Lab. Sci. 1980;12:321–321. doi: 10.3109/10408368009108733. [DOI] [PubMed] [Google Scholar]
- 17.Genco R.J., Evans R.T., Ellison S. A. Rieview of Dental Reasearch in microbiology with emphasis on periodontal disease. J. Am. Dent. Assoc. 1969;78:1016–1016. doi: 10.14219/jada.archive.1969.0162. [DOI] [PubMed] [Google Scholar]
- 18.Ellison S.A. Oral bacteria and periodontal disease. J. Dent. Res. 1970;49:198–198. doi: 10.1177/00220345700490020301. [DOI] [PubMed] [Google Scholar]
- 19.Socranksy S.S. Relationship of bacteria to the etiology of periodontal disease. J. Dent. Res. 1970;49:263–263. doi: 10.1177/00220345700490020401. [DOI] [PubMed] [Google Scholar]
- 20.Klestraup J., Theilade E. Microbes and periodontal disease. J. Clin. Periodontal. 1984;1:15–15. doi: 10.1111/j.1600-051X.1974.tb01235.x. [DOI] [PubMed] [Google Scholar]
- 21.Newman M.G., Socransky S.S., Savit E.D., Propas D.A., Crawford A. Studies of the microbiology of Periodontosis. J. Periodontol. 1976;47:373–373. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
- 22.Loe H., Theilade R., Jensen S.B. Experimental gingivitis in man. J. Periodontal. 1965;36:177–177. doi: 10.1902/jop.1965.36.3.177. [DOI] [PubMed] [Google Scholar]
- 23.Shinohara M., Ohura A., Ogata K., Inoue H., Miyata T., Yoshioka M. The relationship between the silaic acid concentrations in the serum and whole saliva in rats with naturally occurring gingivitis. Jpn. J. Pharmacol. 1994;64:61–61. doi: 10.1254/jjp.64.61. [DOI] [PubMed] [Google Scholar]