Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jul;81(13):4237–4239. doi: 10.1073/pnas.81.13.4237

Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.

M Hunter, A G Lopes, E L Boulpaep, G H Giebisch
PMCID: PMC345404  PMID: 6330754

Abstract

Recordings of single potassium channels from the apical membrane of rabbit cortical collecting tubule have been achieved using the patch-clamp technique. The conductive properties of the channel have been studied in inside-out patches. The slope conductance of the open channel is approximately equal to 90 pS. The channel is selective to potassium over sodium, with a selectivity ratio of 9:1. Decreasing the calcium concentration of the solution bathing the cytoplasmic face of the patch results in a decrease of the open-channel probability. Decreasing the calcium concentration to 10 nM or less completely inhibited channel activity. The channel is also inhibited by barium in a dose-dependent fashion.

Full text

PDF
4237

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  2. García-Díaz J. F., Nagel W., Essig A. Voltage-dependent K conductance at the apical membrane of Necturus gallbladder. Biophys J. 1983 Sep;43(3):269–278. doi: 10.1016/S0006-3495(83)84350-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Grantham J. J. The single nephron preparation--cortical collecting tubules. Yale J Biol Med. 1972 Jun-Aug;45(3-4):327–331. [PMC free article] [PubMed] [Google Scholar]
  4. Gögelein H., Van Driessche W. Noise analysis of the K+ current through the apical membrane of Necturus gallbladder. J Membr Biol. 1981;60(3):187–198. doi: 10.1007/BF01992557. [DOI] [PubMed] [Google Scholar]
  5. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  6. Koeppen B. M., Biagi B. A., Giebisch G. H. Intracellular microelectrode characterization of the rabbit cortical collecting duct. Am J Physiol. 1983 Jan;244(1):F35–F47. doi: 10.1152/ajprenal.1983.244.1.F35. [DOI] [PubMed] [Google Scholar]
  7. Latorre R., Miller C. Conduction and selectivity in potassium channels. J Membr Biol. 1983;71(1-2):11–30. doi: 10.1007/BF01870671. [DOI] [PubMed] [Google Scholar]
  8. Maruyama Y., Gallacher D. V., Petersen O. H. Voltage and Ca2+-activated K+ channel in baso-lateral acinar cell membranes of mammalian salivary glands. Nature. 1983 Apr 28;302(5911):827–829. doi: 10.1038/302827a0. [DOI] [PubMed] [Google Scholar]
  9. Moczydlowski E., Latorre R. Gating kinetics of Ca2+-activated K+ channels from rat muscle incorporated into planar lipid bilayers. Evidence for two voltage-dependent Ca2+ binding reactions. J Gen Physiol. 1983 Oct;82(4):511–542. doi: 10.1085/jgp.82.4.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Neil R. G., Boulpaep E. L. Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule. Am J Physiol. 1982 Jul;243(1):F81–F95. doi: 10.1152/ajprenal.1982.243.1.F81. [DOI] [PubMed] [Google Scholar]
  11. O'Neil R. G. Voltage-dependent interaction of barium and cesium with the potassium conductance of the cortical collecting duct apical cell membrane. J Membr Biol. 1983;74(2):165–173. doi: 10.1007/BF01870505. [DOI] [PubMed] [Google Scholar]
  12. Vergara C., Latorre R. Kinetics of Ca2+-activated K+ channels from rabbit muscle incorporated into planar bilayers. Evidence for a Ca2+ and Ba2+ blockade. J Gen Physiol. 1983 Oct;82(4):543–568. doi: 10.1085/jgp.82.4.543. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES