Abstract
Serum beta-glucuronidase activity was estimated by our modified method using two substrates phenolphthalein glucuronide and p-nitrophenyl glucoronide in 49 healthy subjects and 94 patients with gastrointestinal tract (GIT) carcinoma. The correlation coefficient using the two substrates was found to be r=0.8383. The method of Gabor Szasz was modified wherein the incubation time was decreased from 5 hours to 2 hours and the incubation temperature was increased from 25°C to 37°C. The increase in beta-glucuronidase activity in GIT carcinoma appears to be a good biochemical marker in patients with such type of carcinomas, a finding not reported in literature to-date.
The modified quantitative method used for estimation of beta-glucuronidase is reliable, accurate, simple and rapid.
Key Words: Determination of β-glucuronidase, GIT carcinomas
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References
- 1.Fishman W.H., Anlyan A.J. Comparison of the beta-glucuronidase activity of normal tumor and lymph node tissues of surgical patients. Science. 1947;106:66–67. doi: 10.1126/science.106.2742.66. [DOI] [PubMed] [Google Scholar]
- 2.Fishman W.H., Anlyan A.J. Beta-glucuronidase activity in human tissues. Cancer Research. 1947;7:808–818. [PubMed] [Google Scholar]
- 3.Rokicki M., Rokicki W., Paradysz A., Fryczkowski M. Evaluation of the usefulness of analysis of beta-glucuronidase levels in patients with superficial cancer and papilloma of the bladder. Pol. Tyg. Lek. 1992;47(24–26):528–529. [PubMed] [Google Scholar]
- 4.Taniguchi T., Kiyohashi A., Kitsuta T., Okabe H. Changes in enzyme activities recognized in lymphocytes from patients with carcinomas of the gastrointestinal tract. Cell Mol. Biol. 1985;31(4):259–264. [PubMed] [Google Scholar]
- 5.Finch P.J., Rogers K., Williams B.T. Beta glucuronidase, LDH, and LDH isoenzyme levels and screening for gastric cancer. Eur. J. Surg. Oncol. 1986;12(3):253–256. [PubMed] [Google Scholar]
- 6.Ho K-J. Urinary beta-glucuronidase in screening and follow up of primary urinary tract malignancy. J. Urol. 1995;154(4):1335–1338. doi: 10.1016/S0022-5347(01)66853-8. [DOI] [PubMed] [Google Scholar]
- 7.Fishman W.H., Springer B., Brunetti R. Application of an improved glucuronidase assay method to the study of human blood. J. Biol. Chem. 1948;173:449–456. [PubMed] [Google Scholar]
- 8.Szasz G. Comparison between p-Nitrophenyl Glucuronide and Phenolphthalein Glucuronide as substrates in the Assay of beta-glucuronidase. Clin. Chem. 1967;13:752–759. [PubMed] [Google Scholar]
- 9.Kato K., Yoshida K., Tsukamoto H., Nobunaga M., Masuya T., Sawada T. Synthesis of p-nitro phenyl beta-D-glucopyranosiduronic acid and its utilisation as substrate for assay of beta-glucuronidase activity. Chem. Pharm. Bull. (Tokyo) 1960;8:239–242. [Google Scholar]