Skip to main content
PMC home page
Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2004 Jul;19(2):184–189. doi: 10.1007/BF02894283

Effect of arogh—A polyherbal formulation on the marker enzymes in isoproterenol induced myocardial injury

S Suchalatha 1,✉, C S Shyamala Devi 1,✉
PMCID: PMC3454197  PMID: 23105482

Abstract

To study the protective role of Arogh on isoproterenol induced myocardial damage in rats. The effect of Arogh pretreatment on isoproterenol induced myocardial damage was assessed by studying the levels of lipid peroxides and changes in the activity of marker enzymes such as creatine kinase, lactate dehydrogenase and transaminases in serum and heart tissue. In isoproterenol administered rats, a significant decrease was observed in the activity of marker enzymes in the heart with a corresponding increase in their levels in serum. Lipid peroxide levels increased significantly in the serum and heart. In rats pretreated with arogh, the level of lipid peroxides and the activity of marker enzymes were maintained at near normal values. Pretreatment with Arogh offered a protective effect against isoproterenol induced myocardial damage in rats as evidenced by LDH isoenzyme patterm and histopathological studies of heart tissue.

Keywords: Arogh, isoproterenol, myocardial infarction, lipid peroxides, marker enzymes

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

References

  • 1.Anversa P., Sonnerblick E.H. Ischemic cardiomyopathy: pathophysiologic mechanisms. Prog. Cardiovasc. Dis. 1990;33:49–70. doi: 10.1016/0033-0620(90)90039-5. [DOI] [PubMed] [Google Scholar]
  • 2.Harada K., Futaka Y., Miwa A., Kaneta S., Fukushima H., Ogawa N. Effect of KRN 2391, a novel vasodilator, on various experimental anginal models in rats. Jpn. J. Pharmacol. 1993;63:35–35. doi: 10.1254/jjp.63.35. [DOI] [PubMed] [Google Scholar]
  • 3.Suchalatha S., Thirugnanasambantham P., Maheswaran E., Shyamala Devi C.S. Role of Arogh, a polyherbal formulation to mitigate oxidative stress in experimental myocardial infarction. Indian. J. Exp. Biol. 2004;42(2):224–226. [PubMed] [Google Scholar]
  • 4.King J. A routine method for the estimation of lactate dehydrogenase activity. J. Med. Lab. Techn. 1959;67:265–272. [PubMed] [Google Scholar]
  • 5.Okinaka S., Kumagai H., Ebashi E., Sugaita M., Momoi Y. Serum creatine phosphokinase activity in progressive muscular dystrophy and neuromuscular disease. Arch. Neurol. 1961;4:520–526. doi: 10.1001/archneur.1961.00450110050006. [DOI] [PubMed] [Google Scholar]
  • 6.Saxena K.K., Gupta B., Srivastava V.K., Saxena R.S., Singh R.C., Prasad D.N. Creatine kinase and aspartate transaminase in experimental model to predict the size of cardiac infarct. Indian J. Exp. Biol. 1988;26:235–236. [PubMed] [Google Scholar]
  • 7.Okhawa H., Ohishi N., Yagi K. Assay for lipid peroxides in animal tissue with thiobarbituric acid reaction. Anal. Biochem. 1979;95:351–354. doi: 10.1016/0003-2697(79)90738-3. [DOI] [PubMed] [Google Scholar]
  • 8.Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;193:265–265. [PubMed] [Google Scholar]
  • 9.Sheela Sasikumar C., Shyamala Devi C.S. Protective effect of Abana, a polyherbal formulation, on isoproterenol induced myocardial infarction in rats. Ind. J. Pharmacol. 2000;32:198–201. [Google Scholar]
  • 10.Paritha Ithayarasi A., Shyamala Devi C.S. Effect of á-tocopherol on isoproterenol induced changes in lipid and lipoprotein profile in rats. Ind. J. Pharmacol. 1997;29:399–404. [Google Scholar]
  • 11.Farber J.K., Young E.E. Accelerated phospholipid degradation in anoxic rat hepatocytes. Arch. Biochem. Biophys. 1997;211:313–320. doi: 10.1016/0003-9861(81)90459-8. [DOI] [PubMed] [Google Scholar]
  • 12.Ganguly P.K., Srivastava M.L., Bora P.S., Gupta M.P. Lactate dehydrogenase isozymes. Effect of isoproterenol induced myocardial necrosis. Indian J. Exp. Biol. 1980;18:1443–1445. [PubMed] [Google Scholar]
  • 13.Srivastava R.D., Dwivedi S., Sreenivasan K.K., Chandrasekar C.N. Cardiovascular effects of Terminalia species of plants. Indian Drugs. 1991;29(4):144–149. [Google Scholar]
  • 14.Chopra R.N., Chopra I.C., Varma B.S. Supplement to Glossary of Indian Medical Plants. New Delhi: CSIR; 1969. pp. 73–73. [Google Scholar]
  • 15.Bone K., Gupta Traditional uses, chemical constituents, antiplatelet, anti inflammatory, cardiovascular and antioxidant properties of ginger. Br. J. Phytotherapy. 1997;4(4):110–120. [Google Scholar]
  • 16.Inamadar M.C., Rajarama Rao M.S. Studies on the pharmacology ofT. Chebula. J. Sci. Industr. Res. 1962;21C:345–345. [Google Scholar]
  • 17.Mishra M., Shukla Y.N., Jain S.P., Kumar Shushil. Chemistry and pharmacology of some Hibiscus species. J. Medicinal and Aromatic plant Sciences. 1999;21(4):1169–1186. [Google Scholar]
  • 18.Gupta S.C., Bajaj U.K., Sharma V.N. Cardiovascular effects ofEclipta alba Hassk. (Bhringaraja) J. Res. Indian. Med. 1976;11(3):91–93. [Google Scholar]
  • 19.Khaksa G., Zoltaghari M.E., Dehpour A.R., Samadian T. Anti inflammatory and antinociceptive activity of disodium glycyrhetinic acid and hemipthalate. Planta Medica. 1996;62(4):326–328. doi: 10.1055/s-2006-957894. [DOI] [PubMed] [Google Scholar]
  • 20.Takagi, N. and Sanashiro (1996) Health foods containing antioxidative and antiallergy food materials. Japan Kokoi Tokkyo Koho. 070, 1000.
  • 21.Alam M.I., Gomes A. Viper venom induced inflammation and inhibition of free radical formation by pure compound (2-hydroxy-4 methoxy benzoic acid) isolated and purified fromH.indicus root extracts. Toxicon. 1998;36(1):207–215. doi: 10.1016/S0041-0101(97)00070-6. [DOI] [PubMed] [Google Scholar]
  • 22.Aeshbaech R., Loliger J., Scott B.C, Murcia A., Butters J., Halliwell B., Arouma O.I. Antioxidant activities of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Fd. Chem. Toxic. 1994;32(1):31–36. doi: 10.1016/0278-6915(84)90033-4. [DOI] [PubMed] [Google Scholar]
  • 23.Chandra T., Sadique J., Somasundaram S. Anti inflammatory activity ofE. alba. Fitoterapia. 1987;58(1):28–28. [Google Scholar]
  • 24.Sreeramamurthy T, Ganga Rao B, Satyanarayana T., Krishna Rao R.V. Hepatoprotective activity ofEclipta alba. Jour. Res. Edu. Ind. Med. 1993;2:41–43. [Google Scholar]
  • 25.Kim N.J., Hong N.D. Studies on the processing of crude drugs (v) on the constituents and biological activities of glycyrhiza. Korean. J. Pharmacog. 1996;27(3):196–206. [Google Scholar]
  • 26.Ballo J.M., Messer Lactate dehydrogenase isoenzymes in human hearts having decreased oxygen supply. Biochem. Biophys. Res. Commun. 1968;33:487–491. doi: 10.1016/0006-291X(68)90601-3. [DOI] [PubMed] [Google Scholar]
  • 27.Dawson D.M., Goodfriend T.L., Kaplan N.O. Lactate dehydrogenases functions of the two types. Science. 1964;143:929–933. doi: 10.1126/science.143.3609.929. [DOI] [PubMed] [Google Scholar]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer

RESOURCES