Abstract
Ocimum sanctum leaves have been traditionally used in treatment of diabetes mellitus. Dietary supplementation of fresh tulsi leaves in a dose of 2 gm/kg BW for 30 days led to significant lowering of blood glucose levels in test group. Intake ofOcimum sanctum also led to significant increase in levels of superoxide dismutase, reduced glutathione and total thiols, but marked reduction in peroxiodised lipid levels as compared to untreated control group. The leaves were found to possess both superoxide and hydroxyl free radical scavenging action. The present observations establish the efficacy ofOcimum sanctum leaves in lowering blood glucose levels and antioxidant property appears to be predominantly responsible for hypoglycemic effect.
Key Words: Ocimum sanctum, hypoglycemic effect, antioxidant, free radical scavenger
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References
- 1.Kritikar, K. R. and Basu, B. D. (1991) In:Indian medicinal plants. Edited by Bratter, E., Carris, J. F. and Bhaskar, K. S. Vol. 3, (Periodical Experts Book Agency). 1955.
- 2.Satyavati, C. R. V., Raina, M. K. and Sharma, M. (1976)Medicinal plants of India. Vol. 1 (ICMR New Delhi).
- 3.Nadkarani, A. K. (1954)Nadkarni's Indian Materia Medica. Nadkarmi, A. K. Ed. Bombay Popular Book Depot, 868.
- 4.Sarkar A., Lavania S. C., Pandey D. N., Pant M. C. Changes in the blood lipid profile after administration onOcimum sanctum (Tulsi) leaves in the normal albino rabbits. Indian J. Physiol. Pharmacol. 1994;38(4):311–312. [PubMed] [Google Scholar]
- 5.Chattopadhyay R. R. Hypoglycemic effect ofOcimum sanctum leaf extract in normal and streptozotocin diabetic rats. Ind. J. Exp. Biol. 1993;31:891–893. [PubMed] [Google Scholar]
- 6.Aggarwal P., Rai V., Singh R. B. Randomized placebo—controlled single blind trial of holy basif leaves in patients with non insulin-dependent diabetes mellitus. Int. J. Clin. Pharmacol. Ther. 1996;34:406–409. [PubMed] [Google Scholar]
- 7.UmaDevi P., Ganasoundri A. Modulation of glutathione and antioxidant enzymes byOcimum sanctum and its role in protection against radiation injury. Indian J. Exp. Biol. 1999;37:262–268. [PubMed] [Google Scholar]
- 8.Kumar R., Seth R. K., Lekhon M. S., Bhargava J. S. Serum lipid peroxide and other enzyme levels of patients suffering from thermal injury. Burn. 1995;21:96–97. doi: 10.1016/0305-4179(95)92131-U. [DOI] [PubMed] [Google Scholar]
- 9.Beuler E., Duron O., Kelly B. M. Improved method, for determination of blood glutathione. J. Lab. Clin. Med. 1963;61:882–888. [PubMed] [Google Scholar]
- 10.Misra H. P., Fridovioh I. The role of superoxide anions in auto oxidation of epinephrine and a simple assay for superoxide dismutase. J. Biochem. 1972;247:3170–3175. [PubMed] [Google Scholar]
- 11.Eluman G. L. Tissue sulfhydryl groups. Arch. Biochemical Biophys. 1959;82:70–77. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
- 12.Bhishagratua, K. K. (1991) Sootrasthanom (chapter 15, 32, 33) In:Sushrutasamhita Varanasi, Sanskrit Series, 135–137.
- 13.Sarkar A., Pant M. C. A Comparative study of the hypoglycemic action of the seeds and fresh leaves ofOcimum sanctum (Tulsi) Indian J. Physiol. Pharmacol. 1989;33:197–198. [PubMed] [Google Scholar]
- 14.Giri J. P., Suganthi B., Meera G. Effect of Tulsi (O. S) on diabetes mellitus. Ind. J. Nutr. Dietet. 1987;24:337–341. [Google Scholar]
- 15.Mani U. V., Rai V., Iyer U. Effect of Tulsi (Ocimum sanctum) leaf powder supplementation on blood sugar levels, serum lipids and tissue lipids in diabetic rats. Plant Foods Hum. Nutr. 1997;50:9–16. doi: 10.1007/BF02436038. [DOI] [PubMed] [Google Scholar]
- 16.Anderson J. W. The role of dietary carbohydrate and fiber in control of diabetes. Adv. Intern. Med. 1980;26:67–96. [PubMed] [Google Scholar]
- 17.Bradley A. A., Nathan C. F. Glutathione metabolism as a determinant of therapeutic effciency. Cancer Res. 1984;44:4224–4232. [PubMed] [Google Scholar]
- 18.Kusamran W. R., Ratanavila A., Tepsuwan A. Effects of neem flowers. Thai and Chinese bitter gourd fruits and sweet basil leaves on hepatic monooxygenases and glutathione-S transferase activities, andin vitro metabolic activation of chemical carcinogens in rats. Food Chem. Toxicol. 1998;36:475–484. doi: 10.1016/S0278-6915(98)00011-8. [DOI] [PubMed] [Google Scholar]
- 19.Panda S., Kar A. Ocimum sanctum leaf extract in the regulation of thyroid function in the male mouse. Pharmacol. Res. 1998;38(2):107–110. doi: 10.1006/phrs.1998.0338. [DOI] [PubMed] [Google Scholar]
- 20.Heanen G., Vermculen N., Timmerman H., Best A. Effect of thiols on lipid peroxidation in rat liver microsomes. Chem. Biol. Interact. 1989;31:207–212. doi: 10.1016/0009-2797(89)90035-5. [DOI] [PubMed] [Google Scholar]