Abstract
Serum adenosine deaminase (ADA), 5′ nucleotidase (5′NT) and malondialdehyde (MDA) were estimated in patients with acute infective hepatitis (AIH) along with the routine parameters of liver disease. Present study is done to evaluate these special parameters in patients with clinical history of AIH and to assess the utility of these parameters as diagnostic/ prognostic indices of liver function and to correlate special parameters with routine live function tests (LFT). ADA, 5′NT and MDA along with routine LFT was estimated in 25 patients with AIH and 25 samples from healthy voluntary blood donars served as the control group. Routine LFT was estimated by standard clinical chemistry procedures on dade behring analyser and ADA, 5′NT and MDA were estimated by berthlot reaction, fiske and subbarao method and thiobarbituric acid method respectively.
Statistical analysis showed that serum ADA, 5′NT and MDA were significantly higher in patients as compared with the controls. There was a significant positive correlation between ADA and total bilirubin and MDA and total bilirubin. Hence we can conclude that these tests would be more sensitive to diagnose the patients with AIH and that the raised bilirubin levels could be looked upon, as a protective mechanism which the liver has evolved in order to combat oxidative stress.
Key Words: ADA, 5′NT, MDA, AIH
Full Text
The Full Text of this article is available as a PDF (286.6 KB).
References
- 1.Franco R., Casada V. Cell surface adenosine deaminase much more than an ectoenzyme. Prog. Neurobiol. 1997;52(4):283–294. doi: 10.1016/S0301-0082(97)00013-0. [DOI] [PubMed] [Google Scholar]
- 2.Young I.I. Serum 5′NT: Characterization and evaluation in disease states. Ann. N. Y. Acad. Sci. 1958;75:357–357. doi: 10.1111/j.1749-6632.1958.tb36883.x. [DOI] [PubMed] [Google Scholar]
- 3.Bardawill C., Chang C. Serum Lactate dehydrogenase, Leucine aminopeptidase and 5′NT activities: Observations in patients with carcinoma of the pancrease and hepatobiliary disease. Can. Med. Assoc. J. 1963;89:755–755. [PMC free article] [PubMed] [Google Scholar]
- 4.Hill P.G., Sammons H.G. An assesment of 5′NT as a Liver function test. Q. J. Med. 1967;36:457–457. [PubMed] [Google Scholar]
- 5.Kowlessar O.D. Comparitive study of serum Leucine aminopeptidase, 5′NT and non specific alkaline phosphatase in diseases affecting the pancrease, hepatobiliary tree and bone. Am. J. Med. 1961;31:231–231. doi: 10.1016/0002-9343(61)90111-5. [DOI] [PubMed] [Google Scholar]
- 6.Maestro R.F. An approach to free radicals in Medicine and biology. Acta physiol scand (Suppl) 1980;492:153–168. [PubMed] [Google Scholar]
- 7.Cross C., Halliwell B., Borish E.T. Oxygen radicals in human disease (Davis Conference) Ann. Int. Med. 1987;107:526–545. doi: 10.7326/0003-4819-107-4-526. [DOI] [PubMed] [Google Scholar]
- 8.Southern P.A., Powis G. Free radicals in Medicine II. Involvement in human disease. Mayo. Clin. Proc. 1988;63:390–408. doi: 10.1016/s0025-6196(12)64862-9. [DOI] [PubMed] [Google Scholar]
- 9.Anand C.V., Anand Usha, Sadashivudu B. Acute effects of ethanol on production and disposal of adenosine from rat myocardium. Bio. Chem. Int. 1985;10(2):311–317. [PubMed] [Google Scholar]
- 10.Chaney A.L., Marbach E.P. For ammonia estimation. Clin. Chem. 1962;8:130–130. [PubMed] [Google Scholar]
- 11.Varley Harold. Practical Clinical Biochemistry. 4th edn. Delhi, India: CBS publishers and distributors; 1988. pp. 446–447. [Google Scholar]
- 12.Wilbur K.M., Bernheim F., Shapiro W.O. The Thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents. Arch. Biochem. Biophy. 1943;24:305–313. [PubMed] [Google Scholar]
- 13.Marilena G. New Physiological importance of classic residual product-Bilirubin. Biochemical and Molecular Medicine. 1997;61:136–142. doi: 10.1006/bmme.1997.2610. [DOI] [PubMed] [Google Scholar]