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. 2007 Mar;22(1):22–28. doi: 10.1007/BF02912876

Cardioprotective response to chronic administration of vitamin E in isoproterenol induced myocardial necrosis: Hemodynamic, biochemical and ultrastructural studies

Mukesh Nandave 1, Ipseeta Mohanty 1, T C Nag 2, Shreesh Kumar Ojha 1, Rajan Mittal 1, Santosh Kumari 3, Dharamvir Singh Arya 1,✉
PMCID: PMC3454252  PMID: 23105647

Abstract

The present study evaluated the cardioprotective potential of vitamin-E by studying its effect on hemodynamic parameters, lipid peroxidation, myocyte injury marker and ultrastructural changes in model of isoproterenol-induced myocardial necrosis in rats. Wistar albino male rats (150–200 g) were randomly divided into saline, ISP control, and vit E groups. Vitamin E group was administered vitamin E at a dose of 100mg/kg/day while saline and ISP control groups received saline orally for one month. On 29th and 30th day, ISP (85 mg/kg, sc) was administered at an interval of 24 h to vit E and ISP control rats. On 31st day, rats of all groups were anesthetized and hemodynamic parameters were recorded. At the end of experimentation, animals were sacrificed; hearts were excised and processed for biochemical and ultrastructural studies. ISP administration produced marked cardiac necrosis as evidenced by significant decrease in my ocardial creatine kinase-MB as well as increase in malonaldialdehyde levels. ISP-induced myocardial necrosis resulted in myocardial dysfunction as evidenced by significant depression in heart rate and mean arterial pressure in the ISP control group as compared to saline control. Salient ultrastructural changes including extensive loss of myofibrils, muscle necrosis, loss of mitochondria, and formation of several intracytoplasmic vacuoles and lipid droplets further confirmed the ISP-induced myocardial damage. However, subsequent to ISP challenge, vit E treatment significantly preserved the myocardium by restoring myocardial CK-MB activity, inhibiting the ISP-induced lipid peroxidation and ultrastructural changes. Additionally, pre-and co-treatment of vit E prevented the deleterious ultrastructural changes caused by ISP. These beneficial effects of chronic vit E treatment also translated into significant restoration of the altered hemodynamic parameters. The present study clearly demonstrated the cardioprotective potential of vit E at dose of 100 mg/kg in ISP-induced model of myocardial necrosis in rats. The significant restoration of altered hemodynamic parameters, myocardial CK-MB activity, prevention of ISP-induced rise in lipid peroxidation and ultrastructural changes may confirm its cardioprotective effect.

Key words: Vitamin E, Isoproterenol, Myocardial necrosis, Rats

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References

  • 1.Lonn EM, Yusuf S. Is there a role for antioxidant vitamins in the prevention of cardiovascular diseases? An update on epidemiological and clinical trials data. Can J Cardiol. 1997;13:957–65. [PubMed] [Google Scholar]
  • 2.Munteanu A, Zingg JM, Azzi A. Anti-atherosclerotic effects of vitamin E-myth or reality. J Cell Mol Med. 2004;8:59–76. doi: 10.1111/j.1582-4934.2004.tb00260.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Drevon CA. Absorption, transport and metab olism of vitamin E. Free Radic Res Commun. 1991;14:229–46. doi: 10.3109/10715769109088952. [DOI] [PubMed] [Google Scholar]
  • 4.Saldeen T, Li D, Mehta JL. Different effects of alpha-and gamma-tocopherol on LDL oxidation, superoxide activity, platelet aggregation and arte rial thrombogenesis. J Am Coll Cardiol. 1999;34:1208–15. doi: 10.1016/S0735-1097(99)00333-2. [DOI] [PubMed] [Google Scholar]
  • 5.Li D, Saldeen T, Romeo F, Mehta JL. Relative effects of alpha-and gamma-tocopherol on LDL oxidation, superoxide dismutase and nitric oxide synthase activity and protein expression in rats. J Cardiovasc Pharmacol Ther. 1999;4:219–26. doi: 10.1177/107424849900400403. [DOI] [PubMed] [Google Scholar]
  • 6.Mohanty I, Arya DS, Dinda A, Talwar KK, Joshi S, Gupta SK. Mechanisms of cardioprotective effect ofWithania somnifera in experimentally induced myocardial infarction. Basic Clin Pharmacol Toxicol. 2004;94:184–90. doi: 10.1111/j.1742-7843.2004.pto940405.x. [DOI] [PubMed] [Google Scholar]
  • 7.Sabeena Farvin KH, Anandan R, Kumar SH, Shiny KS, Sankar TV, Thankappan TK. Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats. Pharmacol Res. 2004;50:231–6. doi: 10.1016/j.phrs.2004.03.004. [DOI] [PubMed] [Google Scholar]
  • 8.Noronha-Dutra AA, Steen EM, Woolf N. The correlation between catecholamine and lipid peroxidation induced in heart cells. Basic Res Cardiol. 1984;80:133–6. doi: 10.1007/978-3-662-11041-6_26. [DOI] [PubMed] [Google Scholar]
  • 9.Tappia PS, Heta T, Dhalla NS. Role of oxidative stressing catecholamines-induced changes in cardiac sarcolemmal calcium transport. Arch Biochem Biophy. 2001;377:85–92. doi: 10.1006/abbi.2000.2234. [DOI] [PubMed] [Google Scholar]
  • 10.Bandyopadhyay D, Chattopadhyay A, Ghosh G, Datta AG. Oxidative stress-induced ischemic heart disease: protection by antioxidants. Curr Med Chem. 2004;11:369–87. doi: 10.2174/0929867043456016. [DOI] [PubMed] [Google Scholar]
  • 11.Young YS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001;54:176–86. doi: 10.1136/jcp.54.3.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Sharma M, Kishore K, Gupta SK, Joshi S, Arya DS. Cardioprotective potential ofocimum sanctum in isoproterenol induced myocardial infarction in rats. Mol Cell Biochem. 2001;225:75–83. doi: 10.1023/A:1012220908636. [DOI] [PubMed] [Google Scholar]
  • 13.Grimm D, Elsner D, Schunkert H, Pfeifer M, Griese D, Bruckschlegel G, Muders F, Reigger GA, Kromer EP. Development of heart failure following isoproterenol administration in the rat: role of the renin angiotensin system. Cardiovasc Res. 1998;37:91–100. doi: 10.1016/S0008-6363(97)00212-5. [DOI] [PubMed] [Google Scholar]
  • 14.Bagchi D, Garg A, Krohn RL, Bagchi M, Bagchi DJ, Balmoori J, Stohs SJ. Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice. Gen Pharmacol. 1998;30:771–6. doi: 10.1016/S0306-3623(97)00332-7. [DOI] [PubMed] [Google Scholar]
  • 15.Ohkawa H, Ohishi N, Yagi K. Assay of lipid pe roxide in animal tissue by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–8. doi: 10.1016/0003-2697(79)90738-3. [DOI] [PubMed] [Google Scholar]
  • 16.Lamprecht W, Stan F, Weisser H, Heinz F. Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In: Bergmeyer HU, editor. Methods of Enzymatic Analysis. New York: Academic Press; 1974. pp. 1776–8. [Google Scholar]
  • 17.Rona G, Chappel CI, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Arch Path. 1959;76:443–5. [PubMed] [Google Scholar]
  • 18.Nirmala C, Puvanakrishnan R. Isoproterenol induced myocardial infarction in rats: functional and biochemical alterations. Med Sci Res. 1994;22:575–7. [Google Scholar]
  • 19.Haenen GR, Veerman M, Bast A. Reduction of betaadrenoreceptor function by oxidative stress in the heart. Free Radic Biol Med. 1990;9:279–88. doi: 10.1016/0891-5849(90)90002-Z. [DOI] [PubMed] [Google Scholar]
  • 20.Singal PK, Kapur N, Dhillon KS, Beamish RE, Dhalla NS. Role of free radicals in catecholamine induced cardiomyopathy. Can J Physiol Pharmacol. 1982;60:1390–97. doi: 10.1139/y82-207. [DOI] [PubMed] [Google Scholar]
  • 21.Sevenian A, Hochstein P. Mechanism and consequences of lipid peroxidation in biological systems. Ann Rev Nutr. 1985;5:365–75. doi: 10.1146/annurev.nu.05.070185.002053. [DOI] [PubMed] [Google Scholar]
  • 22.Singal PK, Beamish RE, Dhalla NS. Potential oxidative pathways of catecholamines in the formation of lipid peroxides and genesis of heart disease. Adv Exp Med Biol. 1983;61:391–401. doi: 10.1007/978-1-4684-4472-8_22. [DOI] [PubMed] [Google Scholar]
  • 23.Mair J, Wagner I, Jakob G, Lechleitner P, Drenstl F, Paschenctort B, Michel G. Different time courses of cardiac contractile proteins after acute myocardial infarction. Clin Chim Acta. 1994;231:47–60. doi: 10.1016/0009-8981(94)90253-4. [DOI] [PubMed] [Google Scholar]

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