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. 1984 Apr;81(7):2218–2222. doi: 10.1073/pnas.81.7.2218

Birth of rhesus monkey infant after in vitro fertilization and nonsurgical embryo transfer.

B D Bavister, D E Boatman, K Collins, D J Dierschke, S G Eisele
PMCID: PMC345469  PMID: 6326113

Abstract

The birth of a rhesus monkey resulting from in vitro fertilization is reported. Oocytes recovered at laparoscopy from five gonadotropin-stimulated donors were inseminated in vitro with sperm preincubated with caffeine and dibutyryl cyclic AMP. After insemination, oocytes were cultured for 33-46 hr. Twenty-two embryos were transferred nonsurgically into 11 recipient females. One recipient showed signs of implantation but did not carry to term. A second female became pregnant after receiving one 4-cell and one 6-cell embryo fertilized in vitro. The subsequent course of early pregnancy and embryonic and fetal development were characteristic of normal singleton pregnancies. A healthy term male infant was delivered by Cesarean section 176 days after fertilization. This birth has validated our procedures for in vitro fertilization of rhesus monkey gametes and provides an experimental model for studies of early embryonic development in primates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aitken R. J., Best F., Richardson D. W., Schats R., Simm G. Influence of caffeine on movement characteristics, fertilizing capacity and ability to penetrate cervical mucus of human spermatozoa. J Reprod Fertil. 1983 Jan;67(1):19–27. doi: 10.1530/jrf.0.0670019. [DOI] [PubMed] [Google Scholar]
  2. Atkinson L. E., Hotchkiss J., Fritz G. R., Surve A. H., Neill J. D., Knobil E. Circulating levels of steroids and chorionic gonadotropin during pregnancy in the rhesus monkey, with special attention to the rescue of the corpus luteum in early pregnancy. Biol Reprod. 1975 Apr;12(3):335–345. doi: 10.1095/biolreprod12.3.335. [DOI] [PubMed] [Google Scholar]
  3. Batta S. K., Stark R. A., Brackett B. G. Ovulation induction by gonadotropin and prostaglandin treatments of rhesus monkeys and observations of the ova. Biol Reprod. 1978 Mar;18(2):264–278. doi: 10.1095/biolreprod18.2.264. [DOI] [PubMed] [Google Scholar]
  4. Bavister B. D., Boatman D. E., Leibfried L., Loose M., Vernon M. W. Fertilization and cleavage of rhesus monkey oocytes in vitro. Biol Reprod. 1983 May;28(4):983–999. doi: 10.1095/biolreprod28.4.983. [DOI] [PubMed] [Google Scholar]
  5. Bavister B. D., Yanagimachi The effects of sperm extracts and energy sources on the motility and acrosome reaction of hamster spermatozoa in vitro. Biol Reprod. 1977 Mar;16(2):228–237. doi: 10.1095/biolreprod16.2.228. [DOI] [PubMed] [Google Scholar]
  6. Bennett J. P. The induction of ovulation in the squirrel monkey (Saimiri sciureus) with pregnant mares serum (PMS) and human chorionic gonadotrophin (HCG). J Reprod Fertil. 1967 Apr;13(2):357–359. doi: 10.1530/jrf.0.0130357. [DOI] [PubMed] [Google Scholar]
  7. Bielert C., Czaja J. A., Eisele S., Scheffler G., Robinson J. A., Goy R. W. Mating in the rhesus monkey (Macaca mulatta) after conception and its relationship to oestradiol and progesterone levels throughout pregnancy. J Reprod Fertil. 1976 Jan;46(1):179–187. doi: 10.1530/jrf.0.0460179. [DOI] [PubMed] [Google Scholar]
  8. Brackett B. G., Bousquet D., Boice M. L., Donawick W. J., Evans J. F., Dressel M. A. Normal development following in vitro fertilization in the cow. Biol Reprod. 1982 Aug;27(1):147–158. doi: 10.1095/biolreprod27.1.147. [DOI] [PubMed] [Google Scholar]
  9. CHANG M. C. Fertilization of rabbit ova in vitro. Nature. 1959 Aug 8;184(Suppl 7):466–467. doi: 10.1038/184466a0. [DOI] [PubMed] [Google Scholar]
  10. Czaja J. A., Eisele S. G., Goy R. W. Cyclical changes in the sexual skin of female rhesus: relationships to mating behavior and sucessful artificial insemination. Fed Proc. 1975 Jul;34(8):1680–1684. [PubMed] [Google Scholar]
  11. Czaja J. A., Robinson J. A., Eisele S. G., Scheffler G., Goy R. W. Relationship between sexual skin colour of female rhesus monkeys and midcycle plasma levels of oestradiol and progesterone. J Reprod Fertil. 1977 Jan;49(1):147–150. doi: 10.1530/jrf.0.0490147. [DOI] [PubMed] [Google Scholar]
  12. DeMayo F. J., Mizoguchi H., Dukelow W. R. Fertilization of squirrel monkey and hamster ova in the rabbit oviduct (xenogenous fertilization). Science. 1980 Jun 27;208(4451):1468–1469. doi: 10.1126/science.6770463. [DOI] [PubMed] [Google Scholar]
  13. Gould K. G., Cline E. M., Williams W. L. Observations on the induction of ovulation and fertilization in vitro in the squirrel monkey (Saimiri sciureus). Fertil Steril. 1973 Apr;24(4):260–268. [PubMed] [Google Scholar]
  14. Gould K. G. Ovum recovery and in vitro fertilization in the chimpanzee. Fertil Steril. 1983 Sep;40(3):378–383. doi: 10.1016/s0015-0282(16)47304-8. [DOI] [PubMed] [Google Scholar]
  15. Hodgen G. D., Tullner W. W., Vaitukaitis J. L., Ward D. N., Ross G. T. Specific radioimmunoassay of chorionic gonadotropin during implantation in rhesus monkeys. J Clin Endocrinol Metab. 1974 Sep;39(3):457–464. doi: 10.1210/jcem-39-3-457. [DOI] [PubMed] [Google Scholar]
  16. Hotchkiss J., Atkinson L. E., Knobil E. Time course of serum estrogen and luteinizing hormone (LH) concentrations during the menstrual cycle of the rhesus monkey. Endocrinology. 1971 Jul;89(1):177–183. doi: 10.1210/endo-89-1-177. [DOI] [PubMed] [Google Scholar]
  17. Jainudeen M. R., Hafez E. S. Gonadotrophin-induced multiple ovulation in the crab-eating monkey, Macaca fascicularis. J Reprod Fertil. 1973 Apr;33(1):151–154. doi: 10.1530/jrf.0.0330151. [DOI] [PubMed] [Google Scholar]
  18. Katzberg A. A., Hendrickx A. G. Gonadotropin-induced anomalies of the zona pellucida of the baboon ovum. Science. 1966 Mar 11;151(3715):1225–1226. doi: 10.1126/science.151.3715.1225. [DOI] [PubMed] [Google Scholar]
  19. Kreitmann O., Hodgen G. D. Retarded cleavage rates of preimplantation monkey embryos in vitro. JAMA. 1981 Aug 7;246(6):627–629. [PubMed] [Google Scholar]
  20. Lopata A. Concepts in human in vitro fertilization and embryo transfer. Fertil Steril. 1983 Sep;40(3):289–301. doi: 10.1016/s0015-0282(16)47289-4. [DOI] [PubMed] [Google Scholar]
  21. Marston J. H., Penn R., Sivelle P. C. Successful autotransfer of tubal eggs in the rhesus monkey (Macaca mulatta). J Reprod Fertil. 1977 Jan;49(1):175–176. doi: 10.1530/jrf.0.0490175. [DOI] [PubMed] [Google Scholar]
  22. Rowson L. E., Lawson R. A., Moor R. M., Baker A. A. Egg transfer in the cow: synchronization requirements. J Reprod Fertil. 1972 Mar;28(3):427–431. doi: 10.1530/jrf.0.0280427. [DOI] [PubMed] [Google Scholar]
  23. Sullivan P. T., Blystad C., Stone W. H. Immunogenetic studies of rhesus monkeys. VII. A simple hemagglutination technique for blood typing. J Immunol Methods. 1977;14(1):31–36. doi: 10.1016/s0022-1759(97)90018-1. [DOI] [PubMed] [Google Scholar]
  24. Sullivan P. T., Blystad C., Stone W. H. Immunogenetic studies on the rhesus monkey (Macaca mulatta): XI use of blood groups in problems of parentage. Lab Anim Sci. 1977 Jun;27(3):348–351. [PubMed] [Google Scholar]
  25. Suzuki S., Mastroianni L., Jr The fertilizability of in-vitro cultured monkey ovarian follicular oocytes. Fertil Steril. 1968 Jul-Aug;19(4):500–508. doi: 10.1016/s0015-0282(16)36722-x. [DOI] [PubMed] [Google Scholar]
  26. Toyoda Y., Chang M. C. Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer. J Reprod Fertil. 1974 Jan;36(1):9–22. doi: 10.1530/jrf.0.0360009. [DOI] [PubMed] [Google Scholar]
  27. Walton E. A., Armstrong D. T. Oocyte normality after superovulation in immature rats. J Reprod Fertil. 1983 Mar;67(2):309–314. doi: 10.1530/jrf.0.0670309. [DOI] [PubMed] [Google Scholar]
  28. Wehrenberg W. B., Dierschke D. J., Wolf R. C. Uteroovarian pathways and maintenace of early pregnancy in rhesus monkeys. Biol Reprod. 1979 Apr;20(3):601–605. doi: 10.1095/biolreprod20.3.601. [DOI] [PubMed] [Google Scholar]
  29. Weick R. F., Dierschke D. J., Karsch F. J., Butler W. R., Hotchkiss J., Knobil E. Periovulatory time courses of circulating gonadotropic and ovarian hormones in the rhesus monkey. Endocrinology. 1973 Nov;93(5):1140–1147. doi: 10.1210/endo-93-5-1140. [DOI] [PubMed] [Google Scholar]
  30. Whittingham D. G. Fertilization of mouse eggs in vitro. Nature. 1968 Nov 9;220(5167):592–593. doi: 10.1038/220592a0. [DOI] [PubMed] [Google Scholar]

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