Abstract
Purpose/Methods: Clinical outcome after intracytoplasmic sperm injection (ICSI) was evaluated in relation to three techniques of sperm recovery, mini-Percoll, simple concentration, and centrifugation and washing.
Results: Whereas fertilization and embryonic cleavage rates were similar in the three groups, the rates of implantation and clinical pregnancy were statistically significantly higher following sperm recovery by the techniques of mini-Percoll and centrifugation and washing.
Key words: pregnancy rates, intracytoplasmic sperm injection, sperm recovery techniques
Full Text
The Full Text of this article is available as a PDF (356.0 KB).
References
- 1.Mortimer D. Sperm recovery techniques to maximise fertilising capacity. Reprod Fertil Dev. 1994;6:25–31. doi: 10.1071/RD9940025. [DOI] [PubMed] [Google Scholar]
- 2.Yovich JL, Stanger JD. The limitation of in vitro fertilization from males with severe oligospermia and abnormal sperm morphology. J Vitro Fert Embryo Transfer. 1984;1:172–172. doi: 10.1007/BF01139210. [DOI] [PubMed] [Google Scholar]
- 3.Akerlof E, Fredricsson B, Gustafson O, Lunell NO, Nylund L, Rosenborg L, Slotte H, Pousette A. Sperm count and motility influence the results of human fertilization in vitro. Int J Androl. 1991;14(2):79–86. doi: 10.1111/j.1365-2605.1991.tb01068.x. [DOI] [PubMed] [Google Scholar]
- 4.Silber SJ, Steirteghem AC, Lui J, Tournaye H, Devroey P. High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy. Hum Reprod. 1995;10:148–152. doi: 10.1093/humrep/10.1.148. [DOI] [PubMed] [Google Scholar]
- 5.WHO Laboratory Manual for the Examination of Human Semen and Sperm—Cervical Mucus Interaction. 3rd ed. Cambridge: Cambridge University Press; 1992. [Google Scholar]
- 6.Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Merwe JP, Zyl JA, Smith K. Sperm morphologic features as a prognostic factor in vitro fertilisation. Fertil Steril. 1986;46:1118–1123. doi: 10.1016/s0015-0282(16)49891-2. [DOI] [PubMed] [Google Scholar]
- 7.Ord T, Patrizio P, Marello E, Balmaceda J, Asch RH. Mini-Percoll: A new method of semen preparation for IVF in severe male factor infertility. Hum Reprod. 1990;5(8):987–989. doi: 10.1093/oxfordjournals.humrep.a137233. [DOI] [PubMed] [Google Scholar]
- 8.Pickering SJ, Elemin JP, Braude PR, Bolton VN, Gresham GAG. Are human spermatozoa separated on a Percoll density gradient safe for therapeutic use? Fertil Steril. 1989;51:1024–1029. [PubMed] [Google Scholar]
- 9.Egbase PE, Al-Sharhan M, Al-Mutawa M, Al-Othman S, Grudzinskas JG. Mimicking the high levels of activity of a large in-vitro fertilization unit leads to early success at the commencement of an in vitro fertilization and embryo transfer programme. Hum Reprod. 1997;11:2127–2129. doi: 10.1093/oxfordjournals.humrep.a019061. [DOI] [PubMed] [Google Scholar]
- 10.Steirteghem AC, Nagy Z, Joris H, Lui Jiaen, Staessen C, Smitz J, Wisanto A. Devroey: High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod. 1993;8(7):1061–1066. doi: 10.1093/oxfordjournals.humrep.a138192. [DOI] [PubMed] [Google Scholar]
- 11.Payne D, Flaherty SP, Jeffery R, et al. Successful treatment of severe male factor infertility in 100 consecutive cycles using intracytoplasmic sperm injection. Hum Reprod. 1994;9:2051–2057. doi: 10.1093/oxfordjournals.humrep.a138392. [DOI] [PubMed] [Google Scholar]
- 12.Bourne H, Richings N, Liu DY, Clarke GN, Harari O, Baker HW. Sperm preparation for intracytoplasmic injection: Methods and relationships to fertilization results. Reprod Fertil Dev. 1995;7(2):177–183. doi: 10.1071/RD9950177. [DOI] [PubMed] [Google Scholar]
- 13.Zwalmen, Bertin-Segal G, Geerts L, Debauche C, Schoysman R. Sperm morphology and IVF pregnancy rate: Comparison between Percoll gradient centrifugation and swim-up procedures. Hum Reprod. 1991;6(4):581–588. doi: 10.1093/oxfordjournals.humrep.a137383. [DOI] [PubMed] [Google Scholar]