Abstract
Purpose
Our purpose was to assess the morphology and ultrastructural changes in the tubal epithelium following catheterization of the fallopian tube.
Methods
Fallopian tubes were obtained from 20 women who had undergone hysterectomies. Catheterization was performed in 20 tubes using a catheter developed for hysteroscopic tubal embryo transfer. The catheter has a — French diameter, tapering to 2 French (0.66 mm) at the tip portion. The 20 contralateral tubes served as controls and were not catheterized. Ultrastructural changes were examined by scanning electron microscopy and transmission electron microscopy.
Results
Scanning electron microscopy showed no transformation or defects of the tubal epithelium surface in catheterized or control tubes. Transmission electron microscopy showed no significant differences in the percentage of abnormal desmosomes and the percentage of basement membrane in ciliated and nonciliated cells between catheterized and noncatheterized tubes. No transformation or defects were observed in catheterized or noncatheterized tubes.
Conclusions
These findings suggest that catheterization of the tube using a hysteroscopic catheter caused no acute damage to the tubal epithelium.
Key words: catheterization, fallopian tube, scanning electron microscopy, transmission electron microscopy
Full Text
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References
- 1.Asch RH, Balmaceda JP, Ellsworth LR, Wong PC. Gamete intra-fallopian transfer (GIFT): A new treatment for infertility. Int J Fertil. 1985;30:41–45. [PubMed] [Google Scholar]
- 2.Bustillo M, Schulman JD. Transcervical ultrasound-guided intra-fallopian placement of gametes, zygotes, and embryos. J Vitro Fert Embryo Transfer. 1989;6:321–324. doi: 10.1007/BF01138769. [DOI] [PubMed] [Google Scholar]
- 3.Yovich JL, Blackledge DG, Richardson PA, Matson PL, Turner SR, Draper R. Pregnancies following pronuclear stage tubal transfer. Fertil Steril. 1987;48:851–857. doi: 10.1016/s0015-0282(16)59543-0. [DOI] [PubMed] [Google Scholar]
- 4.Gleicher N, Confino E, Corfman R, Coulam C, DeCherney A, Haas G, Katz E, Robinson E, Tur-Kaspa I, Vermesh M. The multicentre transcervical balloon tuboplasty study: Conclusions and comparison to alternative technologies. Hum Reprod. 1993;8:1264–1271. doi: 10.1093/oxfordjournals.humrep.a138238. [DOI] [PubMed] [Google Scholar]
- 5.Miyazaki T, Kobayashi T, Kuji N, Gabionza DT, Sueoka K, Natori M, Nozawa S. Evaluation of the radiologic findings on hysterosalpingography by selective hydrotubation with flexible hysterofiberscope. J Assist Reprod Genet. 1995;12:369–374. doi: 10.1007/BF02215728. [DOI] [PubMed] [Google Scholar]
- 6.Woodruff JD, Pauerstein CJ. Structure, Function, Management. Baltimore: Williams & Wilkins; 1969. The Fallopian Tube; pp. 46–46. [Google Scholar]
- 7.Jansen RPS, Anderson JC. Catheterization of the fallopian tubes from the vagina. Lancet. 1987;2:309–310. doi: 10.1016/S0140-6736(87)90893-2. [DOI] [PubMed] [Google Scholar]
- 8.Risquez F, Bover P, Rolet F, Magnani M, Guichard A, Cedarad L, Zorn JR. Retrograde tubal transfer of human embryos. Hum Reprod. 1990;5:185–188. doi: 10.1093/oxfordjournals.humrep.a137066. [DOI] [PubMed] [Google Scholar]
- 9.Patton PE, Hickok LR, Wolf DP. Successful hysteroscopic cannulation and transfer of cryopreserved embryos. Fertil Steril. 1991;55:640–642. doi: 10.1016/s0015-0282(16)54202-2. [DOI] [PubMed] [Google Scholar]
- 10.Otubu JAM, Winston RML, Wineman M, Ryder T. Morphology of human and experimental hydrosalpinges: A comparative study. Afr J Med Sci. 1987;16:79–88. [PubMed] [Google Scholar]
- 11.Donnez J, Casanas-Roux F, Ferin J, Thomas K. Fimbrial ciliated cells percentage and epithelial height during and after salpingitis. Eur J Obstet Gynecol Reprod Biol. 1984;17:293–299. doi: 10.1016/0028-2243(84)90072-8. [DOI] [PubMed] [Google Scholar]
- 12.Tarn RPL, Mao KR, Mac-Moune Lai. The ultrastructural changes of blocked fallopian tubes. Br J Obstet Gynaecol. 1988;95:802–807. doi: 10.1111/j.1471-0528.1988.tb06555.x. [DOI] [PubMed] [Google Scholar]
- 13.Tanaka K, Tsuda S, Sasai Y. Scanning and transmission electron microscopic studies of lesional epidermis in herpes zoster. J Dermatol. 1994;21:560–570. doi: 10.1111/j.1346-8138.1994.tb01794.x. [DOI] [PubMed] [Google Scholar]
- 14.Crow J, Amso NN, Lewin J, Shaw RW. Morphology and ultrastructure of fallopian tube epithelium at different stages of the menstrual cycle and menopause. Hum Reprod. 1994;9:2224–2233. doi: 10.1093/oxfordjournals.humrep.a138428. [DOI] [PubMed] [Google Scholar]
- 15.Cervera-Paz FJ, Dikkers FG. Ultaestructura y patogenia de las lesiones fonatorias de las cuerdas vocales. Acta Otorrinolaringol (Esp) 1994;45:261–265. [PubMed] [Google Scholar]
- 16.Fritiof SS. The arrangement of mitochondrial membranes and a new structural features of the inner mitochondrial membranes. J Ultrastruct Res. 1977;59:292–319. doi: 10.1016/S0022-5320(77)90041-7. [DOI] [PubMed] [Google Scholar]
- 17.England C, Gartmann M, Zypen E. Diffusion barriers impeding the of solutes to the ciliary body epithelium during immersion fixation of rabbit eyes. Graefe’s Arch Clin Exp Ophthalmol. 1988;226:384–388. doi: 10.1007/BF02172972. [DOI] [PubMed] [Google Scholar]
- 18.Trump BF, Laiho KA, Mergner WJ. The Inflammatory Process. New York: Academic Press; 1965. Some Ultrastructural and Biochemical Consequences of Cell Injury; pp. 35–120. [Google Scholar]
- 19.Bongso A, Ng SC, Sathananthan H, Ng PL, Rauff M, Ratnam S. Improved quality of human embryos when co-cultured with human ampullary cells. Hum Reprod. 1992;4:706–713. doi: 10.1093/oxfordjournals.humrep.a136971. [DOI] [PubMed] [Google Scholar]
- 20.Prichard JF, Thibodeaux JK, Pool SH, Blakewood EG, Menezo Y, Godke RA. In-vitro co-culture of early stage caprine embryos with oviduct and uterine epithelial cells. Hum Reprod. 1992;4:553–557. doi: 10.1093/oxfordjournals.humrep.a137689. [DOI] [PubMed] [Google Scholar]
- 21.Gandolfi F, Moor RM. Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fert. 1987;81:23–28. doi: 10.1530/jrf.0.0810023. [DOI] [PubMed] [Google Scholar]
- 22.Harold GV, Asgerally TF, Kathleen D. The in vitro synthesis and release of proteins by the human oviduct. Endocrinology. 1988;122:4–4. doi: 10.1210/endo-122-4-1639. [DOI] [PubMed] [Google Scholar]