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Journal of Assisted Reproduction and Genetics logoLink to Journal of Assisted Reproduction and Genetics
. 1998 Nov;15(10):594–598. doi: 10.1023/A:1020329326790

Low Blastocyst Formation Rates in Day-2 Fertilized Oocytes

John Gullett 1, George M Grunert 1, Cecilia T Valdes 1, Randall C Dunn 1, Wan-Song A Wun 1
PMCID: PMC3454855  PMID: 9866067

Abstract

Purpose:To examine the blastocyst formation rates of day-2 fertilized oocytes.

Methods:A retrospective study of the outcomes/blastocyst formation of day-2 fertilized oocytes was undertaken.

Results:Fertilization rates of day-1 and -2 oocytes by intracytoplasmic sperm injection were similar. The development frequencies to four cells were similar. However, the blastulation rates were significantly lower from the day-2 fertilized eggs. The fertilization rates from day-2 conventional in vitro fertilization reinsemination were lower than the fertilization rates of day-1 oocytes. The blastulation rates from day-2 fertilized eggs were also lower than the rates from day-1 fertilized eggs in the in vitro fertilization group.

Conclusions:Fertilization is not a good indicator to predict the viability of fertilized oocytes. Day-2 fertilized oocytes had significantly lower blastocyst formation rates than the rates from day-1 fertilized oocytes.

Keywords: blastocyst, development, fertilization, intracytoplasmic sperm injection, in vitro fertilization

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REFERENCES

  • 1.Trounson A, Webb J. Fertilization of human oocytes following reinsemination in vitro. Fertil Steril. 1984;41:816–819. doi: 10.1016/s0015-0282(16)47891-x. [DOI] [PubMed] [Google Scholar]
  • 2.Boldt J, Howe AM, Butler WJ, McDonough PG, Padilla SL. The value of oocyte reinsemination in human in vitro fertilization. Fertil Steril. 1987;48:617–623. doi: 10.1016/s0015-0282(16)59474-6. [DOI] [PubMed] [Google Scholar]
  • 3.Chen HL, Copperman AB, Grunfeld L, Sandler B, Bustillo M, Gordon JW. Failed fertilization in vitro: Second day micromanipulation of oocytes versus reinsemination. Fertil Steril. 1995;63:1337–1340. doi: 10.1016/s0015-0282(16)57622-5. [DOI] [PubMed] [Google Scholar]
  • 4.Lundin K, Sjogren A. Reinsemination of one-day-old oocytes by use of intracytoplasmic injection. Fertil Steril. 1996;66:118–121. [PubMed] [Google Scholar]
  • 5.Wun WSA, Wun CCC, Valdes CT, Dunn RC, Grunert GM. Optimal insemination concentration for human in vitro fertilization. Chinese J Physiol. 1997;40:227–236. [PubMed] [Google Scholar]
  • 6.Van Steirteghem AC, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, Wisanto A, Devroey P. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod. 1993;8:1061–1066. doi: 10.1093/oxfordjournals.humrep.a138192. [DOI] [PubMed] [Google Scholar]
  • 7.Almeida PA, Bolton VN. Immaturity and chromosomal abnormalities in occytes that fail to develop pronuclei following insemination in vitro. Hum Reprod. 1993;8:229–232. doi: 10.1093/oxfordjournals.humrep.a138028. [DOI] [PubMed] [Google Scholar]
  • 8.Check JH, Long R, Allen J, Hoover L. Poor fertilization may be related to oocyte or zona pellucida recognition defects specific to certain hyperstimulation regimens and limited to some males but not others: A case report. Fertil Steril. 1995;63:1341–1343. doi: 10.1016/s0015-0282(16)57623-7. [DOI] [PubMed] [Google Scholar]
  • 9.Wun W. A., Wun C-C, Grunert GM. Minimum essential medium alpha enhances assisted reproductive technology results. I. mouse embryo study. J Assist Reprod Genet. 1994;11:303–307. doi: 10.1007/BF02215717. [DOI] [PubMed] [Google Scholar]
  • 10.Lopata A, Hay DL. The surplus human embryo: Its potential for growth, blastulation, hatching, and human chorionic gonadotropin production in culture. Fertil Steril. 1989;51:984–991. doi: 10.1016/s0015-0282(16)60731-8. [DOI] [PubMed] [Google Scholar]
  • 11.Muggleton-Harris AL, Glazier AM, Wall M. A retrospective analysis of the in-vitro development of “spare” human in-vitro fertilization preimplantation embryos using “in-house” prepared medium and “Medi-Cult” commercial medium. Hum Reprod. 1995;10:2976–2984. doi: 10.1093/oxfordjournals.humrep.a135833. [DOI] [PubMed] [Google Scholar]
  • 12.Gardner DK, Vella P, Lane M, Wagely L, Schlenker, Schoolcraft WB: Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfer. American Society for Reproductive Medicine, 53rd Annual Meeting. Program Supplement, Cincinnati, OH, Oct 18–22, 1997 (abstr) [DOI] [PubMed]
  • 13.Wun WSA, Wun CC, Valdes CT, Dunn RC, Grunert GM. Blastocyst formation is a good indicator for attainment of assisted reproductive reproduction. Chinese J Physiol. 1997;40:237–242. [PubMed] [Google Scholar]
  • 14.Eppig JJ, Schultz RM, O'Brien M, Chesnel F. Relationship between the development programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol. 1994;164:1–9. doi: 10.1006/dbio.1994.1175. [DOI] [PubMed] [Google Scholar]

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