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. 1984 Jun;81(11):3273–3277. doi: 10.1073/pnas.81.11.3273

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

S Cabilly, A D Riggs, H Pande, J E Shively, W E Holmes, M Rey, L J Perry, R Wetzel, H L Heyneker
PMCID: PMC345489  PMID: 6374653

Abstract

Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.

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Selected References

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