Corrigendum

Corrigendum

In the article by J. A. Mayfield, M. W. Davies, D. Dimster-Denk, N. Pleskae, S. McCarthy, E. A. Boydston, L. Fink, X. X. Lin, A. S. Narain, M. Meighan, and J. Rine (GENETICS 190: 1309–1323) entitled “Surrogate Genetics and Metabolic Profiling for Characterization of Human Disease Alleles,” the Clinical Response to B6 column of Table 2 contains a systematic naming error. We offer a corrected version of Table 2 with the proper category names replacing those changed in error by the authors during editing. The authors thank S. Harvey Mudd for detecting the inconsistent entries and apologize for any confusion this error has caused.

Table 2 .

Summary of clinical and yeast phenotypes

Mutation	Clinical response to B6	Enzyme activity	Yeast phenotype and remediation
H65R	Nonresponsive	Lowa	Nonfunctionalb
A69P	NA		Similar to major allele
P70L	NA		Intermediate growth, B6 remedial, hem1 rescue
P78R	Responsive	Meda,c	Similar to major allele
G85R	Partial response	Lowd	High growth, B6 and heme remedial
T87N	ND		Low growthb
P88S	ND		Low growthb
L101P	Variable	Lowe	Nonfunctional
K102N	Responsive	Meda,c	Similar to major alleleb
K102Q	ND		Similar to major allele
C109R	Variable	Lowe	Nonfunctionalb
A114V	Variable	Medf/higha	Low growth
G116R	Responsive		Nonfunctional
R121C	ND		Nonfunctional
R121L	ND		Nonfunctional
R121H	Nonresponsive		Nonfunctional
M126V	Nonresponsive		Nonfunctionalb
E128D	Nonresponsive		Similar to major allele
E131D	Nonresponsive	Lowg	Low growthb
G139R	Responsive		Intermediate growthb
I143M	Nonresponsive	Lowh	Nonfunctional
E144K	Variable	Lowa,i	Nonfunctionalb
P145L	Nonresponsive	Lowj	Nonfunctionalb
G148R	Nonresponsive	Lowa	Nonfunctional
G151R	ND		Nonfunctionalb
I152M	Variable	Lowk	hem1 rescue
G153R	NA		Nonfunctional
L154Q	NA	Lowl	Nonfunctional
A155T	Variable		Low growth, B6 and heme remedial
A155V	NA	Lowl	hem1 rescue
A158V	NA		Low growth
C165Y	Variable	Lowa,k	Nonfunctional
V168M	ND		Nonfunctionalb
E176K	Nonresponsive	Lowa	hem1 rescue
V180A	Responsive	Meda,k	Intermediate growth, B6 remedial
T191M	Nonresponsive	Lowa,k	Nonfunctional
D198V	Nonresponsive		Low growthb
R224H	ND		Low growth
A226T	Responsive	Medm	Low growthb
N228S	Nonresponsive		Nonfunctional
N228K	Nonresponsive	Lowa,d	Nonfunctional
D234N	Nonresponsive		Intermediate growth, heme remedial
E239K	Responsive		Nonfunctional
T257M	Nonresponsive		Nonfunctional
G259S	ND		Nonfunctional
T262M	Nonresponsive		Low growth, B6 remedial
R266G	Nonresponsive		Nonfunctional
R266K	Responsive	Meda	High growth, B6 and heme remedial
C275Y	Nonresponsive	Lowm	Nonfunctional
I278T	Variable	Lowa,n	Low growth
A288P	NA		Nonfunctional
A288T	NA	Lowl	Nonfunctional
P290L	Responsive		hem1 rescue
E302K	Variable	Lowf/higha	Nonfunctional
G307S	Variable	Lowa,o	Nonfunctionalb
V320A	Variable		Intermediate growth, B6 and heme remedial
A331E	ND	Lowi	Nonfunctional
A331V	ND		Low growth
R336H	Responsive	Lowm	hem1 rescue; not tested in liquid media
G347S	Responsive	Lowe	Nonfunctional
S349N	Nonresponsive	Lowm	Nonfunctional
S352N	Nonresponsive		Low growth
T353M	Variable	Lowi	Low growth, B6 and heme remedial
V354M	Nonresponsive		High growth, B6 remedial
A355P	ND		Nonfunctional
A361T	Nonresponsive		Nonfunctional
R369P	NA		Nonfunctional
R369H	ND		Similar to major allele
R369C	Responsive	Lowk/meda	Similar to major allele
C370Y	Responsive		Nonfunctional
V371M	Partial response	Lowk	Similar to major allele
D376N	ND		Nonfunctional
R379W	ND		hem1 rescue
K384E	Responsive		Nonfunctionalb
K384N	Nonresponsive		hem1 rescue
M391I	Nonresponsive		Nonfunctional
P422L	Nonresponsive	Higha,d	Similar to major allele
T434N	Responsive		High growth, B6 remedial
I435T	ND	Higha,d	Similar to major allele
R439Q	Variable	Meda	Similar to major allele
D444N	Variable	Higha/medm	Similar to major allele
L456P	Nonresponsive	Lowm	Intermediate growth
S466L	ND	Higha,d	Similar to major allele
Q526K	Nonresponsive		Intermediate growth, hem1 rescueb

Alleles with yeast growth phenotypes inconsistent with clinical (underlined) or in vitro activity (italics) are indicated. Alleles identified in the clinic but without information about B6 response (ND, no data) and alleles identified from available sequence only (NA, not applicable) are indicated. Enzyme activity summarizes several different assays including expression in Escherichia coli or in human fibroblast cell culture. Low indicates activity at or below the level of detection, med indicates an intermediate activity, and high indicates activity indistinguishable from the major allele.

^aKozich et al. (2010).

^bThe alleles we analyzed by immunoblot. The original publication and a recent reanalysis were cited.

^cde Franchis et al. (1994).

^dMaclean et al. (2002).

^eGaustadnes et al. (2002).

^fde Franchis et al. (1999).

^gMarble et al. (1994).

^hOrendae et al. (2004).

ⁱDawson et al. (1997).

^jKozich et al. (1993).

^kKraus et al. (1999).

^lLee et al. (2005).

^mUrreizti et al. (2006).

ⁿKozich and Kraus (1992).

^oHu et al. (1993).