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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Alcohol. 2012 Jul 12;46(7):635–642. doi: 10.1016/j.alcohol.2012.03.005

Fig. 2.

Fig. 2

Ethanol induces HDACs 1 and 3 genes. After reaching 60% confluency, SK-N-MC were pre-treated with TSA (50 nM) for 2 hours, then treated with ethanol (0.1%) for 48 hours. After incubation, cells were harvested, RNA was extracted and reverse transcribed followed by qRTPCR for HDACs 1 and 3 and housekeeping GAPDH and 18S rRNA gene expression. Ethanol significantly enhanced HDACs 1 and 3, while TSA + ethanol treatments significantly inhibited HDACs 1 and 3 genes. Data are expressed as mean ± SE of TAI values of four independent experiments. Comparisons between groups were performed using one-way ANOVA and Dunnett's Multiple Comparison Test. Comparison between groups revealed significant differences, “Control vs. ethanol ” (*p<0.05) and “ethanol vs. TSA + ethanol” (#p<0.05).