Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Jun;81(11):3399–3403. doi: 10.1073/pnas.81.11.3399

Identification of a 34,000-dalton mitogenic protein associated with plasma membranes from human A431 epidermoid carcinoma cells.

S Bishayee, D Matesic, M Das
PMCID: PMC345515  PMID: 6587356

Abstract

We present evidence for a discrete 34,000-Da polypeptide with mitogenic activity, associated with plasma membranes from human A431 carcinoma cells. Plasma membranes from A431 cells are highly mitogenic for quiescent fibroblasts. A significant fraction of the membrane-associated activity can be released by treatment with a high concentration of salt and is relatively acid stable. Incubation of 125I-labeled salt-extracted proteins with target fibroblasts results in preferential binding of a 34,000-Da protein--i.e., greater than 90% of the cell-bound radioactivity is associated with the 34,000-Da polypeptide. Studies correlating the mitogenic activity with the cell-binding 34,000-Da protein indicate that this protein is the acid-stable, peripherally attached, mitogenically active component of A431 membranes. Available data suggest that this protein may be mitogenically active in the nanomolar concentration range. Some properties of this protein are described.

Full text

PDF
3399

Images in this article

3400Image
on p.3400
3401Image
on p.3401
3401Image
on p.3401
3402Image
on p.3402

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoniades H. N., Scher C. D., Stiles C. D. Purification of human platelet-derived growth factor. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1809–1813. doi: 10.1073/pnas.76.4.1809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishayee S., Das M. A hepatic membrane-associated factor stimulates nuclear DNA synthesis in cultured fibroblastic cells. Biochim Biophys Acta. 1982 Feb 26;696(2):134–138. doi: 10.1016/0167-4781(82)90020-3. [DOI] [PubMed] [Google Scholar]
  3. Bishayee S., Feinman J., Pittenger M., Michael H., Das M. Cell surface insertion of exogenous epidermal growth factor receptors into receptor- mutant cells: demonstration of insertion in the absence of added fusogenic agents. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1893–1897. doi: 10.1073/pnas.79.6.1893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  5. Czech M. P. Structural and functional homologies in the receptors for insulin and the insulin-like growth factors. Cell. 1982 Nov;31(1):8–10. doi: 10.1016/0092-8674(82)90399-3. [DOI] [PubMed] [Google Scholar]
  6. Das M., Fox C. F. Molecular mechanism of mitogen action: processing of receptor induced by epidermal growth factor. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2644–2648. doi: 10.1073/pnas.75.6.2644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Das M. Initiation of nuclear DNA replication: evidence for formation of committed prereplicative cellular state. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5677–5681. doi: 10.1073/pnas.78.9.5677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Das M. Mitogenic hormone-induced intracellular message: assay and partial characterization of an activator of DNA replication induced by epidermal growth factor. Proc Natl Acad Sci U S A. 1980 Jan;77(1):112–116. doi: 10.1073/pnas.77.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Das M., Miyakawa T., Fox C. F., Pruss R. M., Aharonov A., Herschman H. R. Specific radiolabeling of a cell surface receptor for epidermal growth factor. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2790–2794. doi: 10.1073/pnas.74.7.2790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frolik C. A., Dart L. L., Meyers C. A., Smith D. M., Sporn M. B. Purification and initial characterization of a type beta transforming growth factor from human placenta. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3676–3680. doi: 10.1073/pnas.80.12.3676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lieberman M. A. Mitogenic proteins of the 3T3 plasma membrane. J Cell Physiol. 1983 Jan;114(1):73–76. doi: 10.1002/jcp.1041140112. [DOI] [PubMed] [Google Scholar]
  13. Natraj C. V., Datta P. Control of DNA synthesis in growing BALB/c 3T3 mouse cells by a fibroblast growth regulatory factor. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6115–6119. doi: 10.1073/pnas.75.12.6115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pruss R. M., Herschman H. R. Variants of 3T3 cells lacking mitogenic response to epidermal growth factor. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3918–3921. doi: 10.1073/pnas.74.9.3918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salzer J. L., Bunge R. P., Glaser L. Studies of Schwann cell proliferation. III. Evidence for the surface localization of the neurite mitogen. J Cell Biol. 1980 Mar;84(3):767–778. doi: 10.1083/jcb.84.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thom D., Powell A. J., Lloyd C. W., Rees D. A. Rapid isolation of plasma membranes in high yield from cultured fibroblasts. Biochem J. 1977 Nov 15;168(2):187–194. doi: 10.1042/bj1680187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whittenberger B., Glaser L. Inhibition of DNA synthesis in cultures of 3T3 cells by isolated surface membranes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2251–2255. doi: 10.1073/pnas.74.6.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES