Abstract
Purpose: Maturation promoting factor (MPF) acts at theresumption of meiosis and nonspecifically throughout theanimal species. There exists a considerable body of literatureon MPF, but little work has been done to study the inductionof maturation of mammalian oocytes by microinjection ofextracted MPF.
Methods: Immature (GV-stage) mouse oocytes weremicroinjected MPF extracted from matured Xenopus eggs in thepresense of dbcAMP.
Results: The rate of germinal vesicle breakdown (GVBD)induced at 24 hr after MPF injection was significantly higher(90.5%) than that of the control (2.2%), which was injectedwith HTF medium containing dbcAMP (P < 0.0001). Therate of extrusion of the first polar body at 24 hr after MPFinjection was significantly higher (84.1%) than that of thesame control (1.1%) (P < 0.0001).
Conclusions: From these results, it is concluded that thematuration of mammalian oocytes can be induced by themicroinjection of MPF extracted from other species.
Keywords: maturation promoting factor, immature oocyte, in vitro maturation, microinjection
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