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. 2001 Dec;18(12):623–628. doi: 10.1023/A:1013171505702

A Prospective Study on Oocyte Survival Rate After ICSI: Influence of Injection Technique and Morphological Features

Thomas Ebner 1, Cemil Yaman 1, Marianne Moser 1, Michael Sommergruber 1, Klaus Jesacher 1, Gernot Tews 1
PMCID: PMC3455248  PMID: 11808841

Abstract

Purpose: To determine the influence of technical pitfalls and oocyte morphology on survival rate and cleavage behavior after ICSI.

Methods: A total of 2210 injection procedures was examined for morphological and technical deviations. Survival rate and cleavage behavior were evaluated.

Results: In 77.8% of all cases ICSI was unsuspicous. Out of 491 deviations from optimal injection deep penetration of the oocyte and abundant presence of cumulus cells showed significant correlation with degeneration rate (p < 0.001). Morphological anomalies associated with the periphery of the oocyte were rather related to degeneration than cytoplasmic anomalies (p < 0.001). Early embryonic development was not impaired by technical or morphological parameters.

Conclusions: To conclude, these prospective data may be of prognostic value in regard of the number of embryos available for transfer and may help to improve treatment outcome.

Keywords: degeneration of oocytes, fertilization rate, ICSI technique, morphology of oocyte

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