Abstract
Purpose: To report a delivery after transfer of blastocysts derived from eggs collected following in vivo HCG priming in a patient with regular menstrual cycles undergoing in vitro maturation (IVM) program.
Methods: A woman had regular menstrual cycle and had experience of ovarian hyperstimulation syndrome (OHSS) during a previous conventional IVF-ET cycle. The patient was primed with 10,000 IU HCG 36 h before egg retrieval. After oocyte collection, the maturity of oocytes was evaluated and immature oocytes were cultured in IVM medium. The matured oocytes were fertilized with husband sperm, and normal fertilized eggs were cultured to blastocysts stage until embryo transfer in uteri.
Results: Three MII-stage and 13 GV-stage oocytes were collected from the patient. Three mature oocytes were fertilized by conventional IVF. All three fertilized oocytes were developed to blastocysts. Immature oocytes were matured in vitro and insemination was carried out by ICSI. Out of eight fertilized zygotes, two developed to blastocyst stage. Transfer of three expanded blastocysts on Day 6 resulted in pregnancy in the patient and one healthy baby was born.
Conclusions: This report provides an approach to treat infertile women with regular menstrual cycle and high risk of OHSS.
Keywords: Blastocyst, IVM, pregnancy
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REFERENCES
- 1.Chain RC, Buckett WM, Tulandi T, Tan SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrireview from unstimulated women with polycystic ovarian syndrome. Hum Reprod. 2000;15:165–170. doi: 10.1093/humrep/15.1.165. [DOI] [PubMed] [Google Scholar]
- 2.Son WY, Yoon SH, Lee SW, Ko Y, Yoon HG, Lim JH. Blastocyst development and pregnancies after in vitro fertilization of mature oocytes retrieved from unstimulated patients with PCOS after in vivo HCG priming. Hum Reprod. 2002;17:134–136. doi: 10.1093/humrep/17.1.134. [DOI] [PubMed] [Google Scholar]
- 3.Yoon HG, Yoon SH, Son WY, Lee SW, Park SP, Im KS, Lim JH. Pregnancies resulting from in vitro matured oocytes collected from women with regular menstrual cycle. J Assist Reprod Genet. 2001;18:249–253. doi: 10.1023/A:1016632621452. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkai AM, Tiglias J, Wood C, Trounso A. Blastocyst development and birth after in vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10:3243–3247. doi: 10.1093/oxfordjournals.humrep.a135896. [DOI] [PubMed] [Google Scholar]