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. 2000 Jul;17(6):323–328. doi: 10.1023/A:1009453011321

Influence of Zona Pellucida Thickness of Human Embryos on Clinical Pregnancy Outcome Following In Vitro Fertilization Treatment

Anette Gabrielsen 1, Piyush R Bhatnager 2, Karsten Petersen 1, Svend Lindenberg 3
PMCID: PMC3455392  PMID: 11042829

Abstract

Purpose: To evaluate the correlation between the degree ofzona pellucida thickness variation (ZPTV) of transferredembryos with identical morphologies and subsequent clinicalpregnancy rates during 141 intracytoplasmic sperminjection (ICSI) treatment cycles.

Methods: A total of 141 women participating in the studygroup comprising mostly male factor infertilities and somerepeat in vitro fertilization (IVF) failures were transferred,2 days after ovum pickup, with two to three embryos withidentical grades created by fertilization with ICSI. Allselected embryos were subjected to zona pellucida thicknessmeasurements immediately prior to transfer using a computerizedembryo measurement program from videocinematographyrecordings.

Results: A total of 326 identical-grade transferredpre-embryos resulted in 70 clinical pregnancies with live-bornimplantation rate of 27.6%. A highly significant correlationwas observed between ZPTV of transferred embryos andthe IVF outcome with 77.1% and 83.64% of the clinicalpregnancies resulting from transferred embryos with ZPTVvalues greater than 20 and 25, respectively. The mean ZPTVvalues for 70 conceptual cycles and 62 nonconceptual cycleswere 28 ± 6.43 and 17.85 ± 8.11, respectively. No significantcorrelation between ZP thickness and number of blastomeresin the transferred embryos was evident, though embryoswith better scores had significantly thinner zonae and higherZPTV values. Though average zona thickness of embryosdeclined with age, the mean ZPTV value for women lessthan 30 years old was significantly higher (25.84 ± 8.57)as compared with those from women older than 35 years(20.72 ± 8.45).

Conclusions: The degree of ZPTV of the transferred embryosexhibits a strong correlation with clinical pregnancy outcome following IVF treatment. This potentially reliable indicatorof IVF success rate could be used as a criteria forembryo selection during clinical transfers.

Keywords: Clinical IVF, embryo, embryo transfer, pregnancy, zona pellucida

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REFERENCES

  • 1.Spaczynski RZ, Duleba AJ. In vitro fertilization: Predictors of success. Assist Reprod Rev. 1996;6(4):150–161. [Google Scholar]
  • 2.Daniel SA. Factors influencing outcomes after in vitro fertilization. Assist Reprod Rev. 1995;5(3):204–213. [Google Scholar]
  • 3.Saith RR, Sargent IL. Embryo selection for transfer in human in vitro fertilization. Assist Reprod Rev. 1995;5(3):145–154. [Google Scholar]
  • 4.Roseboom TJ, Vermeiden JPW. Evaluation of embryo scoring systems and their value in predicting in vitro fertilization outcome. Assist Reprod Rev. 1995;5(1):53–59. [Google Scholar]
  • 5.Cohen J, Inge KL, Suzman K, Wiker SR, Wright R. Videocinematography of fresh and cryopreserved embryos: A retrospective analysis of embryonic morphology and implantation. Fertil Steril. 1989;51:820–827. doi: 10.1016/s0015-0282(16)60673-8. [DOI] [PubMed] [Google Scholar]
  • 6.Bertrand E, Van den Beergh M, Englert Y. Does zona pellucida thickness influence the fertilization rate? Hum Reprod. 1995;10(5):1189–1193. doi: 10.1093/oxfordjournals.humrep.a136116. [DOI] [PubMed] [Google Scholar]
  • 7.Loret De Mola JR, Garside WT, Bucci J, Tureck RW, Heyner S. Analysis of the human zona pellucida during culture: correlation with diagnosis and preovulatory hormonal environment. J. Assist Reprod Genet. 1997;14(6):332–336. doi: 10.1007/BF02765837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Garside WT, Loret De Mola JR, Bucci JA, Tureck RW, Heyner S. Sequential analysis of zona pellucida thickness during in vitro culture of human zygotes: Correlation with embryo quality, age and implantation. Mol Reprod Dev. 1997;47:99–104. doi: 10.1002/(SICI)1098-2795(199705)47:1<99::AID-MRD13>3.0.CO;2-V. [DOI] [PubMed] [Google Scholar]
  • 9.Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod. 1992;7:685–691. doi: 10.1093/oxfordjournals.humrep.a137720. [DOI] [PubMed] [Google Scholar]
  • 10.Bertrand E, Van den Bergh M, Englert Y. Clinical parameters influencing human zona pellucida thickness. Fertil Steril. 1996;66:408–411. [PubMed] [Google Scholar]
  • 11.Letterie GS. Assisted hatching: rationale, technique and clinical outcomes. Assist Reprod Rev. 1997;8(3):116–125. [Google Scholar]
  • 12.Cohen J. Assisted hatching of human embryos. J In Vitro Fertil Embryo Transfer. 1991;8:179–191. doi: 10.1007/BF01130802. [DOI] [PubMed] [Google Scholar]
  • 13.DeFelici M, Siracusa G. Spontaneous hardening of the zona pellucida of mouse oocytes during in vitro culture. Gamete Res. 1982;6:107–113. [Google Scholar]
  • 14.Palmstierna M, Murkes D, Csemiczky G, Andersson O, Wramsby H. Zona pellucida thickness variation and occurence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment. J Assist Reprod Genet. 1998;15(2):70–75. doi: 10.1007/BF02766828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Gabrielsen A, Petersen K, Mikkelsen AL, Lindenberg S. Intracytoplasmic sperm injection does not overcome an oocyte defect in previous fertilization failure with conventional in vitro fertilization and normal spermatozoa. Hum Reprod. 1996;11(9):1963–1965. doi: 10.1093/oxfordjournals.humrep.a019525. [DOI] [PubMed] [Google Scholar]
  • 16.Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, Van Zyl JA, Smith K. Sperm morphologic features as a prognostic factor in in vitro fertilizaton. Fertil Steril. 1986;46:1118–1123. doi: 10.1016/s0015-0282(16)49891-2. [DOI] [PubMed] [Google Scholar]
  • 17.Mortimer D. Sperm preparation techniques and iatrogenic failures in in-vitro fertilization. Hum Reprod. 1991;6:173–176. doi: 10.1093/oxfordjournals.humrep.a137300. [DOI] [PubMed] [Google Scholar]
  • 18.Veeck LL. Atlas of the Human Oocyte and Early Conceptus, Vol. 2. Baltimore: Williams and Wilkins; 1991. [Google Scholar]
  • 19.Van den Abbeel E, Van der Elst J, Van Waesberghe I, et al. Hyperstimulation: the need for cryopreservation of embryos. Hum Reprod. 1988;3:53–57. doi: 10.1093/humrep/3.suppl_2.53. [DOI] [PubMed] [Google Scholar]
  • 20.Staessen C, Van den Abbeel E, Carte M, Khan I, Devroey P, Van Steirteghem AC. Comparison between human serum and Albuminar-29TM supplement for in vitro fertilization. Hum Reprod. 1990;5:336–341. doi: 10.1093/oxfordjournals.humrep.a137100. [DOI] [PubMed] [Google Scholar]
  • 21.Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: How to select the best embryos for transfer after in vitro fertilization. Hum Reprod. 1997;12(7):1545–1549. doi: 10.1093/humrep/12.7.1545. [DOI] [PubMed] [Google Scholar]
  • 22.Schwartz D. Méthodes statistiques a l' usage des médecins et des biologistes. In: Schwartz D, editor. Med.-Sciences. 4th ed. Paris: Flammarion; 1993. p. 314. [Google Scholar]
  • 23.Hosmer DW, Lemeshow S. Applied Logistic Regression. New York: John Wiley and Sons; 1989. [Google Scholar]
  • 24.Cohen J, Feldberg D. Effects of the site and number of zona pellucida openings on hatching and trophoblast outgrowth in the mouse embryo. Mol Reprod Dev. 1991;30:70–78. doi: 10.1002/mrd.1080300110. [DOI] [PubMed] [Google Scholar]
  • 25.Chan PJ. Developmental potential of human oocytes according to zona pellucida thickness. J In Vitro Fert Embryo Transfer. 1987;4:237. doi: 10.1007/BF01533763. [DOI] [PubMed] [Google Scholar]
  • 26.Cohen J, Wiemer KE, Wright G. Prognostic value of morphologic characteristics of cryopreserved embryos: A study using videocinematography. Fertil Steril. 1988;49:827. doi: 10.1016/s0015-0282(16)59892-6. [DOI] [PubMed] [Google Scholar]
  • 27.Wright G, Wiker S, Elsner C, Kort H, Massey J, Mitchell D, Toledo A, Cohen J. Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation. Hum Reprod. 1990;5:109–115. doi: 10.1093/oxfordjournals.humrep.a137029. [DOI] [PubMed] [Google Scholar]
  • 28.Cohen J, Alikani M, Reing AM, Ferrara TA, Trowbridge J, Tucker M. Selective hatching of human embryos. Ann Acad Med. 1992;21:565–570. [PubMed] [Google Scholar]
  • 29.Cohen J. Assisted hatching of human embryos. J Assist Reprod Genet. 1993;8:179–190. [Google Scholar]
  • 30.Liu HC, Cohen J, Alikani M, Noyes N, Rosenwaks Z. Assisted hatching facilitates earlier implantation. Fertil Steril. 1993;60:871–875. [PubMed] [Google Scholar]

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