Abstract
Purpose: To assess the influence of different concentrationsof recombinant human leukemia inhibitory factor (LIF) onthe in vitro development of mouse embryos.
Methods: The 2- to 4-cell embryos of CB6F1 mice werecultured in the human tubal fluid (HTF) media containingdifferent concentrations of LIF. Mouse embryos were dividedinto seven groups: (1) HTF; (2) 1500 IU/ml LIF; (3) 1000IU/ml LIF; (4) 750 IU/ml LIF; (5) 500 IU/ml LIF; (6) 250IU/ml LIF; (7) 125 IU/ml LIF. The embryonic numbers ofdifferent stages including 5–8 cell, 9–16 cell, morula, blastocyst,and hatching blastocyst were recorded.
Results: The percentage of early embryo stage (2-cellembryos to 6- to 16-cell stages) in all groups were nonsignificantlydifferent. There were higher formation rates of preimplantationembryos (morula to hatching blastocyst) ingroups 2, 3, 4, and 5 than in groups 1, 6 and 7.
Conclusions: LIF has positive effects on preimplantationembryo development and has nonsignificant influence onthe early embryo development. The lowest concentration ofLIF which could provide the optimal embryo developmentis 500 IU/ml.
Keywords: Leukemia inhibitory factor, recombinant LIF, coculture
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