Abstract
Adrenal enkephalin-containing peptides are known to increase 10- to 15-fold in the rat after surgical denervation of the gland. In this report we show that the increase is preceded by a lag of several hours, which is indicative of stimulation of protein synthesis at the transcriptional level. The major species of newly appearing enkephalin-containing peptide appears to be the intact precursor, proenkephalin. Processing of proenkephalin to smaller enkephalin-containing peptides in the denervated glands is slow and limited. The only product that accumulates in the process is a peptide of 3-4 kilodaltons that is derived from the carboxyl terminus of proenkephalin. An interesting observation was the dissociation between the effects of denervation on enkephalin-containing peptides and catecholamines. This is surprising because both are localized in the chromaffin granules of the gland.
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Selected References
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- Böhlen P., Stein S., Dairman W., Udenfriend S. Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973 Mar;155(1):213–220. doi: 10.1016/s0003-9861(73)80023-2. [DOI] [PubMed] [Google Scholar]
- Fleminger G., Ezra E., Kilpatrick D. L., Udenfriend S. Processing of enkephalin-containing peptides in isolated bovine adrenal chromaffin granules. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6418–6421. doi: 10.1073/pnas.80.20.6418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilpatrick D. L., Jones B. N., Lewis R. V., Stern A. S., Kojima K., Shively J. E., Udenfriend S. An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences. Proc Natl Acad Sci U S A. 1982 May;79(9):3057–3061. doi: 10.1073/pnas.79.9.3057. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kojima K., Kilpatrick D. L., Stern A. S., Jones B. N., Udenfriend S. Proenkephalin: a general pathway for enkephalin biosynthesis in animal tissues. Arch Biochem Biophys. 1982 May;215(2):638–643. doi: 10.1016/0003-9861(82)90125-4. [DOI] [PubMed] [Google Scholar]
- Lahm H. W., Gerber L. D., Brink L., Kilpatrick D. L., Udenfriend S. Specific polyclonal antibodies to the carboxyl terminus of [Met]enkephalin-Arg6-Gly7-Leu8. Arch Biochem Biophys. 1983 Sep;225(2):422–429. doi: 10.1016/0003-9861(83)90049-8. [DOI] [PubMed] [Google Scholar]
- Lewis R. V., Stern A. S., Kilpatrick D. L., Gerber L. D., Rossier J., Stein S., Udenfriend S. Marked increases in large enkephalin-containing polypeptides in the rat adrenal gland following denervation. J Neurosci. 1981 Jan;1(1):80–82. doi: 10.1523/JNEUROSCI.01-01-00080.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis R. V., Stern A. S., Kimura S., Rossier J., Stein S., Udenfriend S. An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin. Science. 1980 Jun 27;208(4451):1459–1461. doi: 10.1126/science.7384787. [DOI] [PubMed] [Google Scholar]
- Lewis R. V., Stern A. S., Rossier J., Stein S., Udenfriend S. Putative enkephalin precursors in bovine adrenal medulla. Biochem Biophys Res Commun. 1979 Aug 13;89(3):822–829. doi: 10.1016/0006-291x(79)91852-7. [DOI] [PubMed] [Google Scholar]
- Schultzberg M., Lundberg J. M., Hökfelt T., Terenius L., Brandt J., Elde R. P., Goldstein M. Enkephalin-like immunoreactivity in gland cells and nerve terminals of the adrenal medulla. Neuroscience. 1978;3(12):1169–1186. doi: 10.1016/0306-4522(78)90137-9. [DOI] [PubMed] [Google Scholar]
- Stern A. S., Jones B. N., Shively J. E., Stein S., Undenfriend S. Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1962–1966. doi: 10.1073/pnas.78.3.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Udenfriend S., Kilpatrick D. L. Biochemistry of the enkephalins and enkephalin-containing peptides. Arch Biochem Biophys. 1983 Mar;221(2):309–323. doi: 10.1016/0003-9861(83)90149-2. [DOI] [PubMed] [Google Scholar]
- VON EULER U. S., FLODING I. A fluorimetric micromethod for differential estimation of adrenaline and noradrenaline. Acta Physiol Scand Suppl. 1955;33(118):45–56. [PubMed] [Google Scholar]