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. 2000 Mar;17(3):131–134. doi: 10.1023/A:1009426303742

Clinical Assisted Reproduction: Prolonged Culture of Human Cryopreserved Embryos with Recombinant Human Leukemia Inhibitory Factor

Yao-Yuan Hsieh 1, Horng-Der Tsai 1,2, Chi-Chen Chang 1, Li-Wei Hsu 1, Su-Chen Chang 1,3, Hui-Yu Lo 1
PMCID: PMC3455658  PMID: 10911571

Abstract

Purpose: To evaluate the efficiency of recombinant humanleukemia inhibitory factor (LIF) in the prolonged culture ofhuman cryopreserved-thawing embryos.

Methods: After thawing, all embryos were divided into fourgroups: (1) Human tubal fluid (HTF), (2) HTF + LIF, (3)M3TH medium, and (4) M3TH medium plus LIF. Followingprolonged culture, embryo development in each groupwas compared.

Results: In embryo development from about the 2– to 4–cellto 9– to 16–cell stage, there were nonsignificant differencesbetween each group. There was lower morula formation ratein group 1 (6.9%) than those in other groups (23.2%, 19.7%,23.1%). The lower blastocyst formation in group 1 and 3(0%, 0%) than those in group 2 and 4 (11.0%, 12.8%)were noted.

Conclusions: LIF is beneficial for preimplantation embryos.LIF does not influence the early embryo development.LIF-supplemented HTF provided a similar culture environmentfor thawing embryos as LIF-supplemented M3TH medium.

Keywords: Blastocyst, leukemia inhibitory factor, prolonged culture

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Footnotes

Supported by China Medical College Hospital, Taiwan

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