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. 2000 Mar;17(3):162–167. doi: 10.1023/A:1009470206468

Hyaluronic Acid Inhibits Apoptosis in Granulosa Cells via CD44

Tomoko Kaneko 1, Hidekazu Saito 1, Mayumi Toya 1, Takakazu Satio 1, Kenji Nakahara 1, Masahiko Hiroi 1
PMCID: PMC3455659  PMID: 10911577

Abstract

Purpose: This study was designed to examine whether hyaluronicacid (HA) inhibits apoptosis in cumulus and muralgranulosa cells and to examine whether this effect of HAwas mediated through CD44.

Methods: Mural and cumulus granulosa cells were obtainedfrom in vitro fertilization patients. The cells were culturedwith various concentrations of HA or HA plus variousconcentrations of anti-CD44 antibody without serum supplement.After 24 hr of culture, the cells were fixed and stainedwith Hoechst 33258. One thousand granulosa cells of eachconditions were observed by fluorescence microscopy.

Results: HA inhibited apoptosis in both kinds of granulosacells, and anti-CD44 antibody prevented this effect of HA.Conclusions: The incidence of apoptotic granulosa cellswith fragmented condensed nuclei was reduced by HA viaCD44.

Keywords: Apoptosis, CD44, human, hyaluronic acid, granulosa cells

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