Abstract
Purpose: The present study was to investigate the impact of type and state of co-culture cells on developmental competence of porcine oocytes matured and fertilized in vitro.
Methods: Porcine zygotes were co-cultured with granulosa cells (GCs) (Group 1) or porcine oviductal epithelial cells (pOECs) at follicular stage (Group 2), ovulation stage (Group 3) or corpus luteum (CL) stage (Group 4) or cultured in a medium without co-culture cells (control group).
Results: The proportion of oocytes developed to 2-cell stage embryos in Group 2 was similar to that in control group, but significantly (p < 0.05) lower than that in Groups 1, 3 and 4. The proportions of oocytes developed to ≥4-cell stage embryos in Groups 3 and 4 were significantly (p < 0.05) higher than that in Groups 1 and 2. At 144 h after insemination, 12.0, 14.8 and 20.0% of oocytes developed to blastocysts in Groups 1, 3 and 4, respectively. However, no embryos in control group developed beyond 4-cell stage and no embryos in Group 2 developed to blastocyst stage.
Conclusion: As compared with GCs and pOECs at follicular stage, the pOECs at ovulation and CL stages had a better competence to support porcine embryo development under in vitro conditions.
Keywords: Co-culture, granulosa cells (GCs), in-vitro development, porcine embryos, porcine oviductal epithelial cells (pOECs)
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References
- 1.Vanroose G, Van Soom A, de Kruif A. From co-culture to defined medium: State of the art and practical considerations. Reprod Domest Anim. 2001;36(1):25–28. doi: 10.1046/j.1439-0531.2001.00264.x. [DOI] [PubMed] [Google Scholar]
- 2.Thompson JG. In vitro and embryo metabolism of cattle and sheep embryos—a decade of achievement. Anim Reprod Sci. 2000;60–61:263–275. doi: 10.1016/s0378-4320(00)00096-8. [DOI] [PubMed] [Google Scholar]
- 3.Kikuchi K, Naito K, Daen FP, Izaike Y, Toyoda Y. Histone H1 kinase activity during in vitro fertilization of pig follicular oocytes matured in vitro. Theriogenology. 1995;43:523–532. doi: 10.1016/0093-691x(94)00044-u. [DOI] [PubMed] [Google Scholar]
- 4.Rath D, Freitag K, Niemann H, Sieg B. Continuous control of porcine embryo development in vitro. Theriogenology. 1998;49:215. [Google Scholar]
- 5.Rath D. Experiments to improve in vitro fertilization techniques for in vivo matured porcine oocytes. Theriogenology. 1992;37:885–896. doi: 10.1016/0093-691x(92)90050-2. [DOI] [PubMed] [Google Scholar]
- 6.Koo DB, Kim NH, Lee HT, Chung KS. Effects of fetal calf serum, amino acids, vitamins and insulin on blastocel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro. Theriogenology. 1997;48:791–802. doi: 10.1016/s0093-691x(97)00302-6. [DOI] [PubMed] [Google Scholar]
- 7.Rath D, Niemann H, Torres CRL. In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro. Theriogenology. 1995;43:913–926. doi: 10.1016/0093-691x(95)00042-7. [DOI] [PubMed] [Google Scholar]
- 8.Dobrinsky, Johnson LA, Rath D. Development of a culture medium (BECM-3) for porcine embryos: Effects of bovine serum albumin and fetal bovine serum on embryo development. Biol Reprod. 1996;55:1069–1074. doi: 10.1095/biolreprod55.5.1069. [DOI] [PubMed] [Google Scholar]
- 9.Pushpakumara PG, Robinson RS, Demmers KJ, Mann GE, Sinclair KD, Webb R, Wathes DC. Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy. Reproduction. 2002;123(6):859–868. [PubMed] [Google Scholar]
- 10.Smith S, Schmidt M, Purwantara B, Greve T. Oviduct epithelial-cell co-culture of early porcine embryos. Acta Vet Scan. 1992;33:349–355. doi: 10.1186/BF03547301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Torres CRL, Rath D. In vitro culture of porcine embryos to the blastocyst stage after in vivo and in vitro fertilization. Theriogenology. 1992;37:283. [Google Scholar]
- 12.Kidson A, Schoevers E, Langendijk P, Verheijden J, Colenbrander B, Bevers M. The effect of oviductal epithelial cell co-culture during in vitro maturation on sow oocyte morphology, fertilization and embryo development. Theriogenology. 2003;59(9):1889–1903. doi: 10.1016/s0093-691x(02)01291-8. [DOI] [PubMed] [Google Scholar]
- 13.Gilbert CL, Cripps PJ, Wathes DC. The effect of oxytocin on the pattern of electromyographic activity in the oviduct and uterus of the ewe around oestrus. Reprod Fertil Dev. 1992;4:193–203. doi: 10.1071/rd9920193. [DOI] [PubMed] [Google Scholar]
- 14.Qian Y, Shi WQ, Ding JT, Sha JH, Fan BQ. Predictive value of the area of expanded cumulus mass on development of porcine oocytes matured and fertilized in vitro. J Reprod Dev. 2003;49(2):167–174. doi: 10.1262/jrd.49.167. [DOI] [PubMed] [Google Scholar]
- 15.Gardner DK, Schoolcraft WB. Human embryo viability: What determines developmental potential, and can it be assessed? J Assist Reprod Genet. 1998;15:455–458. doi: 10.1023/A:1022543901455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Pope WF, Xia S, Broermann DM, Nephew KP. Causes and consequences of early embryonic diversity in pigs. J Reprod Fertil. 1990;40(suppl):251–260. [PubMed] [Google Scholar]
- 17.Telford NA, Waeson AJ, Schultz GA. Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol Reprod Dev. 1990;26:90–100. doi: 10.1002/mrd.1080260113. [DOI] [PubMed] [Google Scholar]
- 18.Kane MT, Carney EW, Ellington JE. The role of nutrients, peptide growth factors and co-culture cells in development of preimplantation embryos in vitro. Theriogenology. 1992;38:297–313. doi: 10.1016/0093-691x(92)90237-l. [DOI] [PubMed] [Google Scholar]
- 19.White KL, Hehnke K, Rickords LF, Southern LL, Thompson DL, Wood TC. Early embryonic development in vitro by co-culture with oviductal epithelial cells in pigs. Biol Reprod. 1989;41:425–430. doi: 10.1095/biolreprod41.3.425. [DOI] [PubMed] [Google Scholar]
- 20.Bureau M, Bailey JL, Sirard MA. Influence of oviductal cells and conditioned medium on porcine gametes. Zygote. 2000;8:139–144. doi: 10.1017/s0967199400000915. [DOI] [PubMed] [Google Scholar]
- 21.Watson AJ, Watson PH, Warnes D, Walker SK, Armstrong DT, Seamark RF. Preimplantaion development of in-vitro matured and in-vitro fertilized ovine zygotes: Comparison oviduct epithelial cell monolayers and culture under low oxygen atmosphere. Biol Reprod. 1994;50:715–724. doi: 10.1095/biolreprod50.4.715. [DOI] [PubMed] [Google Scholar]