Abstract
Purpose:Our purpose was to evaluate whether pronuclei of unequal size, observed in 13.7% of zygotes evaluated after in vitro fertilization (IVF), are predictive of chromosome anomalies in the developing embryo.
Methods:The ploidy of 38 embryos grown from zygotes with unequal-sized pronuclei was assessed by fluorescent in situ hybridization (FISH). Twenty-six embryos developed after intracytoplasmic injection of sperm (ICSI) and 12 embryos were derived from conventional IVF.
Results:Chromosome anomalies were documented in the ICSI and IVF groups in 88.5 and 50% of cases, respectively.
Conclusions:We suggest that FISH should be employed to examine the ploidy of zygotes with unequal pronuclei, prior to embryo transfer.
Keywords: fluorescent in situ hybridization, in vitro fertilization, intracytoplasmic sperm injection, preimplantation diagnosis, pronuclear size
Full Text
The Full Text of this article is available as a PDF (833.1 KB).
REFERENCES
- 1.Harper JC, Coonen E, Handyside AH, De Paepe A, Dhont H. Mosaicism of autosomes and sex chromosomes in morphologically normal, monospermic preimplantation human embryos. Prenat Diagn. 1995;15:41–49. doi: 10.1002/pd.1970150109. [DOI] [PubMed] [Google Scholar]
- 2.Laverge H, De Sutter P, Verschraegen-Spae MR, et al. Triple color fluorescent in situ hybridization for chromosomes X, Y and 1 on spare human embryos. Hum Reprod. 1997;12:809–814. doi: 10.1093/humrep/12.4.809. [DOI] [PubMed] [Google Scholar]
- 3.Munné S, Sultan KM, Weier HU, Grifo JA, Cohen J, Rosenwaks Z. Assessment of numerical abnormalities of X, Y, 18 and 16 chromosomes in perimplantation human embryos prior to transfer. Am J Obstet Gynecol. 1995;172:1191–1120. doi: 10.1016/0002-9378(95)91479-x. [DOI] [PubMed] [Google Scholar]
- 4.Manor D, Kol S, Lewit N, et al. Undocumented embryos: Do not trash them, FISH them. Hum Reprod. 1996;11:2501–2506. doi: 10.1093/oxfordjournals.humrep.a019148. [DOI] [PubMed] [Google Scholar]
- 5.Sadowy S, Tomkin G, Munné S, Ferrara-Congedo T, Cohen J. Impaired development of zygotes with uneven pronuclear size. Zygote. 1998;6:137–141. doi: 10.1017/s0967199498000057. [DOI] [PubMed] [Google Scholar]
- 6.Van Belrkom J, Davis P, Merriam J, Sinclair J. Nuclear and cytoplasmic dynamics of sperm penetration, pronuclear formation and microtubule organization during fertilization and early preimplantation development in the human. Hum Reprod Update. 1995;5:429–461. doi: 10.1093/humupd/1.5.429. [DOI] [PubMed] [Google Scholar]
- 7.Sultan KM, Munné S, Palermo GD, Alikani M, Cohen J. Chromosomal status of unipronuclear human zygotes following invitro fertilization and intracytoplasmic sperm injection. Hum Reprod. 1995;10:132–139. doi: 10.1093/humrep/10.1.132. [DOI] [PubMed] [Google Scholar]
- 8.Munné S, Alikani M, Tomkin G, Grifo J, Cohen J. Embryo morphology, development rates, and maternal age are correlated with chromosome abnormalities. Fertil Steril. 1995;64:382–391. [PubMed] [Google Scholar]
- 9.Kligman I, Benadiva C, Alikani M, Munné S. The presence of multinucleated blastomeres in human embryos is correlated with chromosome abnormalities. Hum Reprod. 1996;11:1492–1498. doi: 10.1093/oxfordjournals.humrep.a019424. [DOI] [PubMed] [Google Scholar]
- 10.Delhanty JD, Harper JC, Ao A, Handyside AH, Winston RM. Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients. Hum Genet. 1997;99:755–760. doi: 10.1007/s004390050443. [DOI] [PubMed] [Google Scholar]