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. 1984 Jul;81(14):4311–4315. doi: 10.1073/pnas.81.14.4311

Ca2+/calmodulin-dependent protein kinases from the neuronal nuclear matrix and post-synaptic density are structurally related.

N Sahyoun, H LeVine 3rd, P Cuatrecasas
PMCID: PMC345578  PMID: 6589595

Abstract

A major Ca2+/calmodulin-dependent protein kinase has been isolated in association with the neuronal nuclear matrix. Nuclear matrix preparations contain highly phosphorylated polypeptides with Mr values of 50,000 and 60,000. These polypeptides were further characterized by peptide and phospho peptide mapping, two-dimensional isoelectrofocusing/NaDodSO4/PAGE, and 125I-labeled calmodulin binding. The results indicate that the Mr 50,000 and 60,000 polypeptides of the nuclear matrix closely resemble the alpha and beta subunits, respectively, of the Ca2+/calmodulin-dependent protein kinase of the post-synaptic density. These findings indicate that similar protein kinases mediate the neuronal effects of Ca2+ at the cytosolic, synaptosomal, and nuclear levels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agutter P. S., Richardson J. C. Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus. J Cell Sci. 1980 Aug;44:395–435. doi: 10.1242/jcs.44.1.395. [DOI] [PubMed] [Google Scholar]
  2. Baglia F. A., Maul G. G. Nuclear ribonucleoprotein release and nucleoside triphosphatase activity are inhibited by antibodies directed against one nuclear matrix glycoprotein. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2285–2289. doi: 10.1073/pnas.80.8.2285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett M. K., Erondu N. E., Kennedy M. B. Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain. J Biol Chem. 1983 Oct 25;258(20):12735–12744. [PubMed] [Google Scholar]
  4. Berezney R., Coffey D. S. Nuclear matrix. Isolation and characterization of a framework structure from rat liver nuclei. J Cell Biol. 1977 Jun;73(3):616–637. doi: 10.1083/jcb.73.3.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. Buttyan R., Olsson C. A., Sheard B., Kallos J. Steroid receptor-nuclear matrix interactions. The role of DNA. J Biol Chem. 1983 Dec 10;258(23):14366–14370. [PubMed] [Google Scholar]
  7. Caceres A., Bender P., Snavely L., Rebhun L. I., Steward O. Distribution and subcellular localization of calmodulin in adult and developing brain tissue. Neuroscience. 1983 Oct;10(2):449–461. doi: 10.1016/0306-4522(83)90145-8. [DOI] [PubMed] [Google Scholar]
  8. Carlin R. K., Bartelt D. C., Siekevitz P. Identification of fodrin as a major calmodulin-binding protein in postsynaptic density preparations. J Cell Biol. 1983 Feb;96(2):443–448. doi: 10.1083/jcb.96.2.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Carlin R. K., Grab D. J., Cohen R. S., Siekevitz P. Isolation and characterization of postsynaptic densities from various brain regions: enrichment of different types of postsynaptic densities. J Cell Biol. 1980 Sep;86(3):831–845. doi: 10.1083/jcb.86.3.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Carlin R. K., Grab D. J., Siekevitz P. Function of a calmodulin in postsynaptic densities. III. Calmodulin-binding proteins of the postsynaptic density. J Cell Biol. 1981 Jun;89(3):449–455. doi: 10.1083/jcb.89.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chafouleas J. G., Dedman J. R., Munjaal R. P., Means A. R. Calmodulin. Development and application of a sensitive radioimmunoassay. J Biol Chem. 1979 Oct 25;254(20):10262–10267. [PubMed] [Google Scholar]
  12. Ciejek E. M., Nordstrom J. L., Tsai M. J., O'Malley B. W. Ribonucleic acid precursors are associated with the chick oviduct nuclear matrix. Biochemistry. 1982 Sep 28;21(20):4945–4953. doi: 10.1021/bi00263a018. [DOI] [PubMed] [Google Scholar]
  13. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  14. Einck L., Bustin M. Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6735–6739. doi: 10.1073/pnas.80.22.6735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
  16. Goldenring J. R., Gonzalez B., McGuire J. S., Jr, DeLorenzo R. J. Purification and characterization of a calmodulin-dependent kinase from rat brain cytosol able to phosphorylate tubulin and microtubule-associated proteins. J Biol Chem. 1983 Oct 25;258(20):12632–12640. [PubMed] [Google Scholar]
  17. Igo-Kemenes T., Hörz W., Zachau H. G. Chromatin. Annu Rev Biochem. 1982;51:89–121. doi: 10.1146/annurev.bi.51.070182.000513. [DOI] [PubMed] [Google Scholar]
  18. Kelly P. T., McGuinness T. L., Greengard P. Evidence that the major postsynaptic density protein is a component of a Ca2+/calmodulin-dependent protein kinase. Proc Natl Acad Sci U S A. 1984 Feb;81(3):945–949. doi: 10.1073/pnas.81.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kennedy M. B., Bennett M. K., Erondu N. E. Biochemical and immunochemical evidence that the "major postsynaptic density protein" is a subunit of a calmodulin-dependent protein kinase. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7357–7361. doi: 10.1073/pnas.80.23.7357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kennedy M. B., McGuinness T., Greengard P. A calcium/calmodulin-dependent protein kinase from mammalian brain that phosphorylates Synapsin I: partial purification and characterization. J Neurosci. 1983 Apr;3(4):818–831. doi: 10.1523/JNEUROSCI.03-04-00818.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  23. Robinson S. I., Nelkin B. D., Vogelstein B. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Cell. 1982 Jan;28(1):99–106. doi: 10.1016/0092-8674(82)90379-8. [DOI] [PubMed] [Google Scholar]
  24. Rose K. M., Ruch P. A., Morris H. P., Jacob S. T. RNA polymerases from a rat hepatoma. Partial purification and comparison of properties with corresponding liver enzymes. Biochim Biophys Acta. 1976 Apr 15;432(1):60–72. doi: 10.1016/0005-2787(76)90041-1. [DOI] [PubMed] [Google Scholar]
  25. Saffer J. D., Glazer R. I. The phosphorylation of high mobility group proteins 14 and 17 and their distribution in chromatin. J Biol Chem. 1982 Apr 25;257(8):4655–4660. [PubMed] [Google Scholar]
  26. Shaper J. H., Pardoll D. M., Kaufmann S. H., Barrack E. R., Vogelstein B., Coffey D. S. The relationship of the nuclear matrix to cellular structure and function. Adv Enzyme Regul. 1978;17:213–248. doi: 10.1016/0065-2571(79)90015-3. [DOI] [PubMed] [Google Scholar]
  27. Thompson R. J. Studies on RNA synthesis in two populations of nuclei from the mammalian cerebral cortex. J Neurochem. 1973 Jul;21(1):19–40. doi: 10.1111/j.1471-4159.1973.tb04222.x. [DOI] [PubMed] [Google Scholar]
  28. Walton G. M., Spiess J., Gill G. N. Phosphorylation of high mobility group 14 protein by cyclic nucleotide-dependent protein kinases. J Biol Chem. 1982 Apr 25;257(8):4661–4668. [PubMed] [Google Scholar]
  29. Weisbrod S. Active chromatin. Nature. 1982 May 27;297(5864):289–295. doi: 10.1038/297289a0. [DOI] [PubMed] [Google Scholar]
  30. Yamauchi T., Fujisawa H. Purification and characterization of the brain calmodulin-dependent protein kinase (kinase II), which is involved in the activation of tryptophan 5-monooxygenase. Eur J Biochem. 1983 Apr 15;132(1):15–21. doi: 10.1111/j.1432-1033.1983.tb07319.x. [DOI] [PubMed] [Google Scholar]

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